Isolation and characterization of cDNA clones encoding a 32-kDa dense-granule antigen of Sarcocystis muris (Apicomplexa)

Freyer, Bernd; Eschenbacher, Karl Heinz; Mehlhorn, Heinz; Rueger, Wolfgang

doi:10.1007/s004360050453

Cited by 9 publications

(2 citation statements)

References 43 publications

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“…Each of these is found throughout the Plasmodium genus and obvious orthologues are not present in any other organism. We note, however, that reciprocal BLAST analysis suggests that EXP2 is related to the dense granule family of secreted proteins known as DG32 in other Apicomplexan parasites22, although the relatively low level of identity and absence of conserved cysteine residues means it is unclear if DG32 proteins represent orthologs of EXP2. Hence, the PTEX complex is a novel PVM-associated, ATPase machine that satisfies the first four essential criteria for a PEXEL-protein translocon.…”

Section: Identification Of a Novel 5 Member Putative Translocon Complmentioning

confidence: 84%

A newly discovered protein export machine in malaria parasites

Koning-Ward

Gilson

Boddey

et al. 2009

Nature

445

616

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Several hundred malaria parasite proteins are exported beyond an encasing vacuole and into the cytosol of the host erythrocyte, a process that is key to the virulence and viability of the causative Plasmodium species. The trafficking machinery responsible for this export is unknown. Here, we identify a Plasmodium Translocon of EXported proteins (PTEX), which is located in the vacuole membrane. The PTEX complex is ATP-powered and comprises HSP101, which is a ClpA/B-like AAA+ ATPase of a type commonly associated with protein translocons, a novel protein termed PTEX150 and a known parasite protein EXP2. EXP2 is the potential channel as it is the membrane-associated component of the core PTEX complex. Two other proteins, a novel protein PTEX88 and a thioredoxin known as TRX2, were also identified as PTEX components. As a common portal for numerous crucial processes, this novel translocon offers an exciting new avenue for therapeutic intervention.

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Section: Identification Of a Novel 5 Member Putative Translocon Complmentioning

confidence: 84%

A newly discovered protein export machine in malaria parasites

Koning-Ward

Gilson

Boddey

et al. 2009

Nature

445

616

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“…The mammalian lysosomal PPT1 enzyme has a putative homologue in T. gondii (TGME49_097880). This protein is expected to be a dense granule protein based on similarity to a Sarcocystis muris dense granule antigen (64,65). It shares only 12% identity and 40% similarity with the mammalian PPT1 and has no predicted serine hydrolase activity based on its sequence.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Serine Hydrolase Targeted by Acyl-protein Thioesterase Inhibitors in Toxoplasma gondii

Kemp

Rusch²,

Adibekian

et al. 2013

Journal of Biological Chemistry

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Background: S-Palmitoylation is an important reversible modification that involves the action of an acyl-protein thioesterase (APT). Results:We identified an active serine hydrolase (TgASH1) specifically targeted by human APT1 inhibitors in Toxoplasma gondii. Conclusion: TgASH1 is dispensable and cannot be solely responsible for S-depalmitoylation in Apicomplexa. Significance: ␤-Lactone-based APT1 inhibitors hit multiple targets in T. gondii and severely compromise parasite survival.

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Sarcosporidiosis

Mehlhorn

2011

Encyclopedia of Life Sciences

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Sarcosporidiosis is a disease that occurs worldwide in many animals and human beings as a result of oral infections with developmental stages of several Sarcocystis species, the life cycle of which includes two types of hosts: a prey and a predator. Humans may be involved in both groups. The prey hosts are herbi‐ or omnivores, which are infected by oral uptake of oocysts and sporocysts from the feces of the predator hosts, which are carnivorous beings including man. The specimens of the latter group are infected by eating raw or undercooked meat of the prey animals containing so‐called cyst merozoites (cystozoites) inside tissue cyst in muscle cells or in brain cells. The final hosts (=carnivores with the sexual development inside their intestinal cells) may suffer from diarrhoea in case of severe infections. The intermediate hosts (=prey hosts) may severely suffer from bleeding of inner blood vessels (where the schizonts and merozoites develop within the first month after infection) and by destruction of brain and muscle cells, where the cysts are formed. After about 3 months the cyst merozoites inside the tissue cysts are infectious for the final host. Key Concepts: Human intestinal infections with Sarcocystis species are completely prohibited by noneating raw or undercooked meat. Strict separation of human, dog and cat feces from food of farm animals prohibits their infections with Sarcocystis species. Sarcosporidiosis is completey avoidable on farms by strict hygienic measurements. Deep freezing or cooking of meat will kill sarcosporidians.

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Isolation and characterization of cDNA clones encoding a 32-kDa dense-granule antigen of Sarcocystis muris (Apicomplexa)

Cited by 9 publications

References 43 publications

A newly discovered protein export machine in malaria parasites

A newly discovered protein export machine in malaria parasites

Characterization of a Serine Hydrolase Targeted by Acyl-protein Thioesterase Inhibitors in Toxoplasma gondii

Sarcosporidiosis

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