Isolation and characterization of cytochromeb-559 from chloroplasts and etioplasts of barley

Koenig, Friederike; Møller, Birger Lindberg

doi:10.1007/bf02907786

Cited by 17 publications

(3 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Electrophoretic analysis of isolated cytochrome b-559 revealed the presence of a Coomassie Blue staining band at M r 9,300. Bands staining with 3,3',5,5'-tetramethyl benzidine (TMBZ) but not staining with Coomassie Blue were observed at M r 32,000 and 31,000 (28). The here isolated M r 32,000 polypeptide did not stain with TMBZ.…”

Section: Characteristics and Function Of The Isolated M R 32000 Polymentioning

confidence: 89%

A thylakoid polypeptide involved in the reconstitution of photosynthetic oxygen evolution

Møller

Høj

1983

Carlsberg Res. Commun.

Self Cite

View full text Add to dashboard Cite

Sonication of barley thylakoids in a high salt buffer released three polypeptides of M r 32,000, 23,000, and 13,500 which were purified to homogeneity by chromatofocusing.Highly purified inside-out photosystem II preparations were obtained by French Press treatment or by Triton X-100 fractionation of stacked lamellar systems. Both preparations are composed of pairs of appressed membrane sheets. In the French Press preparation, the majority of these membrane pairs are sealed whereas they are predominantly unsealed in the Triton X-100 preparation. Both preparations are able to evolve oxygen and show reversed proton pumping. The oxygen evolving capacity and variable fluorescence of both preparations were lost upon washing with high salt buffer. This treatment also removed three polypeptides at M r 32,000, 23,000, and 13,500. The inactivated preparations were reconstituted with respect to oxygen evolution and variable fluorescence by rebinding of the isolated M r 23,000 polypeptide. The M r 32,000 and 13,500 polypeptides had no effect on reconstitution.Abbreviations: Chl = chlorophyll; chl-P = chlorophyll-protein; DCCD = dicyclohexyl carbodiimide: DCMU = 3-(3,4-dichlorophenyl)-l,l-dimethylurea; DCPIP = 2,6-dichlorophenol indophenol; EPR = electron paramagnetic resonance; Hepes = N-2-hydroxyethylpiperazine-N'-2-ethane sulfonic acid; PPBQ = phenyl-p-benzoquinone: PS = photosystem; SDS-PAGE = sodium dodecylsulfate polyacrylamide gel electrophoresis; TMBZ = 3.Y,5,5'-tetramethylbenzidine; Tricine = N-(2-hydroxy-l,l-bis(hydroxymethyl)ethyl)glycine: Tris = tris-(hydroxymethyl)-amino methane 0105/1938/83/0048/0161/$05.00

show abstract

Section: Characteristics and Function Of The Isolated M R 32000 Polymentioning

confidence: 89%

A thylakoid polypeptide involved in the reconstitution of photosynthetic oxygen evolution

Møller

Høj

1983

Carlsberg Res. Commun.

Self Cite

View full text Add to dashboard Cite

show abstract

“…KRUPINSKA & S. BERRV-LowE: Cytochrome b-559 genes lakoid membranes as a lipoprotein complex with an apparent molecular weight of 110 kD (28). Dissociation of the complex under mild conditions yields subunits of about 30 kD that still contain heme (15). After complete denaturation of the complex a 9-10 kD polypeptide is obtained and this polypeptide can be synthesized in isolated chloroplasts (32).…”

Section: Introductionmentioning

confidence: 99%

“…Models of homodimers or heterodimers as the smallest heme containing subunits of cytochrome b-559 have been proposed (12). However, these models hardly give an understanding of the molecular organization of the 110 kD complex containing only one heme molecule per protein moiety of 30-46 kD (15,28).…”

Section: Introductionmentioning

confidence: 99%

Characterization and in vitro expression of the cytochromeb-559 genes of barley I. Localization and sequence of the genes

Krupinska

Berry-Lowe

1988

Carlsberg Res. Commun.

View full text Add to dashboard Cite

The psbE and psbF genes encoding the 9.4 and 4.4 kD apoproteins of cytochrome b-559 have been located in the chloroplast genome of barley. As in other plant species they are found adjacent to each other in the large single copy region of the chloroplast DNA. Both the nucleotide sequence and the deduced amino acid sequence for the two polypeptides are identical to that of wheat and more than 95% similar to those of spinach, tobacco and Oenothera. The region between the two genes spans 10 nucleotides (excluding the stopcodon) and contains a typical procaryotic ribosomal binding site. A dicistronic transcript is identified, but the presence of a ribosomal binding site between the two genes may allow independent translation.

show abstract

Molecular Genetics of Photosystems I and II

Wettstein

1984

Advances in Photosynthesis Research

View full text Add to dashboard Cite

Isolation and characterization of cytochromeb-559 from chloroplasts and etioplasts of barley

Cited by 17 publications

References 50 publications

A thylakoid polypeptide involved in the reconstitution of photosynthetic oxygen evolution

A thylakoid polypeptide involved in the reconstitution of photosynthetic oxygen evolution

Characterization and in vitro expression of the cytochromeb-559 genes of barley I. Localization and sequence of the genes

Molecular Genetics of Photosystems I and II

Contact Info

Product

Resources

About