Isolation and Characterization of DNA from Kinetoplasts of
            <i>Leishmania enriettii</i>

Buy, H. G. du; Mattern, Carl F. T.; Riley, Freddie L.

doi:10.1126/science.147.3659.754

Cited by 56 publications

(11 citation statements)

References 8 publications

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“…The impermewbility s f the kinetoplast membranes to exogenous DNAase is in agreement with studies on Neurosporu mitochondria( 11 ) and on water-lysed ghosts of Leishmania enrielti ( 6).…”

Section: Discussionsupporting

confidence: 87%

“…On the other hand, it has been found that none Olf the species of the Merrillosphaera section has protoplasmic connections in mature coenobia. This is one of the most marked structural differences between Euvolvox and Merrillosphaera (6). However, notwithstanding such a difference, no marked difference in the swimming pattern of the 2 coenolbia has ever been seen.…”

Section: Biological Laboratory Kansai Medical School Hirakata Citymentioning

confidence: 91%

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Behavior of the Kinetoplast of Leishmania tarentolae upon Cell Rupture*

Simpson¹

1968

The Journal of Protozoology

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SYNOPSIS. The kinetoplast of L. tarentolae remains attached to the basal body upon cell Tupture by detergent lysis, sonication, OT hypotonic lysis in 0.02 M Tris buffer (pH 7.9) at 0-4 C. Hypotonic lysis in 0.02 M Tris-HC1-2 mM EDTA at 0-4 C and application of mild shearing forces bring about release of most of the swollen kinetoplasts. The kinetoplast DNA can be seen in phase conttmt microscopy as a dark mass contiguous to the kinetoplast membrane directly opposite the basal body. Upon return to isotonic media, the kinetopkt shrinks; the membranes of such kinetoplasts are impermeable to added DNAase.

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“…The impermewbility s f the kinetoplast membranes to exogenous DNAase is in agreement with studies on Neurosporu mitochondria( 11 ) and on water-lysed ghosts of Leishmania enrielti ( 6).…”

Section: Discussionsupporting

confidence: 87%

Section: Biological Laboratory Kansai Medical School Hirakata Citymentioning

confidence: 91%

Behavior of the Kinetoplast of Leishmania tarentolae upon Cell Rupture*

Simpson¹

1968

The Journal of Protozoology

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“…Electron micrographs (19,11,25,30), electron microscope radio- autographs (1,22,10), and the resistance of mitochondrial DNA to DNase digestion in cell homogenates (7,16,23,29) all indicate that the DNA is within the matrix area of mitochondria. Results obtained with CsC1 density gradient centrifugation indicate that mitochondrial DNA often has a different base ratio than nuclear DNA from the same cell (7,2,16,17,23,26 (17,24,23) and increase in optical density (26,23) when heat-denatured. This indicates that this DNA is double stranded.…”

Section: Discussionmentioning

confidence: 99%

THE DISTRIBUTION OF DNA AMONG DIVIDING MITOCHONDRIA OF TETRAHYMENA PYRIFORMIS

Parsons¹,

Rustad²

1968

The Journal of Cell Biology

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A squash technique was developed for log phase Tetrahymena pyriformis which permitted the resolution of over 100 individual mitochondria from a single cell. Mitochondria incorporated thymidine at all stages of the cell cycle, even when nuclear DNA synthesis was not occurring. During the stage of macronuclear DNA synthesis, however, there was a significant increase in the extent of mitochondrial labeling. Low radioautograph background suggests that mitochondrial DNA is synthesized at the mitochondria themselves. All mitochondria incorporated thymidine-3H within one population-doubling time. Grain counts also showed that the amount of mitochondrial label was retained for four generations and that this label remained randomly distributed among all mitochondria during this time. The results are not consistent with any theory of de-novo or "microbody" origin of mitochondria, but do support the hypothesis that mitochondria are produced by the growth and division of preexisting mitochondria. The stability of the mitochondrial DNA and its distribution among daughter mitochondria satisfy two prerequisites for a genetic material. The possibility is discussed that some of the genetic information for the mitochondrion is contained in the DNA associated with this organelle.

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“…Another problem closely related to the morphologic and physiologic transformation of the brucei-subgroup trypanosomes is the origin of kinetoplastic DNA. As evidenced by its morphologic (70) as well as by its biochemical and biophysical properties ( 13,64,65,69,85), this substance differs in its base composition from nuclear DNA, and it has been suggested (64,65) that its biosynthesis, unlike that of nuclear DNA, involves exogenous rather than endogenously synthesized purines. Altho the nuclear origin of kinetoplastic DNA has been postulated on the basis of 2 isolated electronmicrographs( 9,62) and has been supported by analogous Occurrences in yeast ( 56), there is no categorical evidence for its origin, and the analogy between metabolic changes and mitochondria1 transformations in trypanosomes (see above) and in yeast(for pertinent references see 36) may be more apparenlt than real(96).…”

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confidence: 99%

Some Recent Advances in Our Understanding of Parasitic Protozoa*†

Honigberg

1968

The Journal of Protozoology

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SYNOPSIS. Striking progress in our understanding of parasitic protoroa has been achieved thru the employment of advanced electronmicroscopic, biochemical, immunologic, and cultivation methods. Some recent information gathered by means of these methods on trichomonad and trypanosomalid flagellates as well a s on eimeriidian and plnsmotliid haemosporiidian Sporozoa is discussed. It is empha-T is evident from the recent reports published in a variety I of journals, review articles, and books that the most striking progress in our understanding of parasitic protozoa has been achieved thru the employment of advanced electronmicroscopic, biochemical, immunologic, and cultivation methods. Among the organisms about which much information has been accumulated by {these methods are the trypanosomatid and trichomonad flagellates and the plasmodiid haemosporidians. For this reason, and also because of my relatively greater familiarity with them, the following discussion will deal largely with these assemblages. The elimination of many other groups of parasitic protozoa from the present consideration must not be construed as reflecting lack of activity in their studies, but merely less activity and also poorer knowledge of them on my part.Several recent publications reviewing the parasitic protozoa (21,23,31,36,82,98) indicate (that data pertaining to biochemistry are based to a very significant degree upon studies of organisms grown in culture. Indeed, in many instances, analytic methods could not have been used without large yields of organisms achievable only through cultivation on nonliving media. The observations I propose to make concerning cultivation are relevant almost exclusively to extracellular species. Maintenance of intracellular forms in the presence of host cells or cell cultures, or even free of all such cells(for references see 36), has contributed much to our understanding of leishrnanias, and particularly of malarial parasites. These methods of cultivation involve somewhat different problems, which will not be considered here.

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Isolation and Characterization of DNA from Kinetoplasts of Leishmania enriettii

Abstract: The DNA of Leishmania enriettii can be separated by equilibrium sedimentation in cesium chloride into a major band of density 1.721 and a minor component of density 1.699. DNA from isolated kinetoplasts of this protozoan was identified as the less dense minor component.

Cited by 56 publications

References 8 publications

Behavior of the Kinetoplast of Leishmania tarentolae upon Cell Rupture*

Behavior of the Kinetoplast of Leishmania tarentolae upon Cell Rupture*

THE DISTRIBUTION OF DNA AMONG DIVIDING MITOCHONDRIA OF TETRAHYMENA PYRIFORMIS

Some Recent Advances in Our Understanding of Parasitic Protozoa*†

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