Isolation and Characterization of Human Clonogenic Osteoblast Progenitors Immunoselected from Fetal Bone Marrow Stroma Using STRO-1 Monoclonal Antibody

Oyajobi, Babatunde O.; Lomri, Abderrahim; Hott, M.; Marie, Pierre J.

doi:10.1359/jbmr.1999.14.3.351

Cited by 81 publications

(53 citation statements)

References 70 publications

(218 reference statements)

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“…On the contrary, the late differentiation marker, osteocalcin (OC, Figure 6b), showed a significantly higher value only after 21 days. Normally, the alkaline phosphatase activity and type I collagen synthesis should be higher at earlier times and osteocalcin synthesis at later times 12,20 . This discrepancy could be explained by decreased cell viability after 14 days.…”

Section: Resultsmentioning

confidence: 99%

“…Immortalized human stromal cells (STRO-1A) were graciously provided by Marie et al 20 . When STRO-1A cells had reached confluence, the cells were harvested, detached with trypsin-ethylenediamine tetra-acetic acid (EDTA; Sigma-Aldrich, UK), counted (5 × 10 5 cells/scaffold) and resuspended in culture medium (Iscove's medium with L-glutamine containing 10% SBF, 100 U/mL penicillin G, 100 µg/mL streptomycin sulfate and 10 -8 M dexamethasone; Sigma-Aldrich, UK).…”

Section: Cell Culture With Osteoprogenitor Stromal Cellsmentioning

confidence: 99%

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In Vitro Biological Evaluation of 3-D Hydroxyapatite/Collagen (50/50 wt. (%)) Scaffolds

2011

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Hydroxyapatite-collagen (HA/Col) composites are potential scaffolds for bone tissue engineering. In this work, three-dimensional (3-D) HA/Col (50/50 wt. (%)) scaffolds were synthesized using a selfassembly method and cross-linked with a 0.125% glutaraldehyde solution. Scaffolds were evaluated in vitro by cytotoxicity testing using MC3T3 cells; proliferation and differentiation were studied using STRO-1A human stromal cells for up to 21 days. Morphological and histological examinations showed a fibrous structure with a good distribution and homogeneous HA particles distribution. By thermogravimetric analysis, a ratio of 1.2 between inorganic and organic phase was found. The scaffolds presented no cytotoxicity when evaluated using three different parameters of cell survival and integrity: 2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT), Neutral Red (NR) and Crystal Violet Dye Elution (CVDE). STRO-1A cells were found to adhere, proliferate and differentiate on the 3-D scaffold, but limited cell penetration was observed.

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Section: Resultsmentioning

confidence: 99%

Section: Cell Culture With Osteoprogenitor Stromal Cellsmentioning

confidence: 99%

In Vitro Biological Evaluation of 3-D Hydroxyapatite/Collagen (50/50 wt. (%)) Scaffolds

2011

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“…Additionally, we used human clonal pluripotent bone marrow stromal cells immunoselected with the murine IgM monoclonal antibody STRO-1, a cell surface antigen expressed by stromal elements in human bone marrow [39]. The selected cells (F/STRO-1 þ A) show osteogenic differentiation potential under appropriate stimulation [2,40]. Cells were routinely cultured in Dulbecco's modified Eagle's medium (DMEM, Invitrogen, Paisley, Scotland, http://www.invitrogen.com) supplemented with 10% heat inactivated fetal calf serum, 1% L-glutamine, and penicillin/streptomycin (10,000 U/mL and 10,000 lg/mL, respectively) at 37 C in humidified atmosphere containing 5% CO 2 in air.…”

Section: Cells Culturesmentioning

confidence: 99%

“…Human and murine mesenchymal stromal cells (MSC) derived from the bone marrow stroma have the ability to differentiate into chondroblasts, osteoblasts, or adipocytes under appropriate stimulation [1][2][3][4]. The differentiation potential of these cells makes them an appropriate source for cell engineering and bone matrix regeneration [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Promotion of Osteoblast Differentiation in Mesenchymal Cells Through Cbl-Mediated Control of STAT5 Activity

et al. 2013

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The identification of the molecular mechanisms controlling the degradation of regulatory proteins in mesenchymal stromal cells (MSC) may provide clues to promote MSC osteogenic differentiation and bone regeneration. Ubiquitin ligase-dependent degradation of proteins is an important process governing cell fate. In this study, we investigated the role of the E3 ubiquitin ligase c-Cbl in MSC osteoblast differentiation and identified the mechanisms involved in this effect. Using distinct shRNA targeting c-Cbl, we showed that c-Cbl silencing promotes osteoblast differentiation in murine and human MSC, as demonstrated by increased alkaline phosphatase activity, expression of phenotypic osteoblast marker genes (RUNX2, ALP, type 1 collagen), and matrix mineralization in vitro. Coimmunoprecipitation analyses showed that c-Cbl interacts with the transcription factor STAT5, and that STAT5 forms a complex with RUNX2, a master transcription factor controlling osteoblastogenesis. Silencing c-Cbl decreased c-Cblmediated STAT5 ubiquitination, increased STAT5 protein level and phosphorylation, and enhanced STAT5 and RUNX2 transcriptional activity. The expression of insulin like growth factor-1 (IGF-1), a target gene of STAT5, was increased by c-Cbl silencing in MSC and in bone marrow stromal cells isolated from c-Cbl deficient mice, suggesting that IGF-1 contributes to osteoblast differentiation induced by c-Cbl silencing in MSC. Consistent with these findings, pharmacological inhibition of STAT5 activity, or neutralization of IGF-1 activity, abrogated the positive effect of c-Cbl knockdown on MSC osteogenic differentiation. Taken together, the data provide a novel functional mechanism by which the ubiquitin ligase c-Cbl regulates the osteoblastic differentiation program in mesenchymal cells by controlling Cbl-mediated STAT5 degradation and activity.

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“…STRO1 + MSC can differentiate into multiple mesenchymal lineages, including hematopoiesis-supportive stromal cells, pericytes, adipocytes, myofibroblasts, myocytes, cardiomyocytes, osteoblasts, chondrocytes, and neurons [48,62,131,139,186]. The MSC surface markers CD146 and CD105 are also expressed on endothelial precursor cells, which possess characteristics similar to those of pericytes [17,169].…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

Cancer stem cells as targets for cancer therapy: selected cancers as examples

Hombach‐Klonisch

Paranjothy

Wiecheć

et al. 2008

Arch. Immunol. Ther. Exp.

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It is becoming increasingly evident that cancer constitutes a group of diseases involving altered stem-cell maturation/differentiation and the disturbance of regenerative processes. The observed malignant transformation is merely a symptom of normal differentiation processes gone astray rather than the primary event. This review focuses on the role of cancer stem cells (CSCs) in three common but also relatively under-investigated cancers: head and neck, ovarian, and testicular cancer. For didactic purpose, the physiology of stem cells is first introduced using hematopoietic and mesenchymal stem cells as examples. This is followed by a discussion of the (possible) role of CSCs in head and neck, ovarian, and testicular cancer. Aside from basic information about the pathophysiology of these cancers, current research results focused on the discovery of molecular markers specific to these cancers are also discussed. The last part of the review is largely dedicated to signaling pathways active within various normal and CSC types (e.g. Nanog, Nestin, Notch1, Notch2, Oct3 and 4, Wnt). Different elements of these pathways are also discussed in the context

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Isolation and Characterization of Human Clonogenic Osteoblast Progenitors Immunoselected from Fetal Bone Marrow Stroma Using STRO-1 Monoclonal Antibody

Abstract: ABSTRACT

Cited by 81 publications

References 70 publications

In Vitro Biological Evaluation of 3-D Hydroxyapatite/Collagen (50/50 wt. (%)) Scaffolds

In Vitro Biological Evaluation of 3-D Hydroxyapatite/Collagen (50/50 wt. (%)) Scaffolds

Promotion of Osteoblast Differentiation in Mesenchymal Cells Through Cbl-Mediated Control of STAT5 Activity

Cancer stem cells as targets for cancer therapy: selected cancers as examples

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