Isolation and Characterization of Diazotrophic Rhizobacteria of Oil Palm Roots

Azlin, C. O.; Amir, H. G.; Chan, Lai Keng

doi:10.21161/mjm.110506

Cited by 2 publications

(2 citation statements)

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“…IAA production by H. seropedicae is one of the mechanisms that could promote growth of the host plant (Bastian et al, 1998). Nevertheless, earlier studies were also revealed that rhizobacteria with the ability to produce phytohormone and fix atmospheric nitrogen can lead to a better growth of oil palm plantlets (Azlin et al, 2005) and rice seedlings (Keyeo et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

Artificial symbiotic association and growth induction of embryogenic calli (Elaeis guineensis Jacq.) inoculated with Herbaspirillum seropedicae (Z78)

Lim, S.-L.,

Nabila, H. M. N.,

Atiqah, S.

et al. 2016

MJM

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Aims:The purpose of this experiment was to determine the artificial symbiosis interaction of Herbaspirillum seropedicae (Z78) on oil palm embryogenic calli. Methodology and results: For this purpose, symbiotic associations were established between Z78 and embryogenic calli of oil palm tissue cultured. A total of five treatments involved, in particular: i) + 3.0 mg/L 2,4-D + 100% N MS medium (control), ii) + Z78 pellet cells (1 mL) + 25% N MS medium, iii) + Z78 supernatant (1 mL) + 25% N MS medium, iv) + Z78 broth culture (1 mL) + 25% N MS medium, and v) + Z78 sonicated cells (1 mL) + 25% N MS medium. All treatments were supplied with minimal N sources (25% N), ammonium nitrate and potassium nitrate, while the control was treated with 100% N sources. Treated samples were harvested on D80 and observed for biomass and diameter increment (%), formation of embryoids, and Z78 colonization. The results showed embryogenic calli in the inoculated treatments that contained depleted N produced similar result to the control treatment which contained 100% N nutrients. Positive interactions occurred between the diazotroph and host plant tissues as viewed under FESEM and EFTEM. Among the treatments, Z78 sonicated cell showed better growth of embryogenic calli compared to others. Conclusion, significance and impact study:The in vitro nitrogen-depleted artificial symbiosis environment allowed the diazotroph (Z78) to be expressed and provide the nitrogen sources and indole-3-acetic acid for cell growth. This study represents beneficial co-culture interaction effects of different inocula of diazotrophic bacterial cells with in vitro embryogenic calli of oil palm.

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Section: Resultsmentioning

confidence: 99%

Artificial symbiotic association and growth induction of embryogenic calli (Elaeis guineensis Jacq.) inoculated with Herbaspirillum seropedicae (Z78)

Lim, S.-L.,

Nabila, H. M. N.,

Atiqah, S.

et al. 2016

MJM

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“…There are growing number of isolates, collected from various host plants. PGPR can be isolated from agricultural crops such as wheat (Majeed et al, 2015), rice (Tan et al, 2014), and oil palm (Azlin et al, 2005). Various successful application of PGPR on agricultural crops such as wheat (Kumar et al, 2017), maize (Sood et al, 2017), rice (Singh et al, 2016), bell pepper (Tariq et al, 2014), and oil palm (Zakry et al, 2012) were reported.…”

Section: Introductionmentioning

confidence: 99%

Selecting the most effective plant growth-promoting bacteria from oil palm (Elaeis guineensis Jacq) roots

Zakry

Ammal

Malahubban

et al. 2019

J Bangladesh Agric Univ

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A total of 30 bacterial isolates were isolated from soil, rhizoplane, and internal tissue of oil palm roots. The isolates were qualitatively tested for their potential to fix N2, solubilize inorganic P and K, and produce phytohormone indole-3-acetic acid. Of the 30 isolates, six isolates were able to exhibit multiple beneficial traits. All six isolates were then identified based on fatty acid methyl esters profile as Escherichia coli strain EX2, Serratia sp. strain EN1, Pantoea ananatis strain EN3, Bacillus sp. strain EN5, Pantoea ananatis strain EN8 and Pantoea sp. strain EN9. Subsequently, all shortlisted isolates were evaluated for plant growth-promoting potential by using shallot as a test plant. The plant test showed no significant difference (p>0.05) between inoculated and uninoculated plants except for Pantoea sp. strain EN9 inoculation which increased significantly (p<0.05) total root length over uninoculated control. Host specificity and IAA capacity of the isolates may be among the important factors affecting their effectiveness in plant growth promotion. J Bangladesh Agril Univ 17(3): 344–348, 2019

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Isolation and Characterization of Diazotrophic Rhizobacteria of Oil Palm Roots

Cited by 2 publications

References 0 publications

Artificial symbiotic association and growth induction of embryogenic calli (Elaeis guineensis Jacq.) inoculated with Herbaspirillum seropedicae (Z78)

Artificial symbiotic association and growth induction of embryogenic calli (Elaeis guineensis Jacq.) inoculated with Herbaspirillum seropedicae (Z78)

Selecting the most effective plant growth-promoting bacteria from oil palm (Elaeis guineensis Jacq) roots

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