Isolation and Characterization of Effective Bacteria That Reduce Ammonia Emission from Livestock Manure

Abstract

Ammonia from livestock manure reacts with chemical components discharged from various emission sources to produce airborne particulate matter. This study aimed to investigate a novel effective microbial agent to suppress ammonia gas emitted from manure. Both isolated L12I and 12III strains, identified as Pediococcus acidilactici (PA), were selected for their superior activity in assays performed with the evaluation criteria such as acid production, ammonia decomposition, and urease inhibition, which are key factors influencing ammonia excretion. The survivability of PA strains was confirmed by an increase in DNA abundance in the manure. PA strains lowered the pH of manure and suppressed the growth of hyper-ammonia-producing bacteria (HAB) possessing urease activity. The L12I and 12III treatment groups showed 23.58% and 38.00% emission reductions, respectively. Especially, the 12III strain was proven to be the more effective strain for reducing ammonia gas emission, with the best ability to reduce pH and inhibit HAB. The strains could have an additive effect in improving the manure quality as a nitrogen fertilizer by preserving the total nitrogen and urea content. These results suggest that PA strains can be used as unprecedented microbial agents to improve manure-derived environmental pollution and improve fertilizer quality.

Microbial agent spraying in pig housing and slurry can potentially reduce harmful gas emissions – a preliminary study

“…Effective microbial products are generally utilised to reduce odour and promote fermentation in agricultural fields, and they include actinomycetes, B. subtilis, lactic acid bacteria, yeasts, etc. (Kim et al, 2022). The latter authors also commended the microbial agent for reducing pH, NH 3 concentration, and urease activity, which are part of emission factors.…”

State-of-the-Art Review on Engineering Uses of Calcium Phosphate Compounds: An Eco-Friendly Approach for Soil Improvement

“…In previous studies [ 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 ], a variety of methods have been used to reduce the ammonium from soil. The most effective method for removing ammonia is the flushing/rinsing method with a reduction rate of 99.8% in aqueous NH 4 + .…”

“…Another approach to solve the ammonium problem is to use different types of bacteria and another source of calcium. By adding the Gram-positive lactic acid bacterium Pediococcus acidilactici , 38% of emission reductions can be achieved [ 58 ]. It has been proven that the use of calcium acetate as a calcium source for MICP could reduce NH 3 emissions by 54.2% [ 59 ].…”

Chinese Herbal Extracts Mitigate Ammonia Generation in the Cecum of Laying Hens: An In Vitro Study

“…Uric acid accounts for about 80% of the total nitrogen in the excreta of laying hens [10]. After uric acid flows back to the cecum, under the action of uricase and urease secreted by microorganisms, it is decomposed to produce NH 4 + and CO 2 [11], and NH 4 + is converted into ammonia under alkaline conditions [12]. Among them, about 60-70% of uric acid in the cecum will be degraded, and about 38% will be converted into ammonia [13].…”