Isolation and characterization of cDNAs encoding storage proteins of chickpea (Cicer arietinum L.)

Functional characterization of an endosperm specific promoter p1062 from common buckwheat (Fagopyrum esculentum Moench) for driving tissue specific gene expression / Funkcijske lastnosti endospermskega promotorja p1062 navadne ajde ...

“…With the potentiality of genetic engineering being amply demon-INTRODUCTION strated a first step towards production of transgenic plants with improved amino acid composition entails the purification and characterization of the specific seed storage protein rich in essential amino acids followed by cloning a full length gene coding for the target protein. Such proteins and their genes have been isolated from soyabean (Hill & Breidenbach 1974), pea (Higgins et al 1986;Hoffman et al 1988), Lupinus albus (Melo et al 1994), rice (Takaiwa et al 1987;Krishnan & Pueppke 1993), oat (Shotwell et al 1990), Phaseolus vulgaris (Goossens et al 1994), rape seed (Coupe et al 1993); field bean (Heim et al 1994); chickpea (Khitha et al 1995;Mandaokar, & Koundal 1996;Saha & Koundal 1998); Chenopodium (Dey et al 1993); Brassica (Dasgupta & Mandal 1991;Utsumi et al 1993;Dasgupta et al 1995); grain amaranth (Raina & Datta 1992), buckwheat (Bharali, & Chrungoo 2003). Due to the balanced amino acid composition, high nutrient value and homology with seed storage proteins of leguminous group of plants, the nutritionally rich component of protein of common buckwheat (Fagopyrum esculentum Moench) which is a 26kDa basic subunit, having more than 6% lysine and nearly 2% methionine (Bharali, & Chrungoo 2003) could be an important candidate for compensation of limiting amino acid in plants deficient in such amino acids.…”