Isolation and characterization of deleterious Pseudomonas aeruginosa KC1 from rhizospheric soils and its interaction with weed seedlings

Lakshmi, Vijaya; Kumari, Sushma; Singh, Ajit; Prabha, Chander

doi:10.1016/j.jksus.2014.04.007

Cited by 37 publications

(15 citation statements)

References 32 publications

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“…Deleterious microorganisms inhibit plant growth via several mechanisms [ 6 , 7 ], which include the production of phytotoxins such as cyanide as well as other volatile and nonvolatile compounds [ 8 , 9 ]. However, plant growth inhibition through phytohormone production is also thought to be an alternative mechanism of this group of microbes.…”

Section: Introductionmentioning

confidence: 99%

Effect of Ammonia and Indole-3-acetic Acid Producing Endophytic Klebsiella pneumoniae YNA12 as a Bio-Herbicide for Weed Inhibition: Special Reference with Evening Primroses

Kang

Bilal

Shahzad

et al. 2020

Plants

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Information on the use of endophytic bacteria as a bio-herbicide for the management of weed control in agricultural fields is limited. The current study aimed to isolate endophytic bacteria from evening primroses and to screen them for their bio-herbicidal activity. Two isolated endophytic bacteria (Pantoea dispersa YNA11 and Klebsiella pneumoniae YNA12) were initially screened for citrate utilization and for indole-3-acetic acid (IAA) and catalase production. The preliminary biochemical assessment showed YNA12 as a positive strain. Ammonia, catalase, and IAA in its culture filtrate were quantified. Gas Chromatography/Mass Spectroscopy- Selective Ion Monitoring (GC/MS-SIM) analysis revealed the production of IAA by YNA12 in a time-dependent manner. YNA12 also exhibited significant ammonia-producing potential and catalase activity against hydrogen peroxide. The YNA12 culture filtrate significantly inhibited the germination rate of evening primrose seeds, resulting in a marked reduction in seedling length and biomass compared with those of the control seeds. Moreover, the culture filtrate of YNA12 significantly accelerated the endogenous abscisic acid (ABA) production and catalase activity of evening primrose seedlings. Macronutrient regulation was adversely affected in the seedlings exposed to the culture filtrate of YNA12, leading to inhibition of seed germination. The current results suggest that endophytic YNA12 may be used as a potent bio-herbicidal agent for controlling weed growth and development.

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Section: Introductionmentioning

confidence: 99%

Effect of Ammonia and Indole-3-acetic Acid Producing Endophytic Klebsiella pneumoniae YNA12 as a Bio-Herbicide for Weed Inhibition: Special Reference with Evening Primroses

Kang

Bilal

Shahzad

et al. 2020

Plants

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“…Meanwhile, C. arvensis was slightly affected by most treatments except for P. syringae st.1 which reduced seedling biomass by 22.83% at 120μg ml -1 (Omer & Balah, 2011). Lakshmi et al (2015) used Pseudomonas aeruginosa KC1 in a form of bacterial suspension to inhibit seedling growth of Portulaca oleracea both in vitro and in glasshouse studies where it caused a high reduction in root length and biomass. To a large extent, this is in line with results of our study; treatment with culture filtrate of Pseudomonas aeruginosa resulted in 100% reduction on seed germination, shoot and root length of Portulaca oleracea while, using the crude ethyl acetate extracts resulted in 71.27% significant reduction in total biomass fresh weight of seedlings at 40mg/ml.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Bacteria from Soil and Rhizosphere as Herbicidal Candidates of Some Broadleaf Weeds

et al. 2019

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T HE USE of bacteria in biological weed control is an alternative ecofriendly way to tackle weed problems and reduce the risk of herbicide resistance. In this study, we obtained four main active bacterial isolates under the genera Pseudomonas, Bacillus and Xanthomonas from Wadi El Natroun region. All the tested bacterial isolates caused high significant reductions in seed germination and seedling growth of Convolvulus arvensis and Portulaca oleracea. Bioassaying ethyl acetate crude extracts of these isolates showed that Pseudomonas sp. (isolate 1) was the most active against seedling stage of Portulaca oleracea. The cultural filtrate of the same isolate caused 100% reduction when assayed on seed germination, shoot and root length of Convolvulus arvensis and Portulaca oleracea. At the highest concentration (40mg/ml) of the crude ethyl acetate extract, the reduction percentage in total biomass fresh weights of Portulaca oleracea and Convolvulus arvensis seedlings reached 71.27 and 39.37%, respectively. The EC 50 values (concentration that inhibited growth by 50%) were 1.3 and 1.64mg/ml for Portulaca oleracea and Convolvulus arvensis, respectively. Comparative 16S rRNA gene sequencing analysis and biochemical characterization demonstrated that isolate FS15 was member of the genus Pseudomonas and belongs to the Pseudomonas aeruginosa group with Pseudomonas aeruginosa strain NR 113599.1 as the closest relative (99.5% sequence similarity). In conclusion, Pseudomonas aeruginosa has high potential to be developed as natural bacterial herbicide and may be used in broadleaf weed control.

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“…Our findings are similar to those of [26], who reported maximum production of HCN, where color intensity ranged from yellow to brown in five bacterial isolates of apple and pear. Mechanisms controlling plant pathogens through HCN production by rhizobacteria includes, induction of plant resistance, blocking of cytochrome oxidase activity, increase in nutrient availability [18], [28], [29].…”

Section: ) Hcn Productionmentioning

confidence: 99%

Optimization of Indole-3-Acetic Acid Production by Diazotrophic B. subtilis DR2 (KP455653), Isolated from Rhizosphere of Eragrostis cynosuroides

Kumari¹,

Prabha²,

Singh³

et al. 2018

IJPMBS

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Diazotrophic rhizobacteria, trigger and enhance plant growth as well as yield through various mechanisms, so their use can reduce the application frequency of chemical fertilizers. Indole-3-acetic acid (IAA), a most common natural auxin influences several physiological processes of the plant's health. The present study is aimed to optimize the conditions for IAA production, along with assay for plant growth promoting traits of Bacillus subtilis DR2 (KP455653), which is a diazotrophic Gram positive, rod bacterium, isolated from rhizosphere of road side weed, Eragrostis cynosuroides from Danapur, Patna, Bihar, India. The screening for IAA production was done in JNFbˉ broth with tryptophan (1 g.l-1) and without tryptophan at pH 5.8, 30±2 °C temperature and 48 h incubation. 137.81 µg.ml-1 and 100.26 µg.ml-1 IAA was produced in Trp + and Trpmedia, respectively. Under various optimized conditions, maximum IAA was produced at 96 h incubation (137.81 µg.ml-1), 35 °C temperature (141.92 µg.ml-1), pH 7 (158.79 µg.ml-1), mannitol as carbon (160.85 µg.ml-1) and ammonium sulfate as nitrogen (162.93 µg.ml-1) sources with tryptophan at final concentration of 1.2 µg.ml-1 (168.09 µg.ml-1), which enhanced the production by 1.2 fold. The findings suggest that B. subtilis DR2 is a potent organism to be used as biofertilizer. 

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Isolation and characterization of deleterious Pseudomonas aeruginosa KC1 from rhizospheric soils and its interaction with weed seedlings

Cited by 37 publications

References 32 publications

Effect of Ammonia and Indole-3-acetic Acid Producing Endophytic Klebsiella pneumoniae YNA12 as a Bio-Herbicide for Weed Inhibition: Special Reference with Evening Primroses

Effect of Ammonia and Indole-3-acetic Acid Producing Endophytic Klebsiella pneumoniae YNA12 as a Bio-Herbicide for Weed Inhibition: Special Reference with Evening Primroses

Evaluation of Bacteria from Soil and Rhizosphere as Herbicidal Candidates of Some Broadleaf Weeds

Optimization of Indole-3-Acetic Acid Production by Diazotrophic B. subtilis DR2 (KP455653), Isolated from Rhizosphere of Eragrostis cynosuroides

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