Isolation and Characterization of Desulfitobacterium sp. strain Y51 Capable of Efficient Dehalogenation of Tetrachloroethene and Polychloroethanes

Suyama, Akira; Iwakiri, Ryo; Kai, Keiichirou; Tokunaga, Takashi; Sera, Nobuyuki; Furukawa, Koichi

doi:10.1271/bbb.65.1474

Cited by 109 publications

(102 citation statements)

References 43 publications

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“…Chloroethenes were quantified using a GC-8A PF GC (Shimadzu, Kyoto, Japan) equipped with a flame ionization detector (FID) and a silicon DC-550 column, as described previously. 24) Ethene was quantified using a GC-14B GC (Shimadzu) equipped with an FID and a Sunpak A column (Shinwa Chemical Industries, Kyoto, Japan). The injector and detector temperatures were set at 150 C, and the column temperature at 60 C. Methane was quantified using a GC-14B GC (Shimadzu) with a thermal conductivity detector and a ShinCarbon ST column (Shimadzu).…”

Section: Methodsmentioning

confidence: 99%

Enrichment and Characterization of a Trichloroethene-Dechlorinating Consortium Containing Multiple “Dehalococcoides” Strains

Futagami¹,

Fuyuki²,

Hashimoto³

et al. 2011

Bioscience, Biotechnology, and Biochemistry

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Section: Methodsmentioning

confidence: 99%

Enrichment and Characterization of a Trichloroethene-Dechlorinating Consortium Containing Multiple “Dehalococcoides” Strains

Futagami¹,

Fuyuki²,

Hashimoto³

et al. 2011

Bioscience, Biotechnology, and Biochemistry

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“…At the same time, Desulfitobacterium hafniense was described with the type strain DCB-2 T (=DSM 10664 T ) 30) , which is able to dechlorinate pentachlorophenol and 3-chloro-4-hydroxyphenylacetate but not PCE. Some other strains of Desulfitobacterium hafniense were reported to dechlorinate pentachlorophenol 22,79,122) , 2,4,6-trichlorophenol 24) , 3-chloro-4-hydroxy-phenylacetate 51,52) , and PCE 51,120) . Strain PCP-1 was isolated from a methanogenic consortium and classified originally as a new species of the genus Desulfitobacterium with the name Desulfitobacterium frappieri 22) .…”

Section: Desulfitobacterium Chlororespirans Strain Co23mentioning

confidence: 99%

Biodiversity of Dehalorespiring Bacteria with Special Emphasis on Polychlorinated Biphenyl/Dioxin Dechlorinators

Hiraishi

2008

Microb. Environ.

103

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A wide variety of haloorganic compounds undergo reductive dehalogenation by certain anaerobic microorganisms. Metabolic reductive dehalogenation is coupled with energy-conserving respiratory electron transport in which a halogenated compound is used as the terminal electron acceptor, the biological process called dehalorespiration or halorespiration. Dehalorespiring bacteria may play important roles in the geochemical cycle with organohalogens in nature and have great promise in their application to the bioremediation of haloorganic contaminants derived from anthropogenic sources. During the past decade, a number of dehalorespiring microorganisms, including a unique group of strictly dehalorespiring bacteria, "Dehalococcoides", have been isolated and characterized at phylogenetic, physiologic, and genetic levels. Also, new perspectives of dehalorespiring bacteria have emerged based on information about genomics and molecular microbial ecology. This review article focuses on up-to-date knowledge of the biodiversity of dehalorespiring bacteria and reductively dehalogenating microbial consortia with special emphasis on those capable of transforming polychlorinated biphenyls and dioxins.

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“…Although several strains can use PCE or trichloroethene (TCE) as an electron acceptor, no Desulfitobacterium strain isolated so far completely dechlorinates these compounds to ethene (7,14,48). In contrast to Dehalococcoides strains, Desulfitobacterium strains can utilize electron acceptors other than chlorinated compounds.…”

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Complete Genome Sequence of the Dehalorespiring Bacterium Desulfitobacterium hafniense Y51 and Comparison with Dehalococcoides ethenogenes 195

et al. 2006

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Desulfitobacterium strains have the ability to dechlorinate halogenated compounds under anaerobic conditions by dehalorespiration. The complete genome of the tetrachloroethene (PCE)-dechlorinating strain Desulfitobacterium hafniense Y51 is a 5,727,534-bp circular chromosome harboring 5,060 predicted protein coding sequences. This genome contains only two reductive dehalogenase genes, a lower number than reported in most other dehalorespiring strains. More than 50 members of the dimethyl sulfoxide reductase superfamily and 30 paralogs of the flavoprotein subunit of the fumarate reductase are encoded as well. A remarkable feature of the genome is the large number of O-demethylase paralogs, which allow utilization of lignin-derived phenyl methyl ethers as electron donors. The large genome reveals a more versatile microorganism that can utilize a larger set of specialized electron donors and acceptors than previously thought. This is in sharp contrast to the PCE-dechlorinating strain Dehalococcoides ethenogenes 195, which has a relatively small genome with a narrow metabolic repertoire. A genomic comparison of these two very different strains allowed us to narrow down the potential candidates implicated in the dechlorination process. Our results provide further impetus to the use of desulfitobacteria as tools for bioremediation.Halogenated organic compounds are released into the environment from natural and anthropogenic sources. Many anthropogenic halogenated chemicals, like chlorinated haloalkenes (7, 10, 46), benzenes (1), and dioxins (5), are of particular concern due to their toxicity to humans and other forms of life. This toxicity is often paired with high recalcitrance to degradation, especially in anaerobic environments, leading to persistent contamination.Anaerobic environments are frequently characterized by limited availability of electron acceptors. Theoretical calculations have shown that coupling the reduction of many halogenated organic compounds to the oxidation of suitable substrates is a way to harness energy (46). As determined two decades ago, this source of energy is utilized by the microbial community. The oxidation of available electron donors coupled to the reduction of halogenated organic compounds while energy is conserved is called dehalorespiration (7,10,46). Dehalorespiring strains have been isolated independently from contaminated sites around the world. The two most prominent genera resulting from these isolation efforts are Dehalococcoides (29) and Desulfitobacterium (51), and various strains of these genera are used as model systems to study dehalorespiration (8,11,51).Dehalococcoides ethenogenes 195 is one of the few strains isolated to date which can dechlorinate tetrachloroethene (PCE) to ethene (29). D. ethenogenes 195 can use only hydrogen as an electron donor and chlorinated compounds as electron acceptors (29).Desulfitobacterium strains are also known to dechlorinate a wide variety of substrates, including halophenolic compounds and chloroalkenes (7,10,46). Although several s...

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Isolation and Characterization of Desulfitobacterium sp. strain Y51 Capable of Efficient Dehalogenation of Tetrachloroethene and Polychloroethanes

Cited by 109 publications

References 43 publications

Enrichment and Characterization of a Trichloroethene-Dechlorinating Consortium Containing Multiple “Dehalococcoides” Strains

Enrichment and Characterization of a Trichloroethene-Dechlorinating Consortium Containing Multiple “Dehalococcoides” Strains

Biodiversity of Dehalorespiring Bacteria with Special Emphasis on Polychlorinated Biphenyl/Dioxin Dechlorinators

Complete Genome Sequence of the Dehalorespiring Bacterium Desulfitobacterium hafniense Y51 and Comparison with Dehalococcoides ethenogenes 195

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