Isolation and Characterization ofAcidithiobacillus ferrooxidansStrain D3-2 Active in Copper Bioleaching from a Copper Mine in Chile

Sulfite Oxidation Catalyzed byaa3-Type CytochromecOxidase inAcidithiobacillus ferrooxidans

“…23) In contrast, A. ferrooxidans strain AP19-3 is the most sensitive to sulfite among the strains tested. 18,20,24,25,32,33,38) Sulfite oxidase activities were compared with cytochrome c oxidases purified from A. ferrooxidans strains D3-2, ATCC 23270, MON-1, and AP19-3 as enzyme sources.…”

“…The ironoxidizing bacteria used in this study were A. ferrooxidans D3-2, 23) A. ferrooxidans ATCC 23270, A. ferrooxidans MON-1, [30][31][32] and A. ferrooxidans AP19-3.…”

“…ferrooxidans strain D3-2, which was isolated from a low-grade sulfide ore dump at a copper mine in Chile, had approximately 3-fold higher Cu-bioleaching activity but 3-fold lower iron oxidase activity than that of A. ferrooxidans ATCC 23270 cells. 23) During study of this phenomenon, we found that A. ferrooxidans D3-2 had sulfite resistant-sulfite oxidase. 23) Sulfite is an intermediate produced during sulfur oxidation in y To whom correspondence should be addressed.…”

“…23) During study of this phenomenon, we found that A. ferrooxidans D3-2 had sulfite resistant-sulfite oxidase. 23) Sulfite is an intermediate produced during sulfur oxidation in y To whom correspondence should be addressed. Tel/Fax: +81-86-251-8306; E-mail: sugio@cc.okayama-u.ac.jp A. ferrooxidans and is harmful to iron oxidase and SFORase, 13,[15][16][17]21,[24][25][26][27][28] but there have been few reports on the enzyme involved in sulfite oxidation in A. ferrooxidans.…”

“…A marked difference was observed in the activity of the enzyme involved in the first step of sulfur oxidation in A. ferrooxidans, viz., SFORase. 22,23) The A. ferrooxidans strains, which have higher SFORase activity than other A. ferrooxidans strains, showed higher Cu-bioleaching activity. 22) In contrast, the A. ferrooxidans strains, which have lower SFORase activity, showed lower Cu-bioleaching activity.…”