Isolation and characterization of hematopoietic stem cells in teleost fish

Kobayashi, Isao; Katakura, Fumihiko; Moritomo, Tadaaki

doi:10.1016/j.dci.2016.01.003

Cited by 26 publications

(14 citation statements)

References 94 publications

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“…Sorted cells from HK (Figure 2A) were used as the main source of different leukocyte subpopulations for the RT-qPCR validation assays. As the major hematopoietic organ in teleost, HK consists of a heterogeneous mixture of leukocytes belonging to the lymphoid and myeloid lineages (99). HKL subsets were first examined for gene expression of known markers for B cells, macrophages, and T cells in order to confirm the nature of cells present in each subset, as well as to ensure that no cross-contamination of cell subsets occurred during FACS sorting.…”

Section: Resultsmentioning

confidence: 99%

Profiling the Atlantic Salmon IgM+ B Cell Surface Proteome: Novel Information on Teleost Fish B Cell Protein Repertoire and Identification of Potential B Cell Markers

et al. 2019

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Fish immunology research is at a pivotal point with the increasing availability of functional immunoassays and major advances in omics approaches. However, studies on fish B cells and their distinct subsets remain a challenge due to the limited availability of differentially expressed surface markers. To address this constraint, cell surface proteome of Atlantic salmon IgM+ B cells were analyzed by mass spectrometry and compared to surface proteins detected from two adherent salmon head kidney cell lines, ASK and SSP-9. Out of 21 cluster of differentiation (CD) molecules identified on salmon IgM+ B cells, CD22 and CD79A were shortlisted as potential markers based on the reported B cell-specific surface expression of their mammalian homologs. Subsequent RT-qPCR analyses of flow cytometry-sorted subpopulations from head kidney leukocytes confirmed that both cd22 and cd79a genes were highly expressed in IgM+ lymphoid cells but were observed in barely detectable levels in IgM− non-lymphoid suspension and adherent cells. Similarly, significantly high cd22 and cd79a mRNA levels were observed in IgM+ or IgT+ lymphoid cells from the spleen and peritoneal cavity, but not in their corresponding IgM− IgT− non-lymphoid fractions. This suggests that the B cell restrictive expression of CD22 and CD79A extend down to the transcription level, which was consistent across different lymphoid compartments and immunoglobulin isotypes, thus strongly supporting the potential of CD22 and CD79A as pan-B cell markers for salmon. In addition, this study provides novel information on the salmon B cell surface protein repertoire, as well as insights on B cell evolution. Further investigation of the identified salmon CD molecules, including development of immunological tools for detection, will help advance our understanding of the dynamics of salmon B cell responses such as during infection, vaccination, or immunostimulation.

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Section: Resultsmentioning

confidence: 99%

Profiling the Atlantic Salmon IgM+ B Cell Surface Proteome: Novel Information on Teleost Fish B Cell Protein Repertoire and Identification of Potential B Cell Markers

et al. 2019

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“…The precursor or parent cells for the cells mentioned above and for lymphoid cells are haematopoietic stem cells (HSCs) and haematopoietic progenitor cells (HPCs) (14). In Osteichthyes, these types of cells develop in the hind kidney ( mesonephros ), a part of an organ having an excretory function where the key role in the formation of cells of this subpopulation is played by renal tubules (6, 19, 20, 21). In Osteichthyes, thrombocytes take part in haemostatic processes, including aggregation and release of endothrombocytic granule components in cases of blood vessel damage, and in the immune response development as part of innate and adaptive immune mechanisms (4, 5, 7, 24, 25, 36, 41).…”

Section: Introductionmentioning

confidence: 99%

Characterisation of thrombocytes in Osteichthyes

Stosik

Tokarz–Deptuła²,

Deptuła

2019

Journal of Veterinary Research

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Thrombocytes in vertebrates other than mammals, inter alia in fish, are analogues of platelets in mammals. In Osteichthyes, these cells take part in haemostatic processes, including aggregation and release reactions in cases of blood vessel damage, and in the immune response development as well. This paper discusses the development of thrombocytes in Osteichthyes, taking into account the need to make changes to the concept of grouping progenitor cells as suggested in the literature. The following pages present the morphological and cytochemical properties of thrombocytes as well as their defence functions, and also point out differences between thrombocytes in fish and platelets in mammals. The paper further highlights the level of thrombocytes’ immune activity observed in fish and based on an increased proportion of these cells in response to antigenic stimulation, on morphological shifts towards forms characteristic of dendritic cells after antigenic stimulation and on the presence of surface structures and cytokines released through, inter alia, gene expression of TLR receptors, MHC class II protein-coding genes and pro-inflammatory cytokines. The study also points out the need to recognise thrombocytes in Osteichthyes as specialised immune cells conditioning non-specific immune mechanisms and playing an important role in affecting adaptive immune mechanisms.

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“…All haematopoietic differentiation pathways, i.e. erythropoiesis, myelopoiesis, and lymphopoiesis, have been identified in these fish [11, 12, 14]. The highest concentration of developmental forms of B cells conditioning specific humoral immunity is observed in the anterior kidney.…”

Section: Haematopoiesis B Cells Plasmablasts and Plasma Cellsmentioning

confidence: 99%

Specific humoral immunity in Osteichthyes

Stosik¹,

Tokarz–Deptuła²,

Deptuła³

2018

cejoi

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The fish immune system is extremely complex and has considerable adaptive potential. In Osteichthyes, the system is formed by lymphopoietic organs which are important for the differentiation and maturation of the immune system cells. These organs include the anterior kidney (phronephros), the thymus, the spleen, the posterior kidney (mesonephros), and mucosa-associated lymphoid tissues (MALT). Apart from the lymphocytic organs and the MALT system, the immune system components include defensive cells and their products. Those identified in fish include, inter alia, monocytes/macrophages, melanomacrophages, neutrophilic granulocytes, thrombocytes, B cells, plasma cells, and T cells. The roles of the individual components of the organisation of the immune system, the organs, and lymphoid tissue as well as the constituents conditioning the innate and adaptive immunity mechanisms are considered equally important, especially in the context of functional interdependence. The progress in the exploration of the processes of specific humoral immunity in Osteichthyes and the possibilities of their practical application is increasingly promising in view of the expected need for protection of fish against diseases.The paper discusses selected issues concerning recent knowledge about haematopoiesis of B cells, plasmablasts, plasma cells, and immunoglobulins (IgM, IgD, IgT/IgZ).

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Isolation and characterization of hematopoietic stem cells in teleost fish

Cited by 26 publications

References 94 publications

Profiling the Atlantic Salmon IgM+ B Cell Surface Proteome: Novel Information on Teleost Fish B Cell Protein Repertoire and Identification of Potential B Cell Markers

Profiling the Atlantic Salmon IgM+ B Cell Surface Proteome: Novel Information on Teleost Fish B Cell Protein Repertoire and Identification of Potential B Cell Markers

Characterisation of thrombocytes in Osteichthyes

Specific humoral immunity in Osteichthyes

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