Isolation and characterization of Klebsiella oxytoca strain degrading crude oil from a Tunisian off‐shore oil field

Abstract

A facultatively anaerobic, Gram-negative, mesophilic, moderately halotolerant, non-motile, and non-sporulated bacterium, designated strain BSC5 was isolated from an off-shore "Sercina" oil field, located near the Kerkennah island, Tunisia. Yeast extract was not required for growth. Phenotypic characteristics and phylogenetic analysis of the 16S rRNA gene sequence of strain BSC5 revealed that it was related to members of the genus Klebsiella, being most closely related to the type strain of K. oxytoca (99% sequence similarity). Strain BSC5 was capable of using aerobically the crude oil as substrate growth. The growth of strain BSC5 on crude oil was followed by measuring the OD(600 nm) and by enumeration of viable cells at different culture's time. GC-MS analysis showed that strain BSC5 was capable of degrading a wide range of aliphatic hydrocarbons from C(13) to C(30) . The biodegradation rate for n -alkanes reached 44% and 75%, after 20 and 45 days of incubation, respectively. Addition of the synthetic surfactant, Tween 80, accelerated the crude oil degradation. The biodegradation rate for n -alkanes reached 61% and 98%, after 20 and 45 days of incubation, respectively. Moreover, three aromatic compounds, p -hydroxybenzoate, protocatechuate and gentisate, were metabolized completely by strain BSC5 after 24 h, under aerobic conditions.

Isolation and Characterization of Hydrocarbon-utilizing Bacteria from Petroleum Sludge Samples obtained from Crude Oil Processing Facility in Nigeria

“…The results of isolation and identification of Klebsiella Pneumonia and K. oxytoca to the strain levels in this study, agree with previous studies that implicated the same organisms in petroleum hydrocarbon degradation. Chamkha et al, (2011) reported the isolation and characterization of Klebsiella oxytoca strain degrading crude oil from Tunisian off-shore oil fields, similarly, Rodrigues et al, (2009) had earlier reported diversity of hydrocarbon-degrading Klebsiella strains isolated from hydrocarbon-contaminated estuaries. The oildegrading Klebsiella isolates obtained from the estuary were closely related to Klebsiella pneumonia and Klebsiella ornithinnolytica.…”

“…Fortunately, microorganisms are highly efficient and versatile in their ability to degrade hydrocarbons; (Odokuma and Dickson 2003;Head et al, 2006;Odokuma and Smith 2007;Adebusoye et al, 2007). Bacteria that biodegrade polynuclear aromatic hydrocarbons (PAHs), monoaromatic hydrocarbons and aliphatic hydrocarbons are readily isolated from the environment that have been contaminated with petroleum hydrocarbon (Chamkha et al, 2011;Manchola and Dussan, 2014). A reviewed list of bacterial genera has listed 79 genera that can utilize hydrocarbon as the sole source of carbon and energy (Head et al, 2006).…”

Isolation and molecular identification of polyaromatic hydrocarbons- utilizing bacteria from crude petroleum oil samples

“…Moreover, in a previous study Acinetobacter sp which has been successfully grown on crude oil was molecularly identified using only the first 5´ end (approx 821 bp) of the 16S rDNA (Koren et al, 2003). Besides, Chamkha et al (2011) emphasized that this gene is a strong chronometer for identification of a K. oxytoca strain BSC5 which has been recently isolated from Sercina oil field, Tunisia. This strain was able to grow on crude oil without a need to a nitrogen source.…”

Petroleum Hydrocarbon Spills in the Environment and Abundance of Microbial Community Capable of Biosurfactant Production

“…Pseudomonadaceae (94%) was the most dominant family from the sample whereas Enterobacteriaceae (0.018%) and Xanthomonadaceae (0.0026%) were observed at 1% cut off level (Figure 1; Relative abandance). Proteobacteria represented 88.8% of the phyla in the ENGB whereas Firmicutes (2.22%), Actinobacteria (1.6%), (Figure 2a) representative genera in this family like Klebsiella and Serratia were reported as efficient biosurfactant producers and capable of hydrocarbon degradation [39,40]. Pseudomonas spp which are dominant in sample A (Figure 2b) are able to endure and metabolize contaminants that are considered very toxic to other bacteria.…”