Isolation and characterization of Borrelia burgdorferi — specific and autoreactive T cell lines from the cerebrospinal fluid of patients with lyme meningoradiculitis

Martin, Roland; Ortlauf, Johannes; Sticht-Groh, V.; Mertens, H. G.

doi:10.1016/0165-5728(87)90306-7

Cited by 6 publications

(7 citation statements)

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“…Although comparable studies have not been reported so far for mice or other rodents, B. burgdorferispecific T cells have been isolated from humans suffering from Lyme disease (26). In particular, T cells specific for spirochete antigens have been obtained from specimens derived from inflamed organ sites, such as joint exudate during Lyme arthritis (M. Schlesier, personal communication) and cerebrospinal fluid during B. burgdorferi-induced meningoradiculitis (19). The combined data therefore clearly show that T cells with specificity for B. burgdorferi are generated in humans and mice after infection or inoculation with the spirochete.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, the T-cell response to B. burgdorferi is poorly defined. Previous studies of the cellular immunity in human Lyme disease indicated the presence in antigen-specific T lymphocytes in patients expressing certain stages of the illness (19,26). In light of the ever-increasing incidence of infections with B. burgdorferi and the multisystemic nature of the disease, which may also involve immunopathogenetic mechanisms, more detailed analysis of the T-cell response is required.…”

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confidence: 99%

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Demonstration of antigen-specific T cells and histopathological alterations in mice experimentally inoculated with Borrelia burgdorferi

et al. 1989

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Antigen-specific T-ceH responses and histopathological changes were studied in mice experimentally inoculated with Borrelia burgdorferi B31. Inbred mice with different H-2 haplotypes and/or different genetic backgrounds were inoculated with B. burgdorferi organisms and tested for antigen-specific T-cell responses in vivo (delayed-type hypersensitivity [DTH]) and in vitro (T-cell proliferation). Comparable DTH responses were found after inoculation with either inactivated (in the presence of adjuvants) or viable microorganisms in all mouse strains, except BALB/c, irrespective of the H-2 haplotype (b, d, k, or s) tested and the sex of the animals.Moreover, in mice presensitized to B. burgdorferi, DTH responses could be induced only with antigen preparations derived from the corresponding strain but not with those obtained from either related spirochetes such as Treponema phagedenis and Leptospira interrogans or unrelated bacteria such as Mycobacterium tuberculosis. T cells isolated from lymph nodes or spleens of mice previously sensitized to B. burgdorferi but not those from naive mice could be induced for antigen-specific proliferation in vitro, as revealed by [3H]thymidine incorporation. Histopathological examination of mice inoculated with viable B. burgdorferi organisms revealed significant perivascular inifitrates consisting mainly of mononuclear and a few polymorphonuclear leukocytes in different organs (brain, heart, lungs, liver, and kidneys) and the appearance of giant multinucleated cells within the spleen similar to those found in human skin specimens of patients suffering from cutaneous manifestations of Lyme disease. Our findings suggest that mice are a suitable animal model with which to study the immune response to B. burgdorferi and the pathogenesis of Lyme disease.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Demonstration of antigen-specific T cells and histopathological alterations in mice experimentally inoculated with Borrelia burgdorferi

et al. 1989

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“…1,1988 Investigations of cell-mediated immunity showed a specific response by T cells of Lyme borreliosis patients to B. burgdorferi antigens (57,124,132,152). This response has been demonstrated for T cells obtained from the peripheral blood, the diseased synovium, and the CSF.…”

Section: Differential Diagnosismentioning

confidence: 99%

“…Antigen-specific T-cell responses have also been demonstrated in the synovial fluids of patients with postinfectious Reiter's syndrome (66). T-cell clones that respond specifically to B. burgdorferi antigens have been recovered from the CSF of a patient with Lyme borreliosis (124 (152). The T-cell responses of patients show less apparent cross-reactivities than the corresponding antibody responses to relapsing fever borreliae antigens (152).…”

Section: Differential Diagnosismentioning

confidence: 99%

Laboratory aspects of Lyme borreliosis

Barbour

1988

Clin Microbiol Rev

129

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Lyme borreliosis (Lyme disease), a common tick-borne disorder of people and domestic animals in North America and Europe, is caused by the spirochete Borrelia burgdorferi. Following the discovery and initial propagation of this agent in 1981 came revelations that other tick-associated infectious disorders are but different forms of Lyme borreliosis. A challenge for the clinician and microbiology laboratory is confirmation that a skin rash, a chronic meningitis, an episode of myocarditis, or an arthritic joint is the consequence of B. burgdorferi infection. The diagnosis of Lyme borreliosis may be established by (i) directly observing the spirochete in host fluid or tissue, (ii) recovering the etiologic spirochete from the patient in culture medium or indirectly through inoculation of laboratory animals, or (iii) carrying out serologic tests with the patient's serum or cerebrospinal fluid. The last method, while lacking in discriminatory power, is the most efficacious diagnostic assay for most laboratories at present.

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“…In contrast, the T cell-mediated response to B. burgdorferi is poorly defined (4,21). Previous studies of cellular immunity in patients with Lyme disease indicated the presence of antigen-specific T cells at certain stages of the illness (18 In all cases, culture results were confirmed by PCR. We observed differences in infectivity between strains of mice and between Borrelia spirochetes.…”

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confidence: 88%

T Cell Response to Borrelia garinii, Borrelia afzelii, and Borrelia japonica in Various Congenic Mouse Strains

Ishii

Isogai

et al. 1995

Microbiology and Immunology

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The infectivity and T cell response to Borrelia garinii SIKA2, Borrelia afzelii BFOX, and Borrelia japonica 0612, the organisms that cause Lyme disease in Japan, were examined in various inbred and congenic strains of mice. Infectivity differed among the species: B. garinii SIKA2 and B. afzelii BFOX were each able to infect 90% to 100% of C3H/He mice; B. japonica 0612 was able to infect only 20% of C3H/He mice. The pattern of infectivity to various inbred and congenic strains of mice may influence the pathogenicity of the organism and the clinical signs of Lyme disease. Cross-reactivity between Borrelia antigens was observed, but there was no cross-reactivity between Borrelia antigens and Leptospira antigens. We evaluated the genetic control of the delayed-type hypersensitivity (DTH) reaction in the form of footpad swelling produced by Borrelia antigens using viable or sonicated bacteria as sensitization. Differences in strains of mice infected by viable antigen were observed. However, all strains of mice showed a strong DTH reaction using sonicated antigens without genetic background. A DTH reaction in the form of footpad swelling did not appear to be associated with genetic background. The footpad reaction was mediated by CD4+8-and Ia-T cells, as revealed by in vitro monoclonal antibody treatment. However, CD8+ T cells did not suppress footpad swelling. These results indicate that many antigenic epitopes of the Borrelia spirochete can stimulate the DTH reaction.

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Isolation and characterization of Borrelia burgdorferi — specific and autoreactive T cell lines from the cerebrospinal fluid of patients with lyme meningoradiculitis

Cited by 6 publications

References 0 publications

Demonstration of antigen-specific T cells and histopathological alterations in mice experimentally inoculated with Borrelia burgdorferi

Demonstration of antigen-specific T cells and histopathological alterations in mice experimentally inoculated with Borrelia burgdorferi

Laboratory aspects of Lyme borreliosis

T Cell Response to Borrelia garinii, Borrelia afzelii, and Borrelia japonica in Various Congenic Mouse Strains

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