Isolation and characterization of human gastric cell lines with stem cell phenotypes

Yang, Yuan-Chieh; Wang, Shengwen; Hung, Hsiao-Yen; Chang, Chia‐Cheng; Wu, I-Chen; Huang, Yeou‐Lih; Lin, Tsai-Ming; Tsai, Jin-Lian; Chen, Angela; Kuo, Fu‐Chen; Wang, Wenming; Wu, Deng‐Chyang

doi:10.1111/j.1440-1746.2007.05031.x

Cited by 44 publications

(40 citation statements)

References 32 publications

(66 reference statements)

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“…The results showed that CS12 were more resistant to 5-fluorouracil than CSN cells (Figure 1E). Their in vivo tumorigenicity was examined using a xenograft transplantation test and only CS12 cells generated tumors in SCID mice (Figure 1F) consistent with previous findings [30, 31]. The tumor showed 10% malignant cells that contained little cytoplasm (Supplementary Figure 1B).…”

Section: Resultssupporting

confidence: 85%

“…Both CS12 and CSN cells express OCT4 stemness genes [30, 31]. Here, we further characterized whether CS12 possessed typical stem cell features.…”

Section: Resultsmentioning

confidence: 99%

“…Human gastric normal cells CSN and cancer cells CS12 cells were cultured as described elsewhere [30] with a slight modification to include Keratinocyte-SFM (Gibco-Invitrogen Co., Carlsbad, CA, USA). 293T cells were obtained from the RIKEN Cell Bank (Tsukuba, Ibaraki, Japan) and were cultured in Dulbecco's modified Eagle's minimal essential medium (DMEM) (Gibco) supplemented with 10% charcoal-stripped FBS (Gibco) with or without 1% penicillin and streptomycin (Gibco).…”

Section: Methodsmentioning

confidence: 99%

“…We have previously established a nonmalignant gastric cell line, CSN, from the stomach mucosa of a patient with mild gastritis, which exhibits features of stem/progenitor cells [30]. After a prolong expansion of CSN cells, a tumorigenic subline CS12 was generated, which exhibited anchorage-independent growth, xenograft tumor formation in nude mice, duplication of the short arm of chromosome 7 (7p15.1–15.3 and 7p22.1–22.3) on chromosome 12, and increased expression of HoxA cluster genes when compared with the nontumorigenic CSN cells [31].…”

Section: Introductionmentioning

confidence: 99%

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Oncogenic function of the homeobox A13-long noncoding RNA HOTTIP-insulin growth factor-binding protein 3 axis in human gastric cancer

et al. 2016

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To study the mechanisms of gastric tumorigenesis, we have established CSN cell line from human normal gastric mucosa, and CS12, a tumorigenic and invasive gastric cancer cell line from CSN passages. Many stem cell markers were expressed in both CSN and CS12 cells, but LGR5 and NANOG were expressed only in CS12 cells. Increased expression of homeobox A13 (HoxA13) and its downstream cascades was significant for the tumorigenic activity of CS12 cells, and was associated with recruitment of E2F-1 to HoxA13 promoter accompanied with increased trimethylation of histone H3 lysine 4 (H3K4me3) at the hypomethylated E2F motifs. Knockdown of HoxA13 caused the downregulation of long non-coding RNA HOTTIP and insulin growth factor-binding protein 3 (IGFBP-3) genes, indicating that both were targets of HoxA13. Concurrent regulation of HoxA13-HOTTIP was mediated by the mixed lineage leukemia-WD repeat domain 5 complex, which caused the trimethylation of H3K4 and then stimulated cell proliferation. HoxA13 transactivated the IGFBP-3 promoter through the HOX-binding site. Activation of IGFBP-3 stimulated the oncogenic potential and invasion activity. Increased expression of HoxA13 (63.2%) and IGFBP-3 (28.6%) was detected in human gastric cancer tissues and was found in the gastric cancer data of The Cancer Genome Atlas. Taken together, the HoxA13–HOTTIP–IGFBP-3 cascade is critical for the carcinogenic characteristics of CS12 cells.

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Section: Resultssupporting

confidence: 85%

“…Both CS12 and CSN cells express OCT4 stemness genes [30, 31]. Here, we further characterized whether CS12 possessed typical stem cell features.…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Oncogenic function of the homeobox A13-long noncoding RNA HOTTIP-insulin growth factor-binding protein 3 axis in human gastric cancer

et al. 2016

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“…Growth factors, hormones, extracellular matrix, and cytokines can also block GJIC [38]. In many AS cells, neither the connexin genes are expressed nor are the gap junctions functional [39]. The cancer cells completely lose expression of connexin gene.…”

Section: Fig 5: Es Cell Derived Differentiation-related Endpoints Usmentioning

confidence: 99%

Mesenchymal Stem Cells: An Innovative Approach in Pharmacokinetics

Tripathy¹,

Mohanty²

2017

Asian J Pharm Clin Res

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Multipotent mesenchymal stem cells (MSCs) are special kind of stem cells which originate from mesenchyme. These cells can be used as an imperative tool to study reproductive toxicity, carcinogenicity, mutagenicity, genotoxicity, and pharmacokinetics. This novel system may reveal toxicantinduced etiology, decipher detailed understanding on molecular mechanisms of toxicants induced pathways and also enumerate the safe dose of an investigational product. Hence, this could ultimately replace, improve or overtake current predictive models in toxicology. The particular review describes the utilization of MSCs in different field of toxicological and pharmacological research.

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Formation of solid tumors by a single multinucleated cancer cell

Zhang

Ramoth

et al. 2011

Cancer

137

116

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BACKGROUND: Large multinucleated cells (MNCs) commonly exist in tumorigenic cancer cell lines that are used widely in research. However, the contributions of MNCs to tumorigenesis are unknown. METHODS: In this study, MNCs were characterized in the murine fibrosarcoma cell line UV-2237 in vitro and in vivo at the single-cell level. RESULTS: The authors observed that MNCs originated from a rare subpopulation of mononuclear cells and were positive for a senescent marker, b-galactosidase. In addition, MNCs were responsible for the majority of clonogenic activity when cultured in hard agar; they were more resistant to chemotherapeutic agents than mononuclear cells; they could undergo asymmetric division (producing mononuclear cells) and self-renewal in vitro and in vivo; and, most important; a single MNC produced orthotopic, subcutaneous tumors (composed mainly of mononuclear cells) that gave rise to spontaneous lung metastases in nude mice. CONCLUSIONS: The current results indicated that the growth of MNCs may be arrested under stress and that MNCs are highly resistant to chemotherapy and can generate clonal, orthotopic, metastatic tumors. Cancer 2011;117:4092-

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Isolation and characterization of human gastric cell lines with stem cell phenotypes

Cited by 44 publications

References 32 publications

Oncogenic function of the homeobox A13-long noncoding RNA HOTTIP-insulin growth factor-binding protein 3 axis in human gastric cancer

Oncogenic function of the homeobox A13-long noncoding RNA HOTTIP-insulin growth factor-binding protein 3 axis in human gastric cancer

Mesenchymal Stem Cells: An Innovative Approach in Pharmacokinetics

Formation of solid tumors by a single multinucleated cancer cell

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