Isolation and Characterization of Hydrocarbon-Degrading Bacteria from Wastewaters in Ouagadougou, Burkina Faso

Abstract

Lubricants are very often found in nature under waste-oil forms and represent for the environment a real danger of pollution due to the difficulty of their biodegradation. The situation is especially worrying in most developing countries in particular those of Sub-Saharan Africa due to the absence of regulation or control. The present work aims to isolate bacterial strains able to degrade hydrocarbons which can later be used in biotechnology for environments depollution. Oil-contaminated wastewater samples were collected in Ouagadougou city (Burkina Faso) and then used as source of bacterial isolation. Appropriate amounts of samples were inoculated to a mineral salt medium (MS) with Total Quartz 9000 oil as sole carbon and energy source and then incubated for enrichment, prior to microbe isolation. Two bacterial strains namely S2 and S 7 were isolated from the enrichment cultures. The strains were tested for their ability to degrade other hydrocarbons (i.e. gasoline, diesel oil, brake oil) and for temperature, pH and salt concentration ranges for growth before their biochemical characteristics were defined. Based on their morphological, physiological and biochemical traits, strains S 2 and S 7 belong to Acinetobacter and Pseudomonas genera, respectively.

Optimization of Hydrocarbons Biodegradation by Bacterial Strains Isolated from Wastewaters in Ouagadougou, Burkina Faso: Case Study of SAE 40/50 Used Oils and Diesel

“…This result could be explained by the high growth rates of strain S2 compared to those of strain S7 [19]. This relation between strain growth rate and hydrocarbon biodegradation was demonstrated by Mandri and Lin [25].…”

“…Based on their morphological, biochemical and physiological characteristics, the strains S7 and S2 appeared to be related to Pseudomonas and Acinetobacter genera, respectively [19].…”

“…The gravimetric estimation of residual oil left after biodegradation was made by weighing the quantity of oil in a tarred beaker. The percentage of biodegraded oil was then evaluated in comparison to the initial hydrocarbon amount in the control funnel according to Fusey and Oudot [24], as described by Sawadogo et al [19].…”

“…In our previous works, two bacterial strains able to degrade hydrocarbons were isolated [19]. The optimization of Total Quartz SEA 40 used oil and diesel oil biodegradation by these strains in presence of nutrient factors was also performed [20].…”

Trends in mean maximum temperature, mean minimum temperature and mean relative humidity for Lautoka, Fiji during 2003–2013

“…Trends in mean maximum temperature, mean minimum temperature and mean relative humidity for Lautoka, Fiji during 2003-2013 than the average seasonal maximum temperatures (Daly, Conklin & Unsworth, 2010;Tang & Arnone, 2013;Sawadogo et al 2014). These changes were related to numerous reasons like global warming, amplifi ed concentrations of anthropogenic greenhouse gases, aerosols (that may also wield cooling effects on the climate in various circumstances), enhanced cloud cover and development.…”