Isolation and Characterization of cDNA Clones Encoding ADP-glucose Pyrophosphorylase from Sago Palm ( Metroxylon sagu ) *

Abstract

Four ADP-glucose pyrophosphorylase cDNA clones were isolated from mature leaves and pith of sago palm by the polymerase chain reaction (PCR) technique. Three of them (agpp10, agpp12 and agpl19) encoded the AGP large subunit, while the fourth clone (agpl1) encoded the small subunit. agpp10 and agpp12 were isolated from pith, agpl19 was isolated from mature leaves, while agpl1 from both tissues. In addition, a full-length cDNA of agpl1 was successfully isolated from a cDNA library of mature leaves by a PCR-based screening technique. Semi-quantitative analysis suggests that agpp10 and agpp12 were detectable only in pith, agpl19 only in leaves, while agpl1 was expressed in both leaves and pith tissues.

The AGPase Family Proteins in Banana: Genome-Wide Identification, Phylogeny, and Expression Analyses Reveal Their Involvement in the Development, Ripening, and Abiotic/Biotic Stress Responses

“…In contrast, the APS is regulatory-deficient but it plays more of a catalytic role in plant tissues [ 15 ]. To date, genome-wide analysis has identified a variable number of APL and APS in numerous higher plants, including six in A. thaliana (four APLs and two APSs ) [ 15 ], seven in rice ( Oryza sativa ) (four APLs and three APSs ) [ 16 , 17 ], six in maize ( Zea mays ) (four APLs and two APSs ) [ 13 , 18 ], six in sweet potato ( Ipomoea batatas ) (four APLs and two APSs ) [ 19 ], four in sago palm ( Metroxylon sagu ) (three APLs and one APS ) [ 20 ], four in kiwifruit ( Actinidia deliciosa ) (three APLs and one APS ) [ 21 ], and four in tomato ( Solanum lycopersicon ) (three APLs and one APS ) [ 22 ].…”