Isolation and characterization of
            <i>Salmonella</i>
            in pork samples collected from retail and wholesale markets in each season from 2016 to 2018 in Wuhan, China

Zhu, Zhihao; Huang, Qi; Hong, Xuan; Chen, Xingyu; Lu, Yangchao; Zhou, C.; Wang, Chunfang; Meng, Xianrong; Xu, Qingrong; Li, Shaowen

doi:10.1111/jam.14515

Cited by 13 publications

(5 citation statements)

References 31 publications

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“…Similarly, all Salmonella isolates were resistant to the 11 antimicrobial agents in this study. For each antimicrobial agent, the antimicrobial with the highest resistance rate in this study was ampicillin, which was consistent with the results of reports from Wuhan (Zhu et al., 2020 ) and Yangzhou (Li, Yin, et al., 2019 ). The resistance of Salmonella to ampicillin has become commonplace in Africa and the USA owing to its extensive clinical use (Ke et al., 2014 ).…”

Section: Discussionsupporting

confidence: 92%

Occurrence and antimicrobial resistance of Salmonella isolated from retail meats in Anhui, China

Chen

Shao

et al. 2021

Food Science & Nutrition

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Section: Discussionsupporting

confidence: 92%

Occurrence and antimicrobial resistance of Salmonella isolated from retail meats in Anhui, China

Chen

Shao

et al. 2021

Food Science & Nutrition

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“…Salmonella Rissen ST469 was isolated from ready-to-eat mussels in Spain and pork products in Portugal [ 35 , 36 ]. Moreover, ST469 was the third most predominant ST isolated from pork samples in China and nearly one-third of the ST469 isolates were resistant to ciprofloxacin [ 37 ]. However, the sequence type of one Salmonella Derby isolate could not be determined by MLST, suggesting the possibility that a potential novel ST of Salmonella Derby has emerged in South Korea.…”

Section: Resultsmentioning

confidence: 99%

Presence of plasmid-mediated quinolone resistance (PMQR) genes in non-typhoidal Salmonella strains with reduced susceptibility to fluoroquinolones isolated from human salmonellosis in Gyeonggi-do, South Korea from 2016 to 2019

et al. 2021

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Non-typhoidal salmonellosis remains a pressing public health problem worldwide. Quinolones, particularly fluoroquinolones, are widely used to treat various infections, including non-typhoidal salmonellosis, which can be a serious illness. The emergence of fluoroquinolone-resistant Salmonella has resulted in treatment failure and high mortality rates. In this study, we estimated the presence of plasmid-mediated quinolone resistance (PMQR) genes in Salmonella enterica isolated from human salmonellosis patients in South Korea from 2016 to 2019. We evaluated the association of these genes with fluoroquinolone susceptibility. Antimicrobial susceptibility tests for Salmonella isolates were performed using the Vitek II system, and the minimum inhibitory concentrations (MIC) of ciprofloxacin and levofloxacin were determined using the E-test method. Plasmid-mediated quinolone resistance (PMQR) genes were detected by PCR amplification and quinolone resistance-determining regions (QRDRs) of the gyrA and parC genes were analyzed following Sanger sequencing of the PCR products. Thirty-four Salmonella strains with reduced susceptibility to fluoroquinolones (ciprofloxacin MIC ≥ 0.125 µg/mL and levofloxacin MIC ≥ 0.25 µg/mL) were selected from 208 human clinical Salmonella isolates. Among them, 22 Salmonella strains harbored one PMQR gene (qnrA, qnrB, or qnrS), and three Salmonella strains carried two PMQR genes (qnrS and aac(6′)-Ib-cr or qnrA and qnrB). qnrS was the most common PMQR gene. Serotyping revealed that Salmonella 4,[5]12:i:- (32.4%, 11/34) and Salmonella Typhimurium (29.4%, 10/34) were the two most predominant serovars, and Multi-locus sequence typing (MLST) showed that ST19 and ST34 were the most frequent sequence types. In conclusion, qnr gene-positive Salmonella 4,[5],12:i:- and Salmonella Typhimurium were the main serovars responsible for reduced susceptibility to fluoroquinolones. Therefore, our findings suggest that PMQR-positive Salmonella strains, which can be isolated from various samples including human, food, and the environment, should be carefully monitored.

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“…In recent years, a large number of epidemiological surveys on Salmonella have emerged in China. However, researchers were more inclined to conduct their epidemiological Salmonella surveys on commercial broiler sales chains where sick birds appeared than on healthier chains (15)(16)(17). Therefore, data from epidemiological surveys on Salmonella in domestic poultry farms, especially chicken breeder flocks, are incomplete.…”

Section: Introductionmentioning

confidence: 99%

Occurrence and Characterization of Salmonella Isolated From Chicken Breeder Flocks in Nine Chinese Provinces

et al. 2020

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We investigated the prevalence of salmonellosis on 17 poultry breeding farms in nine Chinese provinces (Shandong, Jiangsu, Anhui, Zhejiang, Fujian, Guangdong, Yunnan, Sichuan, and Chongqing). Altogether, 3,508 samples from poultry breeding farms were collected in 2019, including 1,400 from cloaca swabs, 210 from feed, 1,688 from chicken embryos, and 210 from water. All the samples were subjected to bacterial isolation and culture, and bacterial species were identified by polymerase chain reaction. Serotyping, multilocus sequence typing (MLST), and drug-resistance phenotyping were performed on the isolates identified as Salmonella . Altogether, 126 Salmonella strains were detected in the 3,508 samples and the positivity rate for the samples was 3.59%. Among all the strains, 95 Salmonella isolates were selected for antimicrobial susceptibility test, resistance gene detection, serotyping, and genotyping. S. gallinarum-pullorum (57/95, 60.00%), S. enteritidis (22/95, 23.16%), and S. agona (16/95, 16.84%) serotypes were identified. The MLST classification showed that the 95 Salmonella strains fell into the following five sequence types (STs): ST92 (37/95, 38.95%), ST11 (22/95, 23.16%), ST2151 (19/95, 20.00%), ST13 (16/95, 16.84%), and ST470 (1/95, 1.05%). Apart from ST13, the other four STs shared close genetic relationships, and the genetic direction was ST11-ST470-ST92-ST2151. The resistance rates in the 95 isolates were 100% (95/95) for erythromycin, 68.42% (65/95) for tetracycline, and 53.68% (51/95) for streptomycin and ampicillin, respectively. The isolates were sensitive to polymyxin and sulfamethoxazole. Multi-drug resistance was seen in 70.53% (67/95) of the isolates. β-lactam-, aminoglycoside- and sulfonamide-encoding resistance genes were detected by PCR. The detection rate for bla TEM and sul3 was 100% (95/95), whereas sul2 and aaC4 had rates of 52.63 and 23.16%, respectively. These results indicate that some of the salmonellosis seen in Chinese breeding chicken farms may be caused by infection with S. gallinarum-pullorum, S. enteritidis , and S. agona . They also show that some Salmonella isolates have multi-drug resistance phenotypes and carry multi-drug resistance genes.

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Isolation and characterization of Salmonella in pork samples collected from retail and wholesale markets in each season from 2016 to 2018 in Wuhan, China

Cited by 13 publications

References 31 publications

Occurrence and antimicrobial resistance of Salmonella isolated from retail meats in Anhui, China

Occurrence and antimicrobial resistance of Salmonella isolated from retail meats in Anhui, China

Presence of plasmid-mediated quinolone resistance (PMQR) genes in non-typhoidal Salmonella strains with reduced susceptibility to fluoroquinolones isolated from human salmonellosis in Gyeonggi-do, South Korea from 2016 to 2019

Occurrence and Characterization of Salmonella Isolated From Chicken Breeder Flocks in Nine Chinese Provinces

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