Isolation and Characterization of Chemopreventive agent from Sphaeranthus amaranthoides Burm F

Abstract

Objective:To investigate the in vitro cytotoxic effect and to isolate and characterize a chemopreventive secondary metabolite from Sphaeranthus amaranthoides Burm F (sivakaranthai).Materials and Methods:In vitro cytotoxic effect was carried out by 3 (4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide assay. Different concentrations of the extracts were tested on three different cell lines namely A549, HT29, and MCF7. The chloroform extract was subjected to column chromatography, and the isolated compound was characterized by various spectral methods and by single crystal X-ray crystallography.Results:The concentration that cause 50% growth inhibition value of chloroform extract was found to be 0.9 and 19 μg/mL against MCF7 and A549 cell lines, respectively. Chloroform extract was subjected to column chromatography for the isolation of phytoconstituent. The structure of the isolated compound was identified by spectroscopic techniques such as infrared, nuclear magnetic resonance, XRD, and mass spectroscopy. On comparison of complete spectral detail of the compound, the proposed structure was identified as chrysosplenol D (a flavonoid). Chrysosplenol D was isolated for the first time from this plant.Conclusion:The chloroform extract had higher cytotoxic effect, and the isolated chrysosplenol D may be responsible for the anti-proliferative effect of the plant.SUMMARY The plant Sphaeranthus amaranthoides Burm F was extracted with solvents of increasing polarity. The chloroform extract was found to have cell inhibition towards MCF 7 and HT 29 cell lines. This was subjected to fractionation. Chrysosplenol D was isolated from the chloroform extract

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“…The methanolic extract of sivakaranthai whole plant, collected in the 'normal day' has also been subjected to HPLC analysis and three major peaks with masses of MW: 516Da, MW: 360Da and MW: 374Da were identified. The peak with mass 360Da has been identified and reported as a chemopreventive agent, namely, chrysosplenol D [7] by Gayatri et al (2016). Further, the peak with MW: 374Da identified by us has the mass difference of 14 units with the reported one and this difference is due to the one additional methyl group leading to the prediction of the structure for the MW 374 to be the one shown in Fig.…”

Biological Activity of Cyclic Peptide Extracted from Sphaeranthus amaranthoides Using De Novo Sequencing Strategy by Mass Spectrometry for Cancer

“…The chloroform extracts of S. amaranthoides have shown cytotoxicity and anti-tumour effects. In addition, chrysosplenol D, a flavonoid, was identified from S. amaranthoides with a chemoprotective effect [ 14 ]. However, there are no details regarding the AMPs from S. amaranthoides .…”