Isolation and characterization of exosomes from blood of patients with mastopathy and breast cancer

Тамкович, С. Н.; Somov, A. K.; Карпухина, К. В.; Grigor’eva, Alina; Yunusova, N. V.; Стахеева, М. Н.; Voytsitskiy, Vladimir E.; Кондакова, И. В.; Laktionov, Pavel P.

doi:10.1063/1.5001654

Cited by 2 publications

(1 citation statement)

References 6 publications

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“…Such a shift can be ascribed to the fact that TEM images dried sEVs, being soft organic and aqueous samples, are likely to be subjected to shrinkage as a consequence of the deposition procedure. Conversely, DLS analysis monitors the sEVs hydrodynamic diameter in solution; moreover the contribution of larger sEVs to the intensity of light scattering is more significant than the smaller ones [32].…”

Section: Resultsmentioning

confidence: 99%

Frizzled-10 Extracellular Vesicles Plasma Concentration Is Associated with Tumoral Progression in Patients with Colorectal and Gastric Cancer

Scavo

Cigliano

Depalo

et al. 2019

Journal of Oncology

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Extracellular vesicles (EVs) are involved in intercellular communication during the carcinogenesis. Our attention has been focused on small EVs (sEVs) protein content in colorectal and gastric cancer (CRC and GC). Frizzled (FZD) proteins, a family of receptors comprised in the Wnt signaling pathway, play an important role in the carcinogenesis of CRC and GC. Here, the expression of a specific FZD protein, namely, FZD-10, was investigated in the sEVs extracted from plasma of patients affected by CRC and GC as involved in canonical and noncanonical Wnt signaling in cancer stem cells with a subsequent modification of cellular heterogeneity, omics reprogramming, and tumor plasticity. The expression of FZD-10 protein in the sEVs extracted from plasma of patients affected by CRC and GC and sEVs from plasma of healthy subjects was evaluated against the level of protein Hsp70, established as EVs specific markers along with CD63 and ALIX proteins. The FZD-10 extract from sEVs isolated from plasma of the controls and the CRC or GC subjects indicated that its expression in oncological patients was higher than in the control group, while, at the end of the treatment, it reached values comparable with the average level of controls. Furthermore, the level of FZD-10 in the whole plasma was found comparable with its level in the sEVs extract. The level of FZD-10 in the sEVs represents a potential reliable biomarker with a valuable prognostic function for the diagnosis of CRC and GC and for monitoring the treatment response.

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Section: Resultsmentioning

confidence: 99%

Frizzled-10 Extracellular Vesicles Plasma Concentration Is Associated with Tumoral Progression in Patients with Colorectal and Gastric Cancer

Scavo

Cigliano

Depalo

et al. 2019

Journal of Oncology

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MALDI-TOF/MS Analysis of Extracellular Vesicles Released by Cancer Cells

Zambonin

2022

Applied Sciences

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The direct shedding of extracellular vesicles (EVs) from the plasma membrane is a recognized fundamental method for the intercellular transfer of properties in both physiological and pathological conditions. EVs are classified according to origin, biogenesis, size, content, surface markers, and/or functional properties, and contain various bioactive molecules depending on the physiological state and the type of the cells of origin including lipids, nucleic acids, and proteins. The presence of tumor-derived EVs in body fluids such as blood, ascites, urine, and saliva, together with the important role played in the tumor microenvironment where they intervene at different levels from oncogenesis to metastasis, make EVs a priority target for cancer studies. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) can play a leading role in the analysis and characterization of EVs and their load due to its intrinsic advantages such as high throughput, low sample consumption, speed, the cost-effectiveness of the analysis, and the ease of use. This work reviews the main MALDI-TOF applications for the analysis and characterization of extracellular vesicles in the tumor field.

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Isolation and characterization of exosomes from blood of patients with mastopathy and breast cancer

Cited by 2 publications

References 6 publications

Frizzled-10 Extracellular Vesicles Plasma Concentration Is Associated with Tumoral Progression in Patients with Colorectal and Gastric Cancer

Frizzled-10 Extracellular Vesicles Plasma Concentration Is Associated with Tumoral Progression in Patients with Colorectal and Gastric Cancer

MALDI-TOF/MS Analysis of Extracellular Vesicles Released by Cancer Cells

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