Isolation and characterization of endophytic bacteria from wild and cultivated soybean varieties

Hung, P. Q.; Kumar, Suresh; Govindsamy, V.; Annapurna, K.

doi:10.1007/s00374-007-0189-7

Cited by 110 publications

(83 citation statements)

References 26 publications

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“…This distribution pattern, in which lower plant parts harbor higher frequencies of endophytes, confirms and extends results reported previously (Das et al 2007). Furthermore, our results indicated that TSA supported more of endophytic bacterial growth than PDA as previously reported by Hung and Annapurna (2004).…”

Section: Resultssupporting

confidence: 89%

Characterization of endophytic bacteria associated with rose plant (Rosa damascena trigintipeta) during flowering stage and their plant growth promoting traits

El-Deeb

Bazaid

Gherbawy

et al. 2012

Journal of Plant Interactions

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Little is known about the bacterial communities associated with the rose plants inhabiting dry desert ecosystems. The aim of this study was to isolate and characterize endophytic bacteria from different organs of rose plant. Endophytic bacteria were observed in healthy roots, stems, leaves, and flowers of rose plant, with a significantly higher density in roots, followed by stems, leaves, and petals. A total of 38 bacterial endophytes were isolated and are closely related phylogenetically to Acetobacter, Acinetobacter, Methylococcus, Bacillus, Micrococcus, Planococcus by 16S rRNA sequence analysis. Six endophytic bacteria were found to produce IAA, solubilize Ca 3 (PO 4 ) 2 and produce siderophore. The six endophytic bacteria all had the capacity to produce hydrolytic enzyme such as cellulase, xylanase, pectinase, amylase, protease, lipase, and chitinase, but difference existed among these isolates.

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Section: Resultssupporting

confidence: 89%

Characterization of endophytic bacteria associated with rose plant (Rosa damascena trigintipeta) during flowering stage and their plant growth promoting traits

El-Deeb

Bazaid

Gherbawy

et al. 2012

Journal of Plant Interactions

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“…While many studies in the past have focussed on plant species of economic importance, including soybean, common bean, cowpea, chickpea and red clover (Delorme et al, 2003;Laranjo et al, 2004;Kuklinsky-Sobral et al, 2005;Duodu et al, 2007;Hung et al, 2007;Laranjo et al, 2008;Ogutcu et al, 2008;Appunu et al, 2009;Chagas et al, 2010;Li et al, 2010;Pule-Meulenberg et al, 2010), native legume species have generally received less attention. In Belgium, legumes are restricted to the Faboideae subfamily and contain 102 plant species in 30 different genera (Lambinon et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

A large diversity of non-rhizobial endophytes found in legume root nodules in Flanders (Belgium)

Meyer

Beuf

Vekeman

et al. 2015

Soil Biology and Biochemistry

117

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Statistical analysis grouped the investigated plant species into six clusters according to the presence of particular NRE. However, no correlations could be found within these six clusters towards plant tribes or ecoregions the plants had been sampled from. Cluster analysis of the ecoregions according to the presence of NRE, revealed correlations between bacterial genera and those areas. However, groups present in the ecoregions did not correlate with the groups present in the different plant clusters. When combining our previous study on rhizobial diversity recovered from the same sampling campaign (De Meyer et al., 2011) with the current study, 84.1% of the isolates belonged to the traditional rhizobia groups and only 15.9% were NRE. The Loamy ecoregion yielded the lowest number of culturable NRE (8.04%) and the Campine ecoregion the highest number (24.19%). The present study highlights the frequent presence of these NRE in root nodules. The occurrence of certain rhizobia was correlated with the presence of particular NRE, suggesting their presence may not be accidental, however their functions remain unclear at this point.

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“…Endophytic microorganisms are important in agriculture and are regarded to be ubiquitous, i.e., they are found in virtually all cultivable plants including soy (Hung et al, 2007;Li et al, 2008), beans (Trujillo-López et al, 2006), corn (Rijavec et al, 2007), cassava (Teixeira et al, 2007), potato (Rasche et al, 2009), rice (Mattos et al, 2008), and peanut (Ibáñez et al, 2009). Cassava (Manihot esculenta Crantz) storage roots have been widely used for human and animal nutrition, mainly in tropical regions.…”

Section: Introductionmentioning

confidence: 99%

Use of PCR-DHPLC with fluorescence detection for the characterization of the bacterial diversity during cassava (Manihot esculenta crantz) fermentation

et al. 2014

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ABSTRACT. Denaturing high-performance liquid chromatography (DHPLC) has been described as a suitable method to study DNA polymorphisms. Here, cassava (Manihot esculenta Crantz) fermentation liquor was examined using DHPLC analysis to characterize the bacterial diversity during the fermentation process. GC-clamped amplicons corresponding to a variable region of the bacterial community 16S rDNA were synthesized using polymerase chain reaction (PCR) and then resolved on a base-composition basis using preparative DHPLC. Eluate fractions were collected at random and used as a source of whole community DNA that could be used to determine the bacterial diversity. As a first approach, GC-clamps were removed from the eluted DNA Bacterial diversity during cassava fermentation using DHPLC fragments using PCR to avoid the possible bias these clamps could cause during the construction of clone libraries. As a second approach, a clone library of each eluate sample was constructed, preserving the GC-clamps of the DNA fragments. The first approach generated 132 bacterial rDNA sequences with an average size of 200 bp, 45% of which had similarity to unculturable or non-classified bacteria. The second approach produced 194 sequences identified as Proteobacteria (48%), uncultured or non-classified environmental bacteria (40%) and Firmicutes (12%). We detected a remarkably greater bacterial diversity using the first approach than the second approach. The DHPLC-PCR method allowed for the fast and non-laborious detection of a vast bacterial diversity that was associated with cassava fermentation, and we conclude that it is a promising alternative for the characterization of the overall microbial diversity in complex samples.

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Isolation and characterization of endophytic bacteria from wild and cultivated soybean varieties

Cited by 110 publications

References 26 publications

Characterization of endophytic bacteria associated with rose plant (Rosa damascena trigintipeta) during flowering stage and their plant growth promoting traits

Characterization of endophytic bacteria associated with rose plant (Rosa damascena trigintipeta) during flowering stage and their plant growth promoting traits

A large diversity of non-rhizobial endophytes found in legume root nodules in Flanders (Belgium)

Use of PCR-DHPLC with fluorescence detection for the characterization of the bacterial diversity during cassava (Manihot esculenta crantz) fermentation

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