Isolation and Characterization of Halotolerant Alkaliphilic Methanotrophic Bacteria from Tuva Soda Lakes

Khmelenina, V. N.; Kalyuzhnaya, Marina G.; Starostina, Natalia G.; Сузина, Н. Е.; Trotsenko, Yu. A.

doi:10.1007/s002849900249

Cited by 136 publications

(76 citation statements)

References 17 publications

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“…Interestingly, the haloalkaliphilic/-tolerant Methylomicrobium isolates were resistant to heat and desiccation despite the absence of cysts (Kaluzhnaya et al, 2001). Protection from such extreme conditions is mediated by the intracellular accumulation of the compatible solute ectoine (Khmelenina et al, 1997Trotsenko et al, 2005). Methane-based DNA-SIP experiments with Transbaikal soda lake sediments identified the dominant methanotrophs as Methylomicrobium spp.…”

Section: Discussionmentioning

confidence: 99%

“…Methylomicrobium spp. have been isolated from several terrestrial and marine samples (Bowman et al, 1993(Bowman et al, , 1995Sieburth et al, 1987;Fuse et al, 1998) and soda lake sediments (Kalyuzhnaya et al, 1999(Kalyuzhnaya et al, , 2008Khmelenina et al, 1997Khmelenina et al, , 2000Sorokin et al, 2000;Kaluzhnaya et al, 2001). Interestingly, the haloalkaliphilic/-tolerant Methylomicrobium isolates were resistant to heat and desiccation despite the absence of cysts (Kaluzhnaya et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

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Active methylotrophs in the sediments of Lonar Lake, a saline and alkaline ecosystem formed by meteor impact

et al. 2010

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Lonar Lake is a unique saline and alkaline ecosystem formed by meteor impact in the Deccan basalts in India around 52 000 years ago. To investigate the role of methylotrophy in the cycling of carbon in this unusual environment, stable-isotope probing (SIP) was carried out using the onecarbon compounds methane, methanol and methylamine. Denaturing gradient gel electrophoresis fingerprinting analyses performed with heavy 13 C-labelled DNA retrieved from sediment microcosms confirmed the enrichment and labelling of active methylotrophic communities. Clone libraries were constructed using PCR primers targeting 16S rRNA genes and functional genes. Methylomicrobium, Methylophaga and Bacillus spp. were identified as the predominant active methylotrophs in methane, methanol and methylamine SIP microcosms, respectively. Absence of mauA gene amplification in the methylamine SIP heavy fraction also indicated that methylamine metabolism in Lonar Lake sediments may not be mediated by the methylamine dehydrogenase enzyme pathway. Many gene sequences retrieved in this study were not affiliated with extant methanotrophs or methylotrophs. These sequences may represent hitherto uncharacterized novel methylotrophs or heterotrophic organisms that may have been cross-feeding on methylotrophic metabolites or biomass. This study represents an essential first step towards understanding the relevance of methylotrophy in the soda lake sediments of an unusual impact crater structure.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Active methylotrophs in the sediments of Lonar Lake, a saline and alkaline ecosystem formed by meteor impact

et al. 2010

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“…Preparation of thin sections and electron microscopy were done as described previously (Khmelenina et al, 1997(Khmelenina et al, , 1999. For freeze-fractured preparations, exponentially growing cells were collected and frozen in liquid propane cooled to k196 mC with liquid nitrogen.…”

Section: Verification Of Purity Of Strain B2mentioning

confidence: 99%

Methylocapsa acidiphila gen. nov., sp. nov., a novel methane-oxidizing and dinitrogen-fixing acidophilic bacterium from Sphagnum bog.

Dedysh¹,

Khmelenina²,

Suzina³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

244

212

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“…Alkaliphiles consist of two main physiological groups of microorganisms; alkaliphiles and haloalkaliphiles (Khmelenina et al, 1997;Mokashe et al, 2015). Members of the former group require alkaline pH of 9 or more for their growth, while those of the latter require an alkaline pH (pH 9) in addition to high salinity.…”

Section: Introductionmentioning

confidence: 99%

“…Alkaliphiles have clearly gained large amounts of genetic information by evolutionary processes and exhibit an ability in their genes to cope with particular environments; therefore their genes are a potentially valuable source of information waiting to be explored and exploited by the biotechnologists (Khmelenina et al, 1997;Mokashe et al, 2015). Alkaliphiles consist of two main physiological groups of microorganisms; alkaliphiles and haloalkaliphiles (Khmelenina et al, 1997;Mokashe et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a haloalkaliphilic protease producer bacterium from Wadi Natrun in Egypt

Lotfy¹,

Abd-El-Karim²,

El-Sharouny³

et al. 2017

Afr. J. Biotechnol.

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A Gram-positive, rod-shaped, spore-forming haloalkaliphilic bacterium designated as NA7 was isolated from the surface of a Helianthemum nummularium root sample obtained from Wadi Natrun in Egypt. Sequence analysis of the 16S rRNA gene revealed a Bacillus haloalkaliphilius strain as the closest match with 99% identity. In a shake flask culture containing 10% NaCl, adjusted to pH 10 and incubated at 37°C, the isolated strain produced thermostable extracellular alkaline protease with relatively stable maximum activity records (0.610-0.625 TU) within a relatively long stationary phase that exceeded 60 h. A 2-level fractional factorial design (Plackett-Burman) was then applied to screen for nutritional and cultivation factors regulating protease production by the isolate and to appraise their effects. Calculated statistical parameters revealed that NaCl and MgSO 4 are the most significant independent variables affecting alkaline protease production by NA7 and suggested a near-optimum culture condition. Verification of this predicted condition resulted in an alkaline protease specific activity record of 509 TU/mg protein with a 1.27 fold increase when compared to the basal medium culture.

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Isolation and Characterization of Halotolerant Alkaliphilic Methanotrophic Bacteria from Tuva Soda Lakes

Cited by 136 publications

References 17 publications

Active methylotrophs in the sediments of Lonar Lake, a saline and alkaline ecosystem formed by meteor impact

Active methylotrophs in the sediments of Lonar Lake, a saline and alkaline ecosystem formed by meteor impact

Methylocapsa acidiphila gen. nov., sp. nov., a novel methane-oxidizing and dinitrogen-fixing acidophilic bacterium from Sphagnum bog.

Isolation and characterization of a haloalkaliphilic protease producer bacterium from Wadi Natrun in Egypt

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