Isolation and characterization of <i>Bordetella pseudohinzii</i> in mice in China

Ma, Lei; Huang, Shuwu; Luo, Yinzhu; Min, Fangui; He, Ling; Chen, Mei‐Ling; Pan, Jinchun; Wang, Jing

doi:10.1002/ame2.12075

Cited by 3 publications

(3 citation statements)

References 20 publications

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“…As a new member of the genus Bordetella, B. pseudohinzii was first identified and isolated from laboratory-raised mice and has now been detected in mouse facilities worldwide. 83 The infected mice presented elevated numbers of neutrophils in bronchoalveolar lavage fluid and inflammatory signs in histopathology, although no obvious clinical symptoms were shown. 84 These causative agents may induce more severe inflammation through cooperation with intestinal microbes.…”

Section: Discussionmentioning

confidence: 94%

Imbalance of the intestinal virome and altered viral-bacterial interactions caused by a conditional deletion of the vitamin D receptor

Zhang

Xia

et al. 2021

Gut Microbes

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Vitamin D receptor (VDR) deficiency is associated with cancer, infection, and chronic inflammation. Prior research has demonstrated VDR regulation of bacteria; however, little is known regarding VDR and viruses. We hypothesize that VDR deficiency impacts on the intestinal virome and viral-bacterial interactions. We specifically deleted VDR from intestinal epithelial cells (VDR ΔIEC ), Paneth cells (VDR ΔPC ), and myeloid cells (VDR ΔLyz ) in mice. Feces were collected for shotgun metagenomic sequencing and metabolite profiling. To test the functional changes, we evaluated pattern recognition receptors (PRRs) and analyzed microbial metabolites. Vibrio phages, Lactobacillus phages, and Escherichia coli typing phages were significantly enriched in all three conditional VDR-knockout mice. In the VDR ΔLyz mice, the levels of eight more virus species (2 enriched, 6 depleted) were significantly changed. Altered virus species were primarily observed in female VDR ΔLyz (2 enriched, 3 depleted) versus male VDR ΔLyz (1 enriched, 1 depleted). Altered alpha and beta diversity (family to species) were found in VDR ΔLyz . In VDR ΔIEC mice, bovine viral diarrhea virus 1 was significantly enriched. A significant correlation between viral and bacterial alterations was found in conditional VDR knockout mice. There was a positive correlation between Vibrio phage JSF5 and Cutibacterium acnes in VDR ΔPC and VDR ΔLyz mice. Also, there were more altered viral species in female conditional VDR knockout mice. Notably, there were significant changes in PRRs: upregulated TLR3, TLR7, and NOD2 in VDR ΔLyz mice and increased CLEC4L expression in VDR ΔIEC and VDR ΔPC mice. Furthermore, we identified metabolites related to virus infection: decreased glucose in VDR ΔIEC mice, increased ribulose/xylulose and xylose in VDR ΔLyz mice, and increased long-chain fatty acids in VDR ΔIEC and VDR ΔLyz female mice. Tissue-specific deletion of VDR changes the virome and functionally changes viral receptors, which leads to dysbiosis, metabolic dysfunction, and infection risk. This study helps to elucidate VDR regulating the virome in a tissue-specific and sex-specific manner.

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Section: Discussionmentioning

confidence: 94%

Imbalance of the intestinal virome and altered viral-bacterial interactions caused by a conditional deletion of the vitamin D receptor

Zhang

Xia

et al. 2021

Gut Microbes

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“…Meanwhile, Bordetella pseudohinzii showed a positive correlation with Vibrio phage JSF5. As a new member of the genus Bordetella, B. pseudohinzii was rst identi ed and isolated from laboratory-raised mice and has now been detected in mouse facilities worldwide [74]. The infected mice presented elevated numbers of neutrophils in bronchoalveolar lavage uid and in ammatory signs in histopathology, although no obvious clinical symptoms were shown [75].…”

Section: Discussionmentioning

confidence: 99%

Imbalance of the intestinal virome caused by a conditional deletion of the vitamin D receptor from epithelial cells, Paneth cells, or myeloid cells in murine models

Zhang

Xia

et al. 2021

Preprint

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Background Vitamin D receptor (VDR) is highly conserved in mammals, and its deficiency is associated with various diseases (e.g., cancer, infection, and chronic inflammation). Prior research has described the effect of VDR on bacteria; however, little is known regarding the effects of VDR on viruses. We hypothesize that VDR is a regulator of the virome and virus-bacterial interactions. We conditionally deleted VDR from intestinal epithelial cells (VDRΔIEC), Paneth cells (VDRΔPC), and myeloid cells (VDRΔLyz) in mice. Feces were collected for shotgun metagenomic sequencing and metabolite profiling. To test the functional changes, we evaluated pattern recognition receptors (PRRs) and analyzed microbial metabolites. Results Vibrio phages, Lactobacillus phages and Escherichia coli typing phages were significantly enriched in all three conditional VDR-knockout mice compared to VDRLoxP mice. In the VDRΔLyz mice, the levels of eight more virus species (2 enriched, 6 depleted) were significantly changed. These significantly altered virus species were primarily observed in female VDRΔLyz (2 enriched, 3 depleted) versus male VDRΔLyz (1 enriched, 1 depleted). Altered alpha and beta diversity were found in VDRΔLyz (family to species). In VDRΔIEC mice, bovine viral diarrhea virus 1 was significantly enriched. Overall, these significantly altered virus species were more often observed in female VDRΔIEC mice (2 enriched, 5 depleted) versus male mice (none). A significant correlation between viral and bacterial alterations was found in conditional VDR knockout mice. There was a positive correlation between Vibrio phage JSF5 and Cutibacterium acnes in VDRΔPC and VDRΔLyz mice. Also, there were more altered viral species in female conditional VDR knockout mice. Notably, there were significant changes in PRRs: upregulated TLR3, TLR7, and NOD2 in VDRΔLyz mice and increased CLEC4L expression in VDRΔIEC and VDRΔPC mice. Furthermore, we identified metabolites related to virus infection: decreased glucose in VDRΔIEC mice, increased ribulose/xylulose and xylose in VDRΔLyz mice, and increased long-chain fatty acids in VDRΔIEC and VDRΔLyz female mice. Conclusion Tissue-specific deletion of VDR changes the virome and functionally changes viral receptors, which leads to dysbiosis, metabolic dysfunction, and infection risk. This study helps to elucidate VDR regulation of the virome in a tissue-specific and sex-specific manner.

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“…The N terminus of its septation protein A, a transmembrane protein involved in cell division, starts with the MKKFRrelated sequence MKKFLFD (UniProt in silico analysis). This recently identified Bordetella species has not yet found entry into the Federation for Laboratory Animal Science Associations (FELASA) recommendation list for the health monitoring of mouse colonies in experimental units and occurs widely spread, often unrecognized, in animal facilities (Hayashimoto et al, 2012;Ma et al, 2019). We recently reported an outbreak of B. pseudohinzii with tracheitis and bronchointerstitial pneumonia in one of our animal houses (Perniss et al, 2018).…”

Section: Susceptibility To Tracheal Colonization By Pathogens Is Highmentioning

confidence: 99%

Chemosensory Cell-Derived Acetylcholine Drives Tracheal Mucociliary Clearance in Response to Virulence-Associated Formyl Peptides

et al. 2020

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Isolation and characterization of Bordetella pseudohinzii in mice in China

Cited by 3 publications

References 20 publications

Imbalance of the intestinal virome and altered viral-bacterial interactions caused by a conditional deletion of the vitamin D receptor

Imbalance of the intestinal virome and altered viral-bacterial interactions caused by a conditional deletion of the vitamin D receptor

Imbalance of the intestinal virome caused by a conditional deletion of the vitamin D receptor from epithelial cells, Paneth cells, or myeloid cells in murine models

Chemosensory Cell-Derived Acetylcholine Drives Tracheal Mucociliary Clearance in Response to Virulence-Associated Formyl Peptides

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