Isolation and Characterization of Lipid-Degrading Bacteria in Wastewater of Food Processing Plants and Restaurants in Can Tho City, Vietnam

Abstract

High lipid (fats and oils) concentration contained in wastewater inhibits the activity of microbes in biological wastewater treatment systems. The lipids degradation capability of lipid-degrading bacteria was investigated for possible application in treatment of lipids-contaminated wastewater. One hundred and two bacterial isolates were isolated from 43 vegetable oil-contaminated wastewater samples of many food processing plants and restaurants in 5 districts of Can Tho city, Vietnam on LB medium. There were sixty-one isolates produced clear zones on Tw20 medium, only eleven of which were found to have the high ability to degrade vegetable oil in the contaminated wastewater. These eleven isolates were identified by PCR technique and DNA sequencing. The results of DNA sequencing were compared with GenBank database of NCBI by BLAT N software. The sequences from selected isolates showed high degrees of similarity to those of the GenBank references (between 97% and 99%). Two isolates belonged to Bacilli (18.18%) and nine isolates belonged to Gammaproteobacteria (81.82%). Based on Pi value (nucleotide diversity), Gammaproteobacteria group had the highest Theta values. Theta value (per sequence) from S of SNP for DNA polymorphism were calculated for each group and 11 strains of lipid-degrading bacteria had high genetic diversity. The results propose Acinetobacteria soli strain AL3 a potential bioproduct for wastewater treatment because of its high ability of lipid degradation and biosafety.

The Impact of a Fish Cannery Wastewater Discharge on the Bacterial Community Structure and Sanitary Conditions of Marine Coastal Sediments

“…The phyla Firmicutes and Gammaproteobacteria dominated the bacterial community of the FCD effluent. Acinetobacter, the most abundant gammaproteobacterial genus in the FCD, has been shown to produce extracellular lipase enzymes that hydrolyze triglycerides (the main component of oils and fats) to fatty acids and glycerol [3,5]. Also, the genera Pseudomonas and Bacillus too abundant in the FCD have been found to have a potential role in the process of partial or complete cholesterol degradation [4].…”

“…The bacterial community of such effluents can be enriched with phyla such as Bacteroidetes, Chloroflexi, Thermotogae, Synergystetes, and Firmicutes (in a case study of a tuna cannery) which are often characteristic of anaerobic environments [2]. Fish cannery discharges can also contain specific bacterial groups able to produce extracellular lipase enzymes that hydrolyze triglycerides and cholesterol [3][4][5][6][7][8]. Moreover, faecal indicator bacteria and various potentially pathogenic genera [9] are commonly very abundant in such effluents, indicating their negative impact on the sanitary quality of the receiving environment.…”

Formulation and Application of Molasses-Based Bacterial Consortium to Degrade Waste Oil and Fat in the Environment

“…Limbah lemak dapat berasal dari lemak hewan, gabungan dari gliserol dan asam lemak bebas serta minyak sayur. Phong et al, 2014) Bali sebagai kota pariwisata terbesar di Indonesia dengan banyak hotel dan villa tak luput dari permasalahan tersebut mengingat sampai saat ini pembuangan limbah lemak masih belum terkontrol dengan baik. Formulasi media sangatlah penting sebagai nutrisi bagi bakteri yang bertindak sebagai bioremediasi limbah lemak dan minyak pada lingkungan.…”

Journal of Water and Land Development

“…To this end, 10 cm 3 bacterial isolates were inoculated into 200 cm 3 wastewater in 500-cm 3 plastic bottles, and then the treatment was incubated at 30°C for a week. The experiment was done according to PHONG et al [2014].…”