Isolation and characterization of blastocystis spp. isolated from anuran amphibians

Yoshikawa, Hideki; Morimoto, Keiko; Nagashima, M

doi:10.1016/s1383-5769(98)80853-2

Cited by 4 publications

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“…Recently, the genetic diversity among B. hominis strains has been ascertained by using different techniques (2,4,6,12). Although at present the name Blastocystis hominis is used for strains isolated from humans, many B. hominis-like organisms were isolated from a wide variety of animals (1,14,15). Since these organisms were indistinguishable from B. hominis by morphological criteria, it is difficult to judge whether B. hominis-like organisms isolated from various animals are identical to or different from B. hominis.…”

Section: Discussionmentioning

confidence: 99%

“…Current thought describes B. hominis as organisms that have been isolated from humans. However, many B. hominis-like organisms have been isolated from a wide range of animals, such as nonhuman primates, as well as rodents, birds, amphibians, reptiles, and insects (1,14,15). Although most of these organisms were indistinguishable from B. hominis based on light and electron microscopy, genetic diversity among B. hominis strains has been demonstrated (2,6,12).…”

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Genomic Analysis of Blastocystis hominis Strains Isolated from Two Long-Term Health Care Facilities

et al. 2000

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The genotype Blastocystis hominis is highly polymorphic. Therefore, a genetic marker would be a powerful tool for the identification or classification of B. hominis subtypes and could be used as a means to resolve the transmission route or origin of the parasite. To this end, 32 B. hominis isolates were collected from patients and/or staff members of two long-term health care facilities (facilities A and B), and these organisms were subjected to genotype analysis based on diagnostic PCR primers and restriction fragment length polymorphism (RFLP) of small subunit rRNA gene (rDNA). Based on PCR amplification using diagnostic primers which were developed from randomly amplified polymorphic DNA analysis of known strains of B. hominis, the 32 isolates of B. hominis were classified into three different subtypes. Thirty isolates, including twenty-four that were isolated from patients and a staff member, from facility A and all isolates isolated from six patients from facility B showed the same genotype. Two of six patients of facility B had been transferred from facility A, and these two patients also had the same-genotype B. hominis that corresponded to 24 isolates from facility A. This genotype strain may have been transmitted by these two patients from facility A to facility B, suggesting human-to-human transmission. In contrast, 2 of 26 isolates from facility A showed distinct genotypes, suggesting that the colonization by these two isolates is attributable to another infectious route. These different subtypes were subjected to RFLP analysis, and the RFLP profiles were correlated with the results obtained by diagnostic PCR primers. This study presents the first molecular evidence of possible human-to-human B. hominisinfection between and/or among two small communities.

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Genomic Analysis of Blastocystis hominis Strains Isolated from Two Long-Term Health Care Facilities

et al. 2000

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Molecular characterization of Blastocystis isolates from birds by PCR with diagnostic primers and restriction fragment length polymorphism analysis of the small subunit ribosomal RNA gene

Abe

Yoshikawa

2003

Parasitol Res

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Seven Blastocystis isolates from birds were genotyped by polymerase chain reaction (PCR) using diagnostic primers, and the homology among isolates was then confirmed by restriction fragment length polymorphism (RFLP) analysis of the small subunit ribosomal RNA gene. A zoonotic genotype, subtype 4, was detected in three isolates by PCR, but the RFLP profiles of these isolates differed from those of the subtype 4 reported previously from a human. None of the other four isolates were amplified with any subtype-specific primer sets, and the profiles of these isolates were different from any ribodemes of Blastocystis hominis reported previously. In the present study, the seven isolates from birds were classified into three genetically distinct genotypes. Therefore, birds appear to be infected with multiple genotypes. It is unclear whether the isolates examined in the present study are zoonotic or not. Therefore, it is important to determine the phylogenetic relationships between unknown genotypes from birds and the multiple genotypes of B. hominis.

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Zoonotic genotypes of Blastocystis hominis detected in cattle and pigs by PCR with diagnostic primers and restriction fragment length polymorphism analysis of the small subunit ribosomal RNA gene

Abe

Yoshikawa

2003

Parasitol Res

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Twenty two Blastocystis isolates from cattle and pigs were genotyped by PCR using diagnostic primers, and the homology among isolates was then confirmed by restriction fragment length polymorphism (RFLP) analysis of the small subunit ribosomal RNA gene. Two distinct genotypes, subtypes 1 and 3, were detected in one and two of the ten isolates from cattle, and three and one of the 12 isolates from pigs, respectively. The RFLP profiles of all isolates designated as subtype 1 and subtype 3 were identical to those of known ribodemes 1 and 2, respectively. We found that 31.8% (7/22) of isolates examined were zoonotic genotypes of Blastocystis hominis. However, 68.2% (15/22) of isolates were not amplified with any of the diagnostic primers, and the profiles of these isolates were different from any ribodemes of B. hominis reported previously. This study shows that Blastocystis organisms in cattle and pigs are a potential source of human infection.

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Isolation and characterization of blastocystis spp. isolated from anuran amphibians

Cited by 4 publications

References 0 publications

Genomic Analysis of Blastocystis hominis Strains Isolated from Two Long-Term Health Care Facilities

Genomic Analysis of Blastocystis hominis Strains Isolated from Two Long-Term Health Care Facilities

Molecular characterization of Blastocystis isolates from birds by PCR with diagnostic primers and restriction fragment length polymorphism analysis of the small subunit ribosomal RNA gene

Zoonotic genotypes of Blastocystis hominis detected in cattle and pigs by PCR with diagnostic primers and restriction fragment length polymorphism analysis of the small subunit ribosomal RNA gene

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