Isolation and characterization of galactomannans from Prosopis affinis as potential gum substitutes

Vilaró, Pilar; Bennadji, Zohra; Budelli, Eliana; Moyna, Guillermo; Panizzolo, Luis Alberto; Ferreira, Fernando

doi:10.1016/j.foodhyd.2017.10.038

Cited by 30 publications

(9 citation statements)

References 35 publications

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“…Purified galactomannans extracted from P. affinis at room temperature (GMP 1/RT ) with M/G at a ratio of 1.6, an average molar mass of 1.6 × 10 6 g∙mol −1 (determined by SEC) [ 24 ], and a PDI of 0.3 (determined by DLS) were used. Trimethylsilyl cellulose (TMSC, Avicel, M w = 185,000 g·mol −1 , M n = 30,400 g·mol −1 , PDI = 6.1 determined by GPC in chloroform) with a DS Si value of 2.8 was purchased from TITK (Rudolstadt, Germany).…”

Section: Methodsmentioning

confidence: 99%

Interactions and Dissociation Constants of Galactomannan Rendered Cellulose Films with Concavalin A by SPR Spectroscopy

et al. 2020

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Interactions of biomolecules at interfaces are important for a variety of physiological processes. Among these, interactions of lectins with monosaccharides have been investigated extensively in the past, while polysaccharide-lectin interactions have scarcely been investigated. Here, we explore the adsorption of galactomannans (GM) extracted from Prosopis affinis on cellulose thin films determined by a combination of multi-parameter surface plasmon resonance spectroscopy (MP-SPR) and atomic force microscopy (AFM). The galactomannan adsorbs spontaneously on the cellulose surfaces forming monolayer type coverage (0.60 ± 0.20 mg·m−2). The interaction of a lectin, Concavalin A (ConA), with these GM rendered cellulose surfaces using MP-SPR has been investigated and the dissociation constant KD (2.1 ± 0.8 × 10−8 M) was determined in a range from 3.4 to 27.3 nM. The experiments revealed that the galactose side chains as well as the mannose reducing end of the GM are weakly interacting with the active sites of the lectins, whereas these interactions are potentially amplified by hydrophobic effects between the non-ionic GM and the lectins, thereby leading to an irreversible adsorption.

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Section: Methodsmentioning

confidence: 99%

Interactions and Dissociation Constants of Galactomannan Rendered Cellulose Films with Concavalin A by SPR Spectroscopy

et al. 2020

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“…Monosaccharides composition (HPLC), molecular weight (chromatographic), galacturonic acid measurement (colorimetric method), microstructure (brightfield microscopy) [13] Insoluble DFs from Sichuan natural fermented pickle vegetables Infrared spectroscopy, TG, SEM [51] Galactomannans from Prosopis affinis HPLC, GC-MS, NMR spectroscopy, intrinsic viscosity and viscosity-average molecular weight [52] Resistant starch from Laird lentils (Lens culinaris) seeds SEC, NMR, FTIR, SEM [53] Galactomannans from Adenanthera pavonina, Caesalpinia pulcherrima, Gleditsia triacanthos and Sophora japonica seeds Monosaccharides composition, intrinsic viscosity, viscosity average molecular mass [54] Polysaccharide from Lycium barbarum L. fruit FTIR, GC-MS, NMR [29] High DF powder from lime residues SEM [55] Soluble DF from potato pulp Monosaccharide content, molecular weight distribution [56] NMR, nuclear magnetic resonance; FTIR, Fourier-transform infrared spectroscopy; AEC, anion-exchange chromatography; SEC, sizeexclusion chromatography; SEM, scanning electron microscope, GC-MS, gas chromatography-mass spectrometry; TG, thermogravimetry; HPLC, high-performance liquid chromatography.…”

Section: Polysaccharides Analysis Of Structural Characterization Referencesmentioning

confidence: 99%

Dietary Fiber: Fractionation, Characterization and Potential Sources from Defatted Oilseeds

Nevara

Muhammad

Zawawi

et al. 2021

Foods

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Dietary fiber (DF) has wide applications, especially in the food and pharmaceutical industries due to its health-promoting effects and potential techno-functional properties in developing functional food products. There is a growing interest in studies related to DF; nevertheless, there is less focus on the fractionation and characterization of DF. The characteristics of DF fractions explain their functionality in food products and provide clues to their physiological effects in food and pharmaceutical industrial applications. The review focuses on a brief introduction to DF and methods for its fractionation. It discusses the characterization of DF in terms of structural, physicochemical and rheological properties. The potential sources of DF from selected defatted oilseeds for future studies are highlighted.

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“…K. Heyne (Leguminosae) could be explored as an effective alternative to commercial galactomannans for industrial purposes [20][21][22][23][24]. Galactomannans from Prosopis affinis Spreng (Leguminosae), seeds has shown molecular weight distribution and intrinsic viscosity similar to those of commercial gums [25]. Galactomannan from fenugreek attributes depicted a very food candidacy for industrial application [26,27].…”

Section: Galactomannansmentioning

confidence: 99%

Diosgenin and galactomannans, natural products in the pharmaceutical sciences

et al. 2021

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Background Diosgenin is an isospirostane derivative, which is a steroidal sapogenin and the product of acids or enzymes hydrolysis process of dioscin and protodioscin. Galactomannans are heteropolysaccharides composed of D-mannose and D-galactose, which are major sources of locust bean, guar, tara and fenugreek. Methods Literature survey was accomplished using multiple databases including PubMed, Science Direct, ISI web of knowledge and Google Scholar. Results Four major sources of seed galactomannans are locust bean (Ceratonia siliqua), guar (Cyamopsis tetragonoloba), tara (Caesalpinia spinosa Kuntze), and fenugreek (T.foenum-graecum). Diosgenin has effect on immune system, lipid system, inflammatory and reproductive systems, caner, metabolic process, blood system, blood glucose and calcium regulation. The most important pharmacological benefits of galactomannan are antidiabetic, antioxidant, anticancer, anticholinesterase, antiviral activities, and appropriate for dengue virus and gastric diseases. Conclusions Considering the importance of diosgenin and galactomannans, the obtained findings suggest potential of diosgenin and galactomannans as natural products in pharmaceutical industries.

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Isolation and characterization of galactomannans from Prosopis affinis as potential gum substitutes

Cited by 30 publications

References 35 publications

Interactions and Dissociation Constants of Galactomannan Rendered Cellulose Films with Concavalin A by SPR Spectroscopy

Interactions and Dissociation Constants of Galactomannan Rendered Cellulose Films with Concavalin A by SPR Spectroscopy

Dietary Fiber: Fractionation, Characterization and Potential Sources from Defatted Oilseeds

Diosgenin and galactomannans, natural products in the pharmaceutical sciences

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