Isolation and characterization of eight microsatellite loci in <i>Leporinus macrocephalus</i> (Characiformes: Anostomidae) and cross‐species amplification

Morelli, Karina Alessandra; Revaldaves, Eloísa; Oliveira, Cláudio; Foresti, Fausto

doi:10.1111/j.1471-8286.2006.01484.x

Cited by 20 publications

(15 citation statements)

References 2 publications

(1 reference statement)

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“…Many studies have successfully demonstrated heterologous amplification of a target specie with SSR primer (Barbosa et al, 2006;Hatanaka et al, 2006, Morelli et al, 2007, thereby lowering the costs of future projects.…”

Section: Discussionmentioning

confidence: 99%

“…This would be most feasible with cultured species where parents and their progeny can be managed and traced within a closed system. It will be possible to use mapped genetic markers to trace the inheritance of specific chromosomal arms in progeny (Morelli, 2007). The polymorphism obtained with microsatellite markers have provided strong information utilized in the management of fish stocks (Alam and Islam, 2005).…”

Section: Monitoring Genetic Changes In Stocksmentioning

confidence: 99%

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Microsatellite markers in Aquaculture: Application in Fish population genetics

2013

IOSR-JESTFT

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Section: Discussionmentioning

confidence: 99%

Section: Monitoring Genetic Changes In Stocksmentioning

confidence: 99%

Microsatellite markers in Aquaculture: Application in Fish population genetics

2013

IOSR-JESTFT

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“…In the analyses of microsatellite loci of the groups of L. elongatus, eight flanking primer pairs constructed for microsatellite loci of Leporinus macrocephalus (Lmac) (Morelli et al, 2007) were screened. After adjustments of the DNA amplification conditions (concentration of the components of PCR reactions; time and temperature of the different steps in the amplification cycle), as well as the adjustments of electrophoretic analysis methodology, we selected five of these primers pairs for the Lmac 04, 06, 07, 08 and 09 loci.…”

Section: Rapd and Microsatellites Markers Dna Amplificationmentioning

confidence: 99%

Genetic diversity of the species Leporinus elongatus (Teleostei: Characiformes) in the Canoas Complex - Paranapanema River

et al. 2012

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Dams constructed along waterways interrupt the dispersion and migration of aquatic organisms, affecting mainly the abundance of migratory fish species. Translocation mechanisms have been constructed at dams aiming to minimize their impact on fish species migration behavior. There is little information available about the effect of the construction of dams on the genetic structure of the Neotropical migratory fish fauna. Therefore, RAPD molecular markers and microsatellites were utilized to evaluate the diversity and genetic structure of the migratory species Leporinus elongatus (piapara) in the Canoas Complex -Paranapanema River -Brazil. Ten groups were sampled in the fish ladders of the hydroelectric dam Canoas I and Canoas II during the reproductive period in three consecutive years. Both markers showed a high level of genetic diversity within these groups. The microsatellite markers demonstrated a loss of heterozygosity and a considerable level of inbreeding in the species. The genetic differentiation found among the groups with both markers utilized is within a range from low to moderate. The data obtained with the parameter of genetic diversity among the groups led to the conclusion that the groups of L. elongatus of the Canoas Complex are structured as a single population composed of sub-populations with low genetic diversity among them. The data on genetic diversity and population structure of L. elongatus are of great importance for the development of the species management and conservation programs in the Canoas Complex, which can also be utilized in aquaculture programs.As barragens construídas ao longo de sistemas hídricos interrompem a dispersão e a migração dos organismos aquáticos, afetando principalmente a abundância das espécies de peixes migradores. Mecanismos para transposição foram construídos em barragens visando minimizar esses impactos. Poucas são as informações disponíveis sobre o efeito da construção de barragens na estrutura genética populacional da fauna neotropical de peixes migradores. Nesse contexto, marcadores moleculares RAPD e microssatélites foram utilizados para avaliar a diversidade e a estrutura genética da espécie migradora Leporinus elongatus (piapara) no Complexo Canoas -rio Paranapanema -Brasil. Dez grupos foram amostrados nas escadas para transposição de peixes das UHEs Canoas I e Canoas II durante o período reprodutivo em três anos consecutivos. Ambos os marcadores evidenciaram uma alta diversidade genética para esses grupos. Os marcadores microssatélites mostraram uma perda de heterozigosidade e uma considerável taxa de endocruzamento para a espécie. A diferenciação genética encontrada entre os grupos, com ambos os marcadores utilizados, pode ser considerada de moderada a baixa. Os dados obtidos com os parâmetros de diversidade genética entre os grupos permitiram concluir que os grupos de L. elongatus do Complexo Canoas estão estruturados como uma única população composta por sub-populações com baixa diversidade genética entre elas. Os dados obtidos sobre a diversidade ...

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“…Given that the development of primers can be costly, several studies have tested for possible heterologous amplifications in a target species using primers already described for evolutionarily related species (Cipriani et al, 1999;Brondani et al, 2003) or even for evolutionarily related genera (Zhan et al, 2009). Many studies have successfully demonstrated heterologous amplification (Barbosa et al, 2006;Hatanaka et al, 2006, Morelli et al, 2007, thereby lowering the costs of future projects. However, the loci detected by successful heterologous amplification should also be tested for their ability to access the polymorphism in the genome of the target species.…”

Section: Introductionmentioning

confidence: 99%

Short Communication Heterologous amplification and characterization of microsatellite markers in the Neotropical fish Leporinus friderici

Olivatti¹,

Boni²,

Silva-Júnior³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. Leporinus friderici, native to the Amazon Basin and popularly known as "piau-três-pintas", has great ecological and economic importance; it is widely fished and consumed throughout much of tropical South America. Knowledge of the genetic diversity of this native species is important to support management and conservation programs. We evaluated microsatellite loci amplification, using heterologous primers, in 31 individuals of L. friderici. These samples were collected from natural populations of the Araguaia River basin, in central Brazil, and the DNA was extracted from samples of muscle tissue. Eight loci were successfully analyzed. Six of them were polymorphic, and the number of alleles ranged from three to 10. Values of expected heterozygosities for these polymorphic loci ranged from 0.488 to 0.795. Exclusion probability (0.983), the identity probability (0.000073), and the mean genetic diversity values were high, showing that these microsatellite markers are suitable for assessing the genetic variability of L. friderici populations. There is a growing interest in studies that evaluate the genetic variability of natural populations for various purposes, such as conservation. Here, we showed that a viable alternative to the costly development of specific primers for fish populations is simply testing for heterologous amplification of microsatellite markers available from research on other species.

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Isolation and characterization of eight microsatellite loci in Leporinus macrocephalus (Characiformes: Anostomidae) and cross‐species amplification

Cited by 20 publications

References 2 publications

Microsatellite markers in Aquaculture: Application in Fish population genetics

Microsatellite markers in Aquaculture: Application in Fish population genetics

Genetic diversity of the species Leporinus elongatus (Teleostei: Characiformes) in the Canoas Complex - Paranapanema River

Short Communication Heterologous amplification and characterization of microsatellite markers in the Neotropical fish Leporinus friderici

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