Isolation and characterization of Dehalospirillum multivorans gen. nov., sp. nov., a tetrachloroethene-utilizing, strictly anaerobic bacterium

Scholz-Muramatsu, Heidrun; Neumann, Anke; Me�mer, Michael; Moore, Edward R. B.; Diekert, Gabriele

doi:10.1007/bf00262203

Cited by 319 publications

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“…S. multivorans (DSMZ 12446) (19) was grown under anaerobic conditions at 28°C in a pyruvate (40 mM)-and fumarate (40 mM)-containing medium (14). Rubber-stoppered serum glass bottles were used for cultivation.…”

Section: Methodsmentioning

confidence: 99%

“…Rubber-stoppered serum glass bottles were used for cultivation. Unless otherwise stated, anaerobic growth with halogenated ethenes was performed either with formate (40 mM) or pyruvate (40 mM) as an electron donor and PCE (10 mM; see below) as an electron acceptor (19). When the cells were grown with formate, acetate (5 mM) was added as a carbon source.…”

Section: Methodsmentioning

confidence: 99%

“…Concomitantly, the pceA transcript level equally decreased as shown by reverse transcriptase PCR. (19). The organism utilizes pyruvate, hydrogen, or formate as an electron donor.…”

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confidence: 99%

“…Sulfurospirillum multivorans is a strictly anaerobic bacterium capable of respiratory growth with different electron acceptors, namely, fumarate, nitrate, and chlorinated ethenes (tetrachloroethene [perchloroethylene (PCE)] and trichloroethene [TCE]) (19). The organism utilizes pyruvate, hydrogen, or formate as an electron donor.…”

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Retentive Memory of Bacteria: Long-Term Regulation of Dehalorespiration in Sulfurospirillum multivorans

et al. 2009

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The gram-negative, strictly anaerobic epsilonproteobacterium Sulfurospirillum multivorans is able to gain energy from dehalorespiration with tetrachloroethene (perchloroethylene [PCE]) as a terminal electron acceptor. The organism can also utilize fumarate as an electron acceptor. Prolonged subcultivation of S. multivorans in the absence of PCE with pyruvate as an electron donor and fumarate as an electron acceptor resulted in a decrease of PCE dehalogenase (PceA) activity. Concomitantly, the pceA transcript level equally decreased as shown by reverse transcriptase PCR. (19). The organism utilizes pyruvate, hydrogen, or formate as an electron donor. When grown with hydrogen or formate as an electron donor and PCE as an electron acceptor, ATP synthesis is coupled to reductive dechlorination via electron transport phosphorylation (dehalorespiration) (8). The key enzyme of PCE utilization that converts PCE via TCE to cis-1,2-dichloroethene (cDCE) is the PCE reductive dehalogenase (PceA). Mature PceA is a monomeric enzyme harboring one norpseudovitamin B 12 and two Fe/S clusters as cofactors (12,15). The cytoplasmic precursor of the PCE dehalogenase (pre-PceA) bears an N-terminal signal peptide of 37 amino acids, including the motif SRRXFXK, which identifies pre-PceA as a substrate of the Tat (twin arginine translocation) protein export pathway (2). Mature PceA is localized in the periplasm, as shown by freeze fracture immunogold labeling techniques (9).The pce operon comprises pceA, the PCE dehalogenase gene, and pceB, encoding a putative membrane integral protein (16). Genes for transcriptional regulation of the pceAB genes are not found in a 6-kb DNA fragment including the pce operon (GenBank accession number AF022812).The ability of reductive dechlorination by anaerobic bacteria was found in phylogenetically distant species (8). Transcriptional regulation of dehalogenase gene expression was described for the 3-chloro-4-hydroxy-phenylacetate (Cl-OHPA) dehalogenase (CprA) of the gram-positive Desulfitobacterium dehalogenans. In this organism, the enzyme was strictly regulated by the absence or presence of the substrate. When the cultures were grown with alternative electron acceptors, such as fumarate or nitrate, CprA transcription ceased within the first subcultivation and was induced within less than 30 min (21). A CRP/FNR-like transcriptional activator, CprK, encoded within the cpr operon, was shown to interact with the DNA upstream of the cpr promoter region only when Cl-OHPA was bound to the protein (18). A direct role of CprK in the induction of dehalogenase gene expression in D. dehalogenans was assumed, and a model for transcriptional regulation of cprA expression was developed (13).While detailed information is available on the control of dehalogenase gene expression in D. dehalogenans grown in the presence of chlorinated aromatic compounds, such as Cl-OHPA, a transcriptional regulation of the PCE dehalogenase genes of Desulfitobacterium species has not been described so far (23). Tsukagoshi and coworker...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Concomitantly, the pceA transcript level equally decreased as shown by reverse transcriptase PCR. (19). The organism utilizes pyruvate, hydrogen, or formate as an electron donor.…”

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confidence: 99%

mentioning

confidence: 99%

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Retentive Memory of Bacteria: Long-Term Regulation of Dehalorespiration in Sulfurospirillum multivorans

et al. 2009

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“…Previous laboratory studies found that the initial degradation of these compounds, often a dehalogenation, can be carried out by speci®c halorespiring bacteria (Deweerd et al, 1990;Scholtz-Muramatsu et al, 1995;Utkin et al, 1995). Because the products of reductive dehalogenation are generally less toxic, less likely to bioaccumulate, and more susceptible to further degradation, reductive transformations are important for Chemosphere 44 (2001) 1557±1563 www.elsevier.com/locate/chemosphere decreasing the risks associated with the presence of halogenated organic hydrocarbons in the environment (Zitomer and Speece, 1993).…”

Section: Introductionmentioning

confidence: 99%

Quantitative structure–property relationship study on reductive dehalogenation of selected halogenated aliphatic hydrocarbons in sediment slurries

et al. 2001

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Reductive Dehalogenation and Metabolism of Haloorganics

Zinder

2003

Encyclopedia of Environmental Microbiology

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Introduction Cometabolic Reductive Dechlorination Reductive Dechlorination and Dehalorespiration Chloroethene Utilization Chlorinated Methane and Trichloroacetate Utilization Chlorophenol and Bromophenol Utilization Reductive Dehalogenation of other Organohalogen Compounds by Mixed Microbial Populations Biochemistry and Molecular Biology of Reductive Dechlorination Bioremediation Studies

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Isolation and characterization of Dehalospirillum multivorans gen. nov., sp. nov., a tetrachloroethene-utilizing, strictly anaerobic bacterium

Cited by 319 publications

References 18 publications

Retentive Memory of Bacteria: Long-Term Regulation of Dehalorespiration in Sulfurospirillum multivorans

Retentive Memory of Bacteria: Long-Term Regulation of Dehalorespiration in Sulfurospirillum multivorans

Quantitative structure–property relationship study on reductive dehalogenation of selected halogenated aliphatic hydrocarbons in sediment slurries

Reductive Dehalogenation and Metabolism of Haloorganics

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