Isolation and characterization of four medium-size disintegrins from the venoms of Central American viperid snakes of the genera Atropoides, Bothrops, Cerrophidion and Crotalus

Angulo, Yamileth; Castro, Adriana Pinheiro de; Lomonte, Bruno; Rucavado, Alexandra; Fernández, Julián; Calvete, Juan J.; Gutiérrez, José Marı́a

doi:10.1016/j.biochi.2014.10.010

Cited by 18 publications

(10 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As has been observed for other snake venom disintegrins, including from the related Crotalus simus [24], theoretical and MALDI-MS-derived masses did not agree. It is hypothesized that this difference may result from the C-terminal truncation of tzabcanin; the mass of tzabcanin lacking the last four residues is 7098.94, which differs from tzabcanin's MALDI-determined mass by 0.08%.…”

Section: Discussioncontrasting

confidence: 41%

See 1 more Smart Citation

Disintegrins of Crotalus simus tzabcan venom: Isolation, characterization and evaluation of the cytotoxic and anti-adhesion activities of tzabcanin, a new RGD disintegrin

2015

View full text Add to dashboard Cite

Section: Discussioncontrasting

confidence: 41%

“…1) which were assessed by reduced SDS-PAGE (Fig. 1 inset) to identify proteins in the~7 kDa range, as this is the general mass of monomeric disintegrins [11,23,24,27]. Peak 6 yielded one protein band in this mass range (Fig.…”

Section: Isolation and Masses Of C S Tzabcan Disintegrins And Ntermmentioning

confidence: 99%

Disintegrins of Crotalus simus tzabcan venom: Isolation, characterization and evaluation of the cytotoxic and anti-adhesion activities of tzabcanin, a new RGD disintegrin

2015

View full text Add to dashboard Cite

“…Non-RGD disintegrin from C. d. collilineatus presents 7287.4 Da, as determined by MALDI-TOF, and it is similar to the molecular mass of others snake venom disintegrins, such as tzabcanin (7.1 kDa) [ 45 ], disintegrin from C. simus (7.1 kDa) [ 46 ] and proteomic data of C. d. collilineatus [ 39 , 40 ].…”

Section: Discussionmentioning

confidence: 78%

Cell migration inhibition activity of a non-RGD disintegrin from Crotalus durissus collilineatus venom

Oliveira

Manzini

Ferreira

et al. 2018

J Venom Anim Toxins Incl Trop Dis

View full text Add to dashboard Cite

BackgroundIn recent decades, snake venom disintegrins have received special attention due to their potential use in anticancer therapy. Disintegrins are small and cysteine-rich proteins present in snake venoms and can interact with specific integrins to inhibit their activities in cell-cell and cell-ECM interactions. These molecules, known to inhibit platelet aggregation, are also capable of interacting with certain cancer-related integrins, and may interfere in important processes involved in carcinogenesis. Therefore, disintegrin from Crotalus durissus collilineatus venom was isolated, structurally characterized and evaluated for its toxicity and ability to interfere with cell proliferation and migration in MDA-MB-231, a human breast cancer cell line.MethodsBased on previous studies, disintegrin was isolated by FPLC, through two chromatographic steps, both on reversed phase C-18 columns. The isolated disintegrin was structurally characterized by Tris-Tricine-SDS-PAGE, mass spectrometry and N-terminal sequencing. For the functional assays, MTT and wound-healing assays were performed in order to investigate cytotoxicity and effect on cell migration in vitro, respectively.ResultsDisintegrin presented a molecular mass of 7287.4 Da and its amino acid sequence shared similarity with the disintegrin domain of P-II metalloproteases. Using functional assays, the disintegrin showed low cytotoxicity (15% and 17%, at 3 and 6 μg/mL, respectively) after 24 h of incubation and in the wound-healing assay, the disintegrin (3 μg/mL) was able to significantly inhibit cell migration (24%, p < 0.05), compared to negative control.ConclusionThus, our results demonstrate that non-RGD disintegrin from C. d. collilineatus induces low cytotoxicity and inhibits migration of human breast cancer cells. Therefore, it may be a very useful molecular tool for understanding ECM-cell interaction cancer-related mechanisms involved in an important integrin family that highlights molecular aspects of tumorigenesis. Also, non-RGD disintegrin has potential to serve as an agent in anticancer therapy or adjuvant component combined with other anticancer drugs.

show abstract

“…Correlated with the possible benefic effect of proteins present in the crude venom of Egyptian cobra encouraged us to develop an analytical method for separation as much protein as possible using reverse phase HPLC. Numerous previous publication reported separation of different peptides or proteins from crude snake venom [23][24][25][26][27][28][29]. Therefore, in this study we have reported the purification of Egyptian cobra venom as well as the antibacterial activity of crude venom.…”

Section: Discussionmentioning

confidence: 86%

New RP-HPLC Method for Separation of Naja haje haje Venom and Studies of its Bactericidal Effect

Florea

Ciurca

Mare

et al. 2016

Acta Medica Marisiensis

View full text Add to dashboard Cite

Background: Snake venom is a complex mixture of biologically active substances. Some peptides and proteins from snake have already demonstrated their therapeutically potential. The venom of Naja haje, an Elapidae member, has been analyzed from this point of view. Understanding the fully biochemical role of its enzymes has determined the scientists to find new separation and identification methods. Objective: Our goal was to develop an optimal HPLC analytical method for separation and identification of Naja haje snake venom components, known for its neurotoxic activity. In addition, we wanted to find out if crude snake venom could inhibit the development of both Gram-positive and Gram-negative bacterial cultures. Materials and Method: Analysis of venom was performed on a HPLC system using a C16 column with UV detection at 210 nm. The analysis was done using two mobile phases, containing different concentrations of acetonitrile and trifluoroacetic acid aqueous solution at different pH values. An elution gradient was set at a flow of 0.60 mL/min. Bactericidal activity was quantified by measuring inhibition diameter around an aseptically disk filled with crude venom using Staphylococcus aureus and Escherichia coli. Results: An optimal HPLC analytical method has been developed by changing different parameters such as the pH value of mobile phase A or the elution gradient. The best resolution were obtained at a pH value of 7.4, in gradient varying from 5% to 45% in mobile phase B. Microbiological studies of the venom showed that Gram-positive bacteria growth was inhibited by crude venom, while on Gram-negative bacteria growth no effect was observed. Inhibition zone is dose-dependent and fresh crude venom is with 30% more potent than venom freeze and kept at -55°C. Conclusions: A comprehensive catalog of venom composition may serve as a starting point for studying structurefunction correlations of individual toxins for the development of new research tools and drugs of potential clinical use.

show abstract

Isolation and characterization of four medium-size disintegrins from the venoms of Central American viperid snakes of the genera Atropoides, Bothrops, Cerrophidion and Crotalus

Cited by 18 publications

References 64 publications

Disintegrins of Crotalus simus tzabcan venom: Isolation, characterization and evaluation of the cytotoxic and anti-adhesion activities of tzabcanin, a new RGD disintegrin

Disintegrins of Crotalus simus tzabcan venom: Isolation, characterization and evaluation of the cytotoxic and anti-adhesion activities of tzabcanin, a new RGD disintegrin

Cell migration inhibition activity of a non-RGD disintegrin from Crotalus durissus collilineatus venom

New RP-HPLC Method for Separation of Naja haje haje Venom and Studies of its Bactericidal Effect

Contact Info

Product

Resources

About