Isolation and Characterization of Dental Pulp Stem Cells from a Patient with Papillon–Lefèvre Syndrome

Taşlı, Pakize Neslihan; Tapşın, Sıdıka; Demirel, Sezin; Yalvaç, Mehmet Emir; Akyüz, Serap; Yarat, Ayşen; Şahın, Fikrettin

doi:10.1016/j.joen.2012.09.024

Cited by 14 publications

(7 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The hTGSCs, hDPSCs, and hPLSCs were isolated and characterized as described previously; [16][17][18] The hTGSCs were collected from the mandibular third molar tooth, and the hDPSCs and hPDLSCs were…”

Section: Isolation Of Htgscs Hdpscs and Hpdlscs And Cell Culture Comentioning

confidence: 99%

“…Isolated hTGSCs, hDPSCs, and hPDLSCs (passage 3) were characterized for their mesenchymal cell surface profiles, as described previously. [16][17][18] The hTGSCs and hDPSCs were trypsinized and incubated with the following conjugated antibodies: CD29, CD34, CD45, CD73, CD90, CD105, CD133, and CD166 (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA). The hPDLSCs were then incubated with primary antibodies raised against STRO-1, CD146, CD90, CD44, CD19, or CD14.…”

Section: Flow Cytometry-based Mesenchymal Stem Cell Characterizationmentioning

confidence: 99%

See 1 more Smart Citation

Effect of a novel bioceramic root canal sealer on the angiogenesis-enhancing potential of assorted human odontogenic stem cells compared with principal tricalcium silicate-based cements

et al. 2020

Self Cite

View full text Add to dashboard Cite

Objective: This study evaluated the angiogenesis-enhancing potential of a tricalcium silicate-based mineral trioxide aggregate (ProRoot MTA), Biodentine, and a novel bioceramic root canal sealer (Well-Root ST) in human dental pulp stem cells (hDPSCs), human periodontal ligament stem cells (hPLSCs), and human tooth germ stem cells (hTGSCs). Methodology: Dulbecco's modified Eagle's medium was conditioned for 24 h by exposure to ProRoot MTA, Biodentine, or Well-Root ST specimens (prepared according to the manufacturers' instructions). The cells were cultured in these conditioned media and their viability was assessed with 3-(4,5-dimethyl-thiazol-2-yl)-5-(3-carboxy-methoxy-phenyl)-2-(4-sulfo-phenyl)-2H tetrazolium (MTS) on days 1, 3, 7, 10, and 14. Angiogenic growth factors [platelet-derived growth factor (PDGF), basic fibroblast growth factor (FGF-2), and vascular endothelial growth factor (VEGF)] were assayed by sandwich enzyme-linked immunosorbent assay (ELISA) on days 1, 7, and 14. Human umbilical vein endothelial cell (HUVEC) migration assays were used to evaluate the vascular effects of the tested materials at 6-8 h. Statistical analyses included Kruskal-Wallis, Mann-Whitney U, and Friedman and Wilcoxon signed rank tests. Results: None of tricalcium silicate-based materials were cytotoxic and all induced a similar release of angiogenic growth factors (PDGF, FGF-2, and VEGF) (p>0.05). The best cell viability was observed for hDPSCs (p<0.05) with all tricalcium silicate-based materials at day 14. Tube formation by HUVECs showed a significant increase with all tested materials (p<0.05). Conclusion: The tricalcium silicate-based materials showed potential for angiogenic stimulation of all stem cell types and significantly enhanced tube formation by HUVECs.

show abstract

Section: Isolation Of Htgscs Hdpscs and Hpdlscs And Cell Culture Comentioning

confidence: 99%

Section: Flow Cytometry-based Mesenchymal Stem Cell Characterizationmentioning

confidence: 99%

Effect of a novel bioceramic root canal sealer on the angiogenesis-enhancing potential of assorted human odontogenic stem cells compared with principal tricalcium silicate-based cements

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…[42][43][44] Adipogenic differentiation was previously investigated to characterize the stem cells or evaluate some materials. 18,25,30 However, until this moment, it was not used to quantify the lipid vesicle deposition induced by PBM. Similarly, in osteogenic differentiation and ALP activity, the adipogenic differentiation was increased in our study by PBM from 7 to 14 days (p < 0.05).…”

Section: Discussionmentioning

confidence: 99%

“…(a) Osteogenic differentiation: αMEM supplemented with 1% penicillin and streptomycin, 10% FBS, 100 nM dexamethasone (Sigma-Aldrich, USA), 0.05 μM ascorbate-2-phosphate (Sigma-Aldrich, USA) and 10 mM β-glycerophosphate (Sigma-Aldrich); 24 (b) Adipogenic differentiation: αMEM supplemented with 1% penicillin, 10% FBS, 1 mmol/L dexamethasone, 5 μg∕mL −1 bovine insulin (Sigma-Aldrich), 0.5 mmol/L isobutylmethylxanthine (Sigma-Aldrich), and 60 mmol/L indomethacin (Sigma-Aldrich); 25 and (c) Chondrogenic differentiation: αMEM supplemented with 1% penicillin and streptomycin, 10% FBS, 50 nmol/L ascorbic acid 2-phosphate, 6.25 mg/mL bovine insulin, and 10 ng/mL transforming growth factor-beta 1 (TGF-β1/ Sigma-Aldrich). 26…”

Section: Stem Cell Characterizationmentioning

confidence: 99%

Photobiomodulation therapy improves multilineage differentiation of dental pulp stem cells in three-dimensional culture model

et al. 2018

View full text Add to dashboard Cite

Photobiomodulation therapy (PBM) has shown positive effects on stem cell differentiation in monolayer cell culture model, but little is known about its effect on three-dimensional (3-D) agarose gel culture. This study evaluated the PBM effect of human dental pulp stem cells (hDPSCs) differentiation and phosphatase alkaline activity (ALP) using an agarose 3-D model under different nutritional conditions. hDPSCs were characterized and seeded on a 0.3% agarose gel layer with different media (osteogenic, adipogenic, chondrogenic) and were assigned into four groups: control 10% fetal bovine serum (FBS), control 5% FBS, PBM 10% FBS, and PBM 5% FBS. Irradiation was performed with continuous-wave InGaAlP laser, 660 nm, 100 mW, 3,3 J / cm2, spot size 0.3 cm2, 10 s of exposure time, and 1 J of energy per point with 6-h interval between sessions. All groups were evaluated at 7 and 14 days. ALP assay was performed to analyze the deposition of mineralized tissue. At 7 days, PBM 5% FBS group presented better stimulation in osteogenic and adipogenic differentiation compared with control. After 14 days, hDPSCs cultured in 3-D exhibited osteogenic, adipogenic, and chondrogenic differentiation; furthermore, compared to control, PBM significantly stimulated all differentiation processes (p < 0.05). It can be concluded that hDPSCs cultured in 3-D agarose associated to PBM could be a promising tool for tissue engineering applications.

show abstract

“…The odontogenic differentiation was conducted by treating the cells with differentiation medium containing DMEM, 10% (v/v) FBS, 10 -8 M dexamethasone, 5 mmol/L KH 2 PO 4 , and 50 µg/mL ascorbic acid, with or without melatonin (Taşlı et al, 2013a). Eventually, myogenic differentiation was done with DMEM medium supplemented with 5% (v/v) horse serum, 0.1 µM dexamethasone, and 50 µM hydrocortisone (Sigma) (Taşlı et al, 2013b). The myogenic differentiation took 3 weeks with medium changed every 2-3 days.…”

Section: Differentiation Processmentioning

confidence: 99%

Role of melatonin on differentiation of mesenchymal stem cellsderived from third molar germ tissue

Aydoğdu¹,

Taşlı²,

Şişli³

et al. 2016

Turk J Biol

Self Cite

View full text Add to dashboard Cite

Stem cell-based applications have become a popular and promising approach for therapy for a number of disorders including neurodegenerative diseases, degenerative muscle diseases, and osteoporosis, as well as trauma, inflammations, burns, and injuries. Human tooth germ stem cells are an adult stem cell source; they have mesenchymal stem cell properties and show high proliferative and differentiation capacity. Melatonin has been demonstrated to regulate differentiation of human and mouse mesenchymal stem cells into various cell lineages in addition to its other functions in the body. In the current study, the effects of melatonin on osteogenic, neurogenic, adipogenic, chondrogenic, myogenic, and odontogenic differentiation of human tooth germ stem cells were investigated. The results showed that melatonin increases the viability of cells. It significantly augments osteogenic, neurogenic, chondrogenic, myogenic, and odontogenic differentiation of the cells, whereas it reduces adipogenic differentiation capability. These results suggest that melatonin has a great potential to increase differentiation capacity of human tooth germ stem cells and might be useful in regenerative therapy applications involving stem cell differentiations in addition to defining potential treatments for obesity because of its suppressor effects on adipogenesis.

show abstract

Isolation and Characterization of Dental Pulp Stem Cells from a Patient with Papillon–Lefèvre Syndrome

Cited by 14 publications

References 36 publications

Effect of a novel bioceramic root canal sealer on the angiogenesis-enhancing potential of assorted human odontogenic stem cells compared with principal tricalcium silicate-based cements

Effect of a novel bioceramic root canal sealer on the angiogenesis-enhancing potential of assorted human odontogenic stem cells compared with principal tricalcium silicate-based cements

Photobiomodulation therapy improves multilineage differentiation of dental pulp stem cells in three-dimensional culture model

Role of melatonin on differentiation of mesenchymal stem cellsderived from third molar germ tissue

Contact Info

Product

Resources

About