Isolation and Characterization of Innate Lymphoid Cells within the Murine Tumor Microenvironment

Mallett, Grace; Patterson, Warren; Payne, Megan; Amarnath, Shoba

doi:10.1007/978-1-0716-0338-3_14

Cited by 2 publications

(1 citation statement)

References 23 publications

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“…ILCs from the tumor tissue were isolated as previously described 58 . Briefly, tumor tissue was incubated at 37 o C for 30minutes in FBS free DMEM media containing liberase TM TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml; Roche).…”

Section: Til Isolationmentioning

confidence: 99%

Programmed cell death-1 receptor mediated regulation of Tbet⁺ NK1.1⁻ Innate Lymphoid Cells within the Tumor Microenvironment

Lim

Lai

Mallett

et al. 2022

Preprint

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Innate Lymphoid Cells (ILCs) play a key role in tissue mediated immunity and can be controlled by co-receptor signaling. Here we define a subset of ILCs that are Tbet+NK1.1- and are present within the tumor microenvironment (TME). We show programmed death-1 receptor (PD-1) expression on ILCs within TME is found in Tbet+NK1.1-ILCs. PD-1 significantly controlled the proliferation and function of Tbet+NK1.1-ILCs in multiple murine and human tumors. We found tumor derived lactate enhanced PD-1 expression on Tbet+NK1.1-ILCs within the TME, which resulted in dampened mTOR signaling along with increased fatty acid uptake. In line with these metabolic changes, PD-1 deficient Tbet+NK1.1-ILCs expressed significantly increased IFNg, granzyme B and K. Furthermore, PD1 deficient Tbet+NK1.1- ILCs contributed towards diminished tumor growth in an experimental murine model of melanoma. These data demonstrate that PD-1 can regulate anti-tumor responses of Tbet+NK1.1-ILCs within the tumor microenvironment.

show abstract

Section: Til Isolationmentioning

confidence: 99%

Programmed cell death-1 receptor mediated regulation of Tbet⁺ NK1.1⁻ Innate Lymphoid Cells within the Tumor Microenvironment

Lim

Lai

Mallett

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Programmed cell death-1 receptor-mediated regulation of Tbet ⁺ NK1.1 ⁻ innate lymphoid cells within the tumor microenvironment

Lim

Lai

Mallett

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

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Innate lymphoid cells (ILCs) play a key role in tissue-mediated immunity and can be controlled by coreceptor signaling. Here, we define a subset of ILCs that are Tbet + NK1.1 − and are present within the tumor microenvironment (TME). We show programmed death-1 receptor (PD-1) expression on ILCs within TME is found in Tbet + NK1.1 − ILCs. PD-1 significantly controlled the proliferation and function of Tbet + NK1.1 − ILCs in multiple murine and human tumors. We found tumor-derived lactate enhanced PD-1 expression on Tbet + NK1.1 − ILCs within the TME, which resulted in dampened the mammalian target of rapamycin (mTOR) signaling along with increased fatty acid uptake. In line with these metabolic changes, PD-1-deficient Tbet + NK1.1 − ILCs expressed significantly increased IFNγ and granzyme B and K. Furthermore, PD-1-deficient Tbet + NK1.1 − ILCs contributed toward diminished tumor growth in an experimental murine model of melanoma. These data demonstrate that PD-1 can regulate antitumor responses of Tbet + NK1.1 − ILCs within the TME.

show abstract

Isolation and Characterization of Innate Lymphoid Cells within the Murine Tumor Microenvironment

Cited by 2 publications

References 23 publications

Programmed cell death-1 receptor mediated regulation of Tbet⁺ NK1.1⁻ Innate Lymphoid Cells within the Tumor Microenvironment

Programmed cell death-1 receptor mediated regulation of Tbet⁺ NK1.1⁻ Innate Lymphoid Cells within the Tumor Microenvironment

Programmed cell death-1 receptor-mediated regulation of Tbet ⁺ NK1.1 ⁻ innate lymphoid cells within the tumor microenvironment

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Isolation and Characterization of Innate Lymphoid Cells within the Murine Tumor Microenvironment

Cited by 2 publications

References 23 publications

Programmed cell death-1 receptor mediated regulation of Tbet+ NK1.1− Innate Lymphoid Cells within the Tumor Microenvironment

Programmed cell death-1 receptor mediated regulation of Tbet+ NK1.1− Innate Lymphoid Cells within the Tumor Microenvironment

Programmed cell death-1 receptor-mediated regulation of Tbet + NK1.1 − innate lymphoid cells within the tumor microenvironment

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Programmed cell death-1 receptor mediated regulation of Tbet⁺ NK1.1⁻ Innate Lymphoid Cells within the Tumor Microenvironment

Programmed cell death-1 receptor mediated regulation of Tbet⁺ NK1.1⁻ Innate Lymphoid Cells within the Tumor Microenvironment

Programmed cell death-1 receptor-mediated regulation of Tbet ⁺ NK1.1 ⁻ innate lymphoid cells within the tumor microenvironment