Isolation and Characterization of Human Amniotic Mesenchymal Stem Cells and Their Chondrogenic Differentiation

Nogami, Makiko; Tsuno, Hiroaki; Koike, Chika; Okabe, Motonori; Yoshida, Toshiko; Seki, Shoji; Matsui, Yoshito; Kimura, Takeshi; Nikaido, Toshio

doi:10.1097/tp.0b013e3182529b76

Cited by 52 publications

(31 citation statements)

References 31 publications

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“…The results demonstrated that various factors were able to induce the ADSCs to differentiate into different directions, and that the ADSCs derived from mesoderm were able to differentiate into endodermal and ectodermal cells. The autologous features of these stem cells, in combination with their distinct pluripotency and easy acquirement, make ADSCs an attractive choice for future tissue engineering and cell-based therapies (28–30). …”

Section: Discussionmentioning

confidence: 99%

In�vitro culture and biological properties of broiler adipose‑derived stem cells

Pei

Wang

et al. 2018

Exp Ther Med

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In the past 10 years, adipose-derived stem cells (ADSCs) have been applied due to their pluripotency. Experimental tissues have been frequently obtained from mammals, including rabbits, mice and humans, but rarely from broilers, Gallus gallus domesticus. In the present study, ADSCs were obtained from 20-day-old broiler embryos. Primary ADSCs were sub-cultured to passage 37 in vitro. The surface markers of ADSCs, namely CD29, CD31, CD44, CD71 and CD73, were detected by reverse transcription polymerase chain reaction and immunofluorescence assays. The result indicated that CD29, CD44, CD71 and CD73 were expressed on the surface of cells at various passages, but not CD31. The growth curve of cells at the different passages had a typical sigmoidal shape. Furthermore, ADSCs were successfully induced to differentiate into osteoblasts, adipocytes and hepatocyte-like cells. The results denote that the ADSCs isolated from broilers have similar biological properties to those of ADSCs obtained from other animals. The present study provided a theoretical and experimental foundation for the use of poultry as a source of stem cells, and laid a foundation for adipose tissue engineering and strategies in regenerative medicine.

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Section: Discussionmentioning

confidence: 99%

In�vitro culture and biological properties of broiler adipose‑derived stem cells

Pei

Wang

et al. 2018

Exp Ther Med

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“…Human amniotic epithelial cells are derived from the epiblast, and human amniotic mesenchymal stromal cells are derived from the hypoblast. [26][27][28] Mesenchymal stem cells may modulate the immune response. The T lymphocyte is a major effector of the adaptive immune response, and mesenchymal stem cells may modulate the function of T cells.…”

Section: Discussionmentioning

confidence: 99%

Untitled

2014

Exp Clin Transplant

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“…Cells were stained by incubating with antibodies of mesenchymal stem cell markers (anti-human CD90-FITC, and anti-human CD105-Alexa647; BD Biosciences) 14,15 for 30 min. Primary antibodies were diluted according to the manufacturer's instructions.…”

Section: Facs Analysesmentioning

confidence: 99%

Identification of Human Juvenile Chondrocyte-Specific Factors that Stimulate Stem Cell Growth

Taylor

Lee

Smeriglio

et al. 2016

Tissue Engineering Part A

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Although regeneration of human cartilage is inherently inefficient, age is an important risk factor for osteoarthritis. Recent reports have provided compelling evidence that juvenile chondrocytes (from donors below 13 years of age) are more efficient at generating articular cartilage as compared to adult chondrocytes. However, the molecular basis for such a superior regenerative capability is not understood. To identify the cell-intrinsic differences between juvenile and adult cartilage, we have systematically profiled global gene expression changes between a small cohort of human neonatal/juvenile and adult chondrocytes. No such study is available for human chondrocytes although young and old bovine and equine cartilage have been recently profiled. Our studies have identified and validated new factors enriched in juvenile chondrocytes as compared to adult chondrocytes including secreted extracellular matrix factors chordin-like 1 (CHRDL1) and microfibrillar-associated protein 4 (MFAP4). Network analyses identified cartilage development pathways, epithelial-mesenchymal transition, and innate immunity pathways to be overrepresented in juvenile-enriched genes. Finally, CHRDL1 was observed to aid the proliferation and survival of bone marrow-derived human mesenchymal stem cells (hMSC) while maintaining their stem cell potential. These studies, therefore, provide a mechanism for how young cartilage factors can potentially enhance stem cell function in cartilage repair.

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Isolation and Characterization of Human Amniotic Mesenchymal Stem Cells and Their Chondrogenic Differentiation

Abstract: These results suggest that HAMα cells correspond to MSCs that are highly proliferative and multipotent. Their chondrogenic potential and low immunogenicity indicate that HAMα cells could be an allotransplantable cell resource for cartilage repair.

Cited by 52 publications

References 31 publications

In�vitro culture and biological properties of broiler adipose‑derived stem cells

In�vitro culture and biological properties of broiler adipose‑derived stem cells

Untitled

Identification of Human Juvenile Chondrocyte-Specific Factors that Stimulate Stem Cell Growth

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