Isolation and characterization of heparin and gelatin binding buffalo seminal plasma proteins and their effect on cauda epididymal spermatozoa

Arunachalam, A.; Singh, L. P.; Ahmed, Nizamuddin; Ansari, M.R.; Ram, G. C.

doi:10.1016/j.anireprosci.2004.12.014

Cited by 46 publications

(38 citation statements)

References 20 publications

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“…In the current study, this was not attributed to changes in the motility or velocity of epididymal spermatozoa. The means by which seminal plasma confers this effect is yet to be established in sheep, but results from other species have demonstrated that individual seminal plasma proteins increase sperm penetration of cervical mucus (heparin-and gelatinebinding proteins in buffalos (Arangasamy et al 2005) and glycoprotein b defensin 126 in macaques (Tollner et al 2008)) possibly through modification of the negative net charge of the sperm membrane (Tollner et al 2008). Clearly, considerable further research is required to investigate individual proteins within ram seminal plasma that may promote mucus penetration, not to mention the nature of the sperm-mucus interaction itself.…”

Section: Cervicalmentioning

confidence: 99%

“…Similarly, through the use of sperm penetration tests, seminal plasma and its individual proteins have been shown to enhance the ability of human (Overstreet et al 1980), ram (Maxwell et al 1999), buffalo (Arangasamy et al 2005) and macaque (Tollner et al 2008) spermatozoa to migrate through cervical mucus. While not definitive, these results suggest that seminal plasma may play a role in the migration of ram spermatozoa through the cervix.…”

Section: Introductionmentioning

confidence: 99%

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Seminal plasma aids the survival and cervical transit of epididymal ram spermatozoa

Rickard¹,

Pini²,

Soleilhavoup³

et al. 2014

Reproduction

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Seminal plasma purportedly plays a critical role in reproduction, but epididymal spermatozoa are capable of fertilisation following deposition in the uterus, calling into question the biological requirement of this substance. Through a combination of direct observation of spermatozoa in utero using probe-based Confocal Laser Endomicroscopy, in vivo assessment of sperm fertility and in vitro analysis of various sperm functional parameters, this study investigated the role of seminal plasma in spermatozoa transit through the cervix of the ewe. Following deposition in the cervical os, epididymal spermatozoa previously exposed to seminal plasma displayed an enhanced ability to traverse the cervix as evidenced by both significantly higher pregnancy rates and numbers of spermatozoa observed at the utero-tubal junction when compared with epididymal spermatozoa not previously exposed to seminal plasma. The beneficial effect of seminal plasma on sperm transport was clearly localised to transit through the cervix as pregnancy rates of spermatozoa deposited directly into the uterus were unaffected by exposure to seminal plasma. This phenomenon was not explained by changes to sperm motion characteristics, as seminal plasma had no effect on the motility, kinematic parameters or mitochondrial membrane potential of spermatozoa. Rather, in vitro testing revealed that seminal plasma improved the ability of epididymal spermatozoa to penetrate cervical mucus recovered from ewes in oestrus. These results demonstrate that the survival and transport of ram spermatozoa through the cervix of the ewe is not linked to their motility or velocity but rather the presence of some cervical penetration trait conferred by exposure to seminal plasma.

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Section: Cervicalmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Seminal plasma aids the survival and cervical transit of epididymal ram spermatozoa

Rickard¹,

Pini²,

Soleilhavoup³

et al. 2014

Reproduction

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“…the half time for BSP1 binding to sperm is calculated as !1 s (Mü ller et al 1998). Homologs of bovine BSP proteins have been identified in several other mammalian species including humans ), buffalo (Arangasamy et al 2005), bison (Boisvert et al 2004), boar, stallions (Calvete et al 1997), rams (Barrios et al 2005, Bergeron et al 2005, goats (Villemure et al 2003), and mice (Plante et al 2012). In cattle, three BSP proteins, BSP1 (previously known as PDC-109), BSP3 (BSP-A3), and BSP5 (BSP-30 kDa), have been found to play a role in fertilization ).…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation increases coating of bull sperm by seminal plasma binder of sperm proteins BSP1, BSP3, and BSP5

Ardón

Suárez

2013

Reproduction

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Artificial insemination with frozen semen allows affordable, worldwide dissemination of gametes with superior genetics. Nevertheless, sperm are damaged by the cryopreservation process. Elucidating the molecular effects of cryopreservation on sperm could suggest methods for improving fertility of frozen/thawed semen. This study was undertaken to examine the effect of cryopreservation on the coating of sperm by binder of sperm (BSP) proteins in seminal plasma. BSP proteins are secreted by the seminal vesicles and coat the surface of sperm by partially intercalating into the outer leaflet of the sperm plasma membrane. The BSP proteins are known to play roles in the formation of the oviductal sperm storage reservoir and in sperm capacitation. We investigated the effects of cryopreservation on the sperm BSP protein coat using Bovipure to separate live sperm from extended semen and then assaying the amounts of BSP proteins on sperm using quantitative western blotting with custom-made antibodies against unique sequences of each BSP protein. Greater amounts of all three BSP proteins (BSP1, BSP3, and BSP5) were detected on frozen/thawed sperm than on fresh sperm. Furthermore, the reduction of BSP3 from 15 to 13 kDa in mass, which occurs during incubation of sperm under mild capacitating conditions, was enhanced by cryopreservation. We concluded that freezing alters the BSP protein coating on sperm, which could account in part for reduced fertility of cryopreserved semen samples.

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“…Presence of HBPs in sperm membranes was indicative of the fertility potential of bulls [12]. HBPs modulate capacitation and zona binding ability of buffalo cauda epididymal spermatozoa [13]. The total seminal plasma protein (TSPP) concentration in buffalo bulls was ranged from 28 to 36 mg/mL [14,15] and concentration of buffalo HBPs reported in the range from 1.47 to 2.61 mg/mL [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

“…The total seminal plasma protein (TSPP) concentration in buffalo bulls was ranged from 28 to 36 mg/mL [14,15] and concentration of buffalo HBPs reported in the range from 1.47 to 2.61 mg/mL [15][16][17]. Eight major buffalo HBPs were reported in the molecular weight range of 13-71 kDa [13]. Until date, the study on buffalo HBPs in terms of their concentration and numbers in relation with initial semen quality is meager.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of heparin-binding proteins profile of Murrah buffalo (Bubalus bubalis) semen

Ramteke¹,

Shende²,

Rajoriya³

et al. 2014

Vet World

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Aim:The experiment was conducted to study the total seminal plasma protein (TSPP) and heparin-binding proteins (HBPs) in relation to initial semen quality of buffalo bull. Materials and Methods:Semen from two Murrah buffalo bulls (bull no. 605 and 790) with mass motility of ≥3+ were used for the study and categorized into three groups (Group I-Mass motility 3+, Group II-Mass motility 4+ and Group III-Mass motility 5+). Seminal plasma from semen was separated by centrifugation. HBPs was isolated and purified from heparin-agarose affinity column by modified elution buffer. TSPP and isolated HBPs concentration was estimated by Lowry's method. The purified HBPs were resolved on Sodium dodecyl sulfate polyacrylamide gel electrophoresis to check the protein profile of two bulls. Conclusion: TSPP and HBPs shows variation in concentration with respect to initial semen quality. Furthermore, presence of fertility related 31 kDa HBPs in one of the bull may be an indication of high fertility of a bull. In future, in-vivo and in-vitro correlative study on larger basis is needed for the establishment of fertility-related HBPs in semen which might establish criteria for selection of buffalo bull with high fertility potential. Results

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Isolation and characterization of heparin and gelatin binding buffalo seminal plasma proteins and their effect on cauda epididymal spermatozoa

Cited by 46 publications

References 20 publications

Seminal plasma aids the survival and cervical transit of epididymal ram spermatozoa

Seminal plasma aids the survival and cervical transit of epididymal ram spermatozoa

Cryopreservation increases coating of bull sperm by seminal plasma binder of sperm proteins BSP1, BSP3, and BSP5

Comparative study of heparin-binding proteins profile of Murrah buffalo (Bubalus bubalis) semen

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