Isolation and characterization of hydrocarbon-degrading bacteria from gas station leaking-contaminated groundwater in the Southern Amazon, Brazil

Abstract

<jats:p>Abstract Twenty-three hydrocarbon-degrading bacteria strains were isolated from gas station leaking-contaminated groundwater located in the Southern Amazon, Brazil. Based on hydrocarbon (diesel, hexadecane, benzene, toluene and xylene) degradation ability, two strains were selected for further study. The amplification and sequencing of the 16S rRNA gene showed that these two strains belonged to the genus Bacillus (Bacillus sp. L26 and Bacillus sp. L30). GC-MS analysis showed that strain L30 was the most effective in degrading n-alkane (C10-C27) from diesel after 7 days of cultivation in mineral medium. Both strains produced biosurfactants and showed emulsification activity, specially the strain L30. Alkane hydroxylase gene (group III), which is important for alkane biodegradation, was present in strains. As a result, this study indicated that these bacteria could have promising applications in hydrocarbon bioremediation.</jats:p>

Comparison of petroleum biodegradation efficiencies of three different bacterial consortia determined in petroleum-contaminated waste mud pit

“…However, Bacillus sp. L30 strain was the most effective bacterium in degrading n-alkane (C 10 -C 27 ) from diesel after 7 days of incubation [43]. Parthipan et al [44] stated that more than 97% of the n-alkanes ranged from C 15 to C 19 were utilized by the B. subtilis A1.…”

Isolation and characterization of diesel-degrading bacteria from hydrocarbon-contaminated sites, flower farms and soda lakes

“…This approach mainly relies on two main techniques viz. bioaugmentation and biostimulation [24]. The bioaugmentation involves an introduction of selected hydrocarbondegrading microbial strains or consortia to the polluted environment to boost the already existing potential microbial communities for the biodegradation process [1,24,27,38,44].…”

“…Biostimulation, however, engages the amendment of macro-and micronutrients, sustains physical parameters (pH, temperature and aeration) and supplies surface-active substances (surfactants) in contaminated sites to optimize soil conditions and enhance biodegradation by increasing the growth rate of inhabitant hydrocarbon-degrading microorganisms [16,24,29,34,36,38,44]. This technique can be applied in situ and ex situ [27] to enhance biodegradation by increasing the bioavailability of the pollutants and the growth rate of inhabitant (indigenous, autochthonous) hydrocarbon-degrading microorganisms [1].…”

“…These include mechanical, chemical, and biological approaches. The first two aforementioned means of mitigation of pollutants need high operational costs and are prone to secondary pollution that necessitates integrated pollution management to reduce and/or remove the toxic pollutants from the environment [5,24]. On the other hand, the biological method (bioremediation) is another promising technology that is prominent, eco-friendly, cost-effective, efficient and easily applicable for the treatment of hydrocarbon-contaminated environments [1,10,14,19,26,28,39,44,47] but it possibly requires a long period of time for complete degrading of pollutants [18].…”

Occurrence of hydrocarbon-utilising bacteria in oil-polluted arid soils in Sudan

“…Unavoidable daily contamination has been the result of a rise of gasoline/diesel pumping station due to an increase in the number of vehicles in Khartoum and other cities. Spills of oil during tanker transfer, and leakage from underground storage tanks are the cause of groundwater contamination (Rahman et al, 2002;Limaa et al, 2019).…”