Isolation and characterization of eight microsatellite loci from the endangered plant species Hypochaeris salzmanniana (Asteraceae)

Abstract

We report the isolation and characterization of eight microsatellite loci for Hypochaeris salzmanniana, an endangered species endemic to the southwestern coast of Spain and the Atlantic coast of Morocco. A total of 32 alleles were detected across a sample of 45 individuals, with an average number of 4.0 alleles per locus. The average polymorphic information content (PIC) was 0.533 and the observed (H O ) and expected (H E ) heterozygosity values varied from 0.022 to 0.978 and from 0.434 to 0.759, respectively. Five loci exhibited significant deviation from Hardy-Weinberg equilibrium (P B 0.001) and three pairs of loci showed significant linkage disequilibrium (P B 0.01). The eight loci were tested for transferability in three others species (H. arachnoidea, H. glabra, and H. radicata) belonging to the same section of H. salzmanniana. With the exception of locus Hsalz7, all loci successfully amplified in the three species. These preliminary data confirm the usefulness of microsatellite markers for assessing the ecology and genetic structure of H. salzmanniana and to understand the evolution of species within the section Hypochaeris.

Uncertain pollination environment promotes the evolution of a stable mixed reproductive system in the self-incompatible Hypochaeris salzmanniana (Asteraceae)

“…Twenty-five microsatellite loci previously characterized and available for Hypochaeris species (Mix et al, 2004, Ruas et al, 2009L ucio et al, 2011) were tested for amplification following the polymerase chain reaction (PCR) conditions provided with a Veriti TM 96-Well Thermal Cycler (Applied Biosystems, USA). Ten of them produced clear amplicons of the expected size on agarose gels [Hsalz5, Hsalz7, Hsalz12, Hsalz14, Hsalz25, Hsalz30 and Hsalz35 from Ruas et al (2009); and HrGA-9, HrGA-10 and HrGA-12 from Mix et al (2004)].…”

“…Ten of them produced clear amplicons of the expected size on agarose gels [Hsalz5, Hsalz7, Hsalz12, Hsalz14, Hsalz25, Hsalz30 and Hsalz35 from Ruas et al (2009); and HrGA-9, HrGA-10 and HrGA-12 from Mix et al (2004)]. Forward primers were then labelled with fluorescent dyes (VIC, PET, NED and FAM from Applied Biosystems) and PCR products were sequenced on an Applied Biosystems 3730xl DNA Analyzer, using LIZ-500 as internal lane size standard.…”