Isolation and characterization of dermatan sulphate proteoglycan from human uterine cervix

Uldbjerg, Niels; Malmström, Anders; Ekman, Gunvor; Sheehan, John K.; Ulmsten, Ulf; Wingerup, L.

doi:10.1042/bj2090497

Cited by 92 publications

(53 citation statements)

References 21 publications

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“…fibronectins [55,56]. The proteoglycans found in the cervix include decorin, fibromodulin, biglycan, versican, aggrecan and heparan sulfate proteoglycan [35,38,57,58]. Examination of cervical biopsies from nonpregnant women with a history of cervical insufficiency suggests that increased distensibility of the cervix during pregnancy can be a result of prepregnancy decreased collagen concentration and perhaps a prepregnancy increased smooth muscle content, i.e.…”

Section: Importance Of the Studymentioning

confidence: 99%

The transcriptome of cervical ripening in human pregnancy before the onset of labor at term: Identification of novel molecular functions involved in this process

Hassan¹,

Romero²,

Tarca³

et al. 2009

The Journal of Maternal-Fetal & Neonatal Medicine

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Objective. The aim of this study was to identify changes in the cervical transcriptome in the human uterine cervix as a function of ripening before the onset of labor. Study Design. Human cervical tissue was obtained from women at term not in labor with ripe (n ¼ 11) and unripe (n ¼ 11) cervices and profiled using Affymetrix GeneChip 1 HGU133Plus2.0 arrays. Gene expression was analyzed using a moderated t-test (False Discovery Rate 5%). Gene ontology and pathway analysis were performed. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used for confirmation of selected differentially expressed genes. Results.(1) Ninety-one genes were differentially expressed between ripe and unripe groups. (2) Cervical ripening was associated with enrichment of specific biological processes (e.g. cell adhesion, regulation of anatomical structure), pathways and 11 molecular functions (e.g. extracelluar matrix (ECM)-structural constituent, protein binding, glycosaminoglycan binding). (3) qRT-PCR confirmed that 9 of 11 tested differentially expressed genes (determined by microarray) were upregulated in a ripe cervix (e.g. MYOCD, VCAN, THBS1, COL5A1). (4) Twenty-three additional genes related to ECM metabolism and adhesion molecules were differentially regulated (by qRT-PCR) in ripe cervices. Conclusion.(1) This is the first description of the changes in the human cervical transcriptome with ripening before the onset of labor. (2) Biological processes, pathways and molecular functions were identified with the use of this unbiased approach. (3) In contrast to cervical dilation after term labor, inflammation-related genes did not emerge as differentially regulated with cervical ripening. (4) Myocardin was identified as a novel gene upregulated in human cervical ripening.

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Section: Importance Of the Studymentioning

confidence: 99%

The transcriptome of cervical ripening in human pregnancy before the onset of labor at term: Identification of novel molecular functions involved in this process

Hassan¹,

Romero²,

Tarca³

et al. 2009

The Journal of Maternal-Fetal & Neonatal Medicine

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“…In contrast, PG isolated from human uterine cervix contained only dermatan sulfate as GAG (Uldbjerg et al 1983). In the present study, SPG in myometrium were labeled in vitro with [3551 sulfate and extracted with 4 M guanidine hydrochloride.…”

Section: Discussionmentioning

confidence: 99%

“…Although PG in many tissues have been studied, little is known about uterine PG as stated by Anderson (1982). Of the uterine PG, dermatan sultate PG was recently isolated from human uterine cervix (Uldbjerg et al 1983).…”

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Sulfated proteoglycans synthesized in myometrium of the estrogen-treated rabbit.

Munakata

Isemura

Yosizawa

1984

Tohoku J. Exp. Med.

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ZENSAKUSchool of MUNAKATA, II., ISEMURA, M. and YoSIzAwA, Z. Sulfated Proteoglycans Synthesized in Myometrium of the Estrogen-Treated Rabbit. Tohoku J. exp. Med., 1984, 144 (3), 291-297 Myometrium obtained from the estrogen-treated rabbit was incubated with [35S] sulfate, and the 35S-labeled proteoglycans were extracted from this tissue with 4 M guanidine hydrochloride. The extract was fractionated by CsCI density gradient centrifugation, followed by DEAE-cellulose column chromatography. The major proteoglycan fraction (D(1+2)) was separated into two fractions (D(1+2)-I and D(1+2)-II) by gel filtration on Sepharose CL-4B. The compositions of the 35S-labeled glycosaminoglycans in 5 proteoglycan fractions (D(1+2)-I, D(1+2)-II, D3, D4, and D5) were analyzed by two dimensional cellulose acetate membrane electrophoresis after pronase digestion. All the fractions contained the 35S-labeled chondroitin sulfates A/C and heparan sulfate with the different proportions of the radioactivities. The results of the present study indicated that sulfated proteoglycans newly synthesized in myometrium of the estrogen-treated rabbit were considerably heterogeneous not only in the 35S-labeled glycosaminoglycan compositions but also in the molecular weight and buoyant density. sulfated proteoglycan ; proteoglycan ; myometrium ; estrogen ; rabbit myometriumIn a previous paper (Endo et al. 1978), the composition of acidic glycoconjugates in myometrium of the estrogen-treated rabbit was studied. The results indicated that the myometrium was abundant in chondroitin sulfate isomers (chondroitin sulfates A plus C plus dermatan sulfate), amounting to be more than 60% of the total acidic glycoconjugates. In addition, considerable amounts of heparan sulfate and hyaluronic acid were also present. Recently, we found that uterus of the estrogen-treated rabbit actively synthesized chondroitin sulfates A/ C and heparan sulfate (Munakata et al. 1984). These investigations were, however, performed by means of proteolysis to solubilize glycosaminoglycans (GAG)

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“…During pregnancy, the placenta (34) and uterus (35) are rich in DS proteoglycan. The mechanism by which this DS proteoglycan might appear in blood is unknown.…”

Section: Discussionmentioning

confidence: 99%

The Pregnant Woman and Her Fetus

Ford

2002

The Prenatal Person

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Isolation and characterization of dermatan sulphate proteoglycan from human uterine cervix

Cited by 92 publications

References 21 publications

The transcriptome of cervical ripening in human pregnancy before the onset of labor at term: Identification of novel molecular functions involved in this process

The transcriptome of cervical ripening in human pregnancy before the onset of labor at term: Identification of novel molecular functions involved in this process

Sulfated proteoglycans synthesized in myometrium of the estrogen-treated rabbit.

The Pregnant Woman and Her Fetus

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