Isolation and Characterization of Cytotoxic Principles from Hyptis verticillata Jacq

The Bioassay-Guided Isolation of Growth Inhibitors of Adult T-Cell Leukemia (ATL), from the Jamaican Plant Hyptis verticillata, and NMR Characterization of Hyptoside

“…β-Peltatin ( 3 ): colorless amorphous solid; [α] 25 D −132.5 ( c 0.05, CH 2 Cl 2 ); UV λ max (CH 2 Cl 2 ) 274 (2.892); IR (film): 2935, 1764, 1588, 1463, 1248, 1124 cm −1 : MS and NMR data identical to those reported [ 12 ].…”

“…On the basis of this information, the structure of 1 was identified as 5-methoxy hyptinin and its 13 C-NMR data were assigned by the HMQC and HMBC correlations, as shown in Table 1 . In addition, hyptinin ( 2 ) [ 7 ], β -peltatin ( 3 ) [ 12 ], 4′-demethyldesoxypodophyllotoxin ( 4 ) [ 13 ] and deoxypicropodophyllin ( 5 ) [ 14 ] were identified by comparison with data from the literature. The absolute stereochemistry of compounds 1 and 2 remains to be determined, as attempts at hydrogenation were unsuccessful due to the paucity of sample obtained; signs of optical rotation for these compounds, however, were not identical to those reported for hyptinin.…”

“…In the present study, the in vitro inhibitory effects on proliferation and the induction of apoptosis in S1T leukemia cells by the methanol extract of the dried aerial parts of the Jamaican H. verticillata are demonstrated. Also described are the bioassay-guided isolation, the structural elucidation and inhibitory activity against S1T cells of five lignans: hyptoside ( 1 ) [ 11 ], hyptinin ( 2 ) [ 7 ], β -peltatin ( 3 ) [ 12 ], 4′-demethyldesoxypodophyllotoxin ( 4 ) [ 13 ], and deoxypicropodophyllin ( 5 ) [ 14 ]. This is the first bioassay-guided isolation of anti-ATL compounds from plant extracts.…”

Investigation of Podophyllotoxin in some Plants in Lamiaceae Using HPLC: Türkiye'de Yetişen Lamiaceae Familyasındaki Bazı Türlerde Podofilotoksin'in Yüksek Basınçlı Sıvı Kromatografisi ile Tespiti

“…Lamiaceae is a family of herbs or shrubs usually glandular and aromatic, comprising about 400 species divided into 45 genera in Turkey. Only three phytochemical studies dealing with lignans content of Hyptis tomentosa and H. verticillata have been reported to date (7)(8)(9).…”

Abietane Diterpeniods fromHyptis Verticillata

“…Royleanone (5) and 7-acetoxyhorminone (7) have been reported to exhibit cytotoxic and DNA damaging effects on colonic and hepatic human cells [13]. Royleanone (5) was also reported to exhibited cytotoxicity against human carcinoma of the nasopharynx [14].…”