Isolation and Characterization of Dunaliella Species from Sambhar Lake (India) and its Phylogenetic Position in the Genus Dunaliella Using 18S rDNA

Sharma, Pooja; Agarwal, Varsha; Mohan, M. Krishna; Kachhwaha, Sumita; Kothari, S. L.

doi:10.1007/s40009-012-0038-6

Cited by 11 publications

(7 citation statements)

References 23 publications

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“…It is a common observation that Dunaliella cultures taken from their natural habitat (high salinity) respond differently under laboratory growth condition as shown in the present study. Growth rate was reduced under salinity extremes [25] and similar result was observed in D. tertiolecta [1]. High growth rate and high chl-a content have been reported to be related with photosynthesis in the marine alga Fucus vesiculosus [26].…”

Section: Discussionsupporting

confidence: 76%

Expression analysis of photosynthesis genes in Dunaliella salina grown at different NaCl concentrations

2015

J App Biol Biotech

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The expression of five genes involved in photosynthesis was investigated under different salinity conditions in Dunaliella salina. These genes encodes ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) large and small subunits, major light-harvesting chlorophyll (chl) a/b protein of photosystem II, carbonic anhydrase and adenosine tri phosphate synthase alpha subunit. As D. salina grows in hypersaline conditions, it is important to study expression of these genes in relation to different salinity levels. Changes in the mRNA level under different NaCl conditions were determined using the comparative CT method. Cell number and chl a content were estimated to determine optimal growth at different NaCl concentrations. Real-time PCR results showed enhanced expression of photosynthesis genes upon exposure of D. salina cells from Sambhar Lake to 1.7 M NaCl containing medium. This salinity corresponds to only one-third of the salinity of the Sambhar Lake habitat. In another salt condition, the expression level of all genes was found to be down -regulated at 1.0 M (sub-optimal concentration for growth of D. salina), 2.5 M and 3.0 M NaCl (supra-optimal concentration for growth) when compared with 1.7 M NaCl containing medium as a control. The present study will help in understanding the salinity tolerance in D. salina as a model microalga.

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Section: Discussionsupporting

confidence: 76%

Expression analysis of photosynthesis genes in Dunaliella salina grown at different NaCl concentrations

2015

J App Biol Biotech

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“…The variation in carotene production may be explained based on the source. D. salina used in the present study was isolated from salt lake (Shambar lake, Rajasthan, India) (Sharma et al 2012). Carotenoids biosynthesis is associated with Figure 2.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and cytotoxic activity of carotenes produced byDunaliella salinaunder stress

Singh

Baranwal

Reddy

2016

Pharmaceutical Biology

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Context Dunaliella salina Teodoresco (Dunaliellaceae) is one of the promising microalgae consumed as food and medicine for many years. Objective Dunaliella salina was grown under different stress conditions for enhancing carotene production. The carotene enriched extract was evaluated for antioxidant and cytotoxic activity. Materials and methods Carotene content was calculated under salinity, nitrogen and temperature stress conditions. Antioxidant activity was determined through DPPH assay by incubating the samples for 45 min with 250 lg/mL of extract and reducing power assay was performed with 50, 100, 150 and 200 lg/mL of extract. Cytotoxicity was determined by incubating $2 Â 10 4 MCF-7 (breast cancer) cells with 250 lg of extract in each well for 72 h by MTT assay. Result Carotene content was significantly increased to 9.8 (3.5 M NaCl), 13.9 (37 C), 8.2 (250 mM KNO 3 ) and 10.6 lg/mL (nitrogen-depleted medium) as compared with 3.2 lg/mL in normal conditions (1.7 M NaCl, 0.75 mM KNO 3 and 28 C). Free radical scavenging activity increased at 3.0 and 3.5 M NaCl (27.8 and 57.5%, respectively), 37 C (31.4%) and in nitrogen-depleted medium (41.9%) compared with normal (15%) conditions. Carotene content and scavenging activity were positively correlated under salinity (r ¼ 0.97), temperature (r ¼ 0.85) and nitrogen (r ¼ 0.7) stress conditions. Cytotoxicity against MCF-7 cell lines increased due to increase in carotene content suggesting that cytotoxicity may be associated with carotene accumulation. Discussion and conclusions Carotene content enhanced by D. salina under stress conditions increased the antioxidant and cytotoxic activity. ARTICLE HISTORY

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“…Dunaliella salina was isolated (Sharma et al ., 2012) from the Salt River of Shahdad (30°24′16.164′′N 57°40′57.828′′E) in Kerman, Iran and identified based on physiological and morphological descriptions in the references cited in Massyuk (1973c) and Borowitzka & Siva (2007). After transferring the samples to the laboratory, EC (33.5 ds cm −1 ) and pH (7.75) of water were measured.…”

Section: Methodsmentioning

confidence: 99%

Effects of polyploidy on response ofDunaliella salinato salinity

Nezhad

Mansouri

2019

J. Mar. Biol. Ass.

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In this study, polyploidy level was determined by flow cytometry analysis. The effect of polyploidy by colchicine treatment was examined on the growth parameters, malondealdehyde (MDA), as well as activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in response to different levels of salinity in Dunaliella salina. The results of algal growth indicated that 3 M NaCl was the optimal concentration of salt, since the highest enhancement in fresh and dry weight, chlorophyll and carotenoids, soluble sugar, glycerol, protein and starch content was observed in comparison to other concentrations. The amount of these metabolites declined in the concentrations under optimum salinity. The least and highest amounts of MDA were observed at 1 and 4 M NaCl respectively. Polyploidy in optimum concentration of salt, caused further increment of the above growth parameters. In relation to this, in most cases, treatment of 0.1% colchicine was most effective. The beneficial effects of polyploidy in non-optimal conditions were also found in some parameters such as biomass, chlorophyll, carotenoids, proteins and starch. Furthermore, the activity of antioxidant enzymes CAT, SOD and POD showed a positive significant correlation with salt stress and these were maximized at 4 M NaCl. Polyploidy (especially colchicine 0.1%) affected activity of these antioxidant enzymes in some concentrations of salt. Overall, our results suggest that the microalgae has significantly different responses to salt stress based on ploidy levels.

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Isolation and Characterization of Dunaliella Species from Sambhar Lake (India) and its Phylogenetic Position in the Genus Dunaliella Using 18S rDNA

Cited by 11 publications

References 23 publications

Expression analysis of photosynthesis genes in Dunaliella salina grown at different NaCl concentrations

Expression analysis of photosynthesis genes in Dunaliella salina grown at different NaCl concentrations

Antioxidant and cytotoxic activity of carotenes produced byDunaliella salinaunder stress

Effects of polyploidy on response ofDunaliella salinato salinity

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