Isolation and Characterization of Bioactive Metabolites from Fruiting Bodies and Mycelial Culture of Ganoderma oerstedii (Higher Basidiomycetes) from Mexico

Abstract

Various species of the genus Ganoderma have been used for centuries according to oriental tradition as a source of medicines and nutrients. A chemical study of the fruiting bodies and mycelial culture of G. oerstedii was carried out with the idea of isolating and characterizing active natural components present to make use of their potential pharmaceutical application in Mexico. The fruiting bodies and mycelial culture of G. oesrtedii were lyophylized and extracted one after the other with hexane, chloroform, and methanol. Following this process, each substance was extracted separately by using column chromatography. From fruiting bodies eight metabolites, five sterols (ergosta-7,22-dien-3β-ol, ergosterol peroxide, ergosterol, cerevisterol, and ergosta-7,22-dien-3-one) as well as three terpene compounds (ganodermanondiol, ganoderic acid Sz, and ganoderitriol M) were obtained from fruiting bodies. From the mycelial culture three metabolites, two sterols (ergosterol and cerevisterol), and a new terpene compound (ganoderic acetate from the acid) were obtained. These structures were established based on a spectroscopic analysis mainly using nuclear magnetic resonance and a comparison with data already established.

Brefeldin-A: an Antiproliferative Metabolite of the Fungus Curvularia trifolii Collected from the Veracruz Coral Reef System, Mexico

“…All the compounds were identified by comparison of their 1 H and 13 C NMR spectra with data previously reported in the literature. The resulting products were disclosed as ergosterol (1) [9], ergosterol peroxide (2) [9], cerevisterol (3) [10] and brefeldin-A (4) [11]. The fraction which presented higher values of antiproliferative activity (A549, SW1573, HeLa, HBL-100, T-47D, and WiDr with GI 50 1.2, 1.4, 0.69, 1.4, 1.0, and 0.46 µg/mL, respectively) corresponded to brefeldin A (4).…”

Micoquímica en México: Breve reseña histórica

“…Así, Suárez-Medellín et al (2014), aislaron ergosterol, acetato de ergosterol, ergosta-7,22-dien-3β-ol, peróxido de ergosterol, ergosta-7,22-dien-3-ona, cerevisterol, ácido ganodérico DM, lucidumol A, ganoderitriol M y ganoderiol D a partir de los basidiocarpos silvestres de una cepa mexicana de G. lucidum. Por su parte, Mendoza et al (2015) estudiaron los carpóforos silvestres y el micelio cultivado en medio líquido de G. oerstedii. A partir de los cuerpos fructíferos aislaron cinco esteroles (ergosta-7,22-dien-3β-ol, peróxido de ergosterol, ergosterol, cerevisterol, y ergosta-7,22-dien-3-ona) y tres triterpenoides (ganodermanondiol, ácido ganodérico Sz y ganoderitriol M), mientras que a partir del micelio cultivado obtuvieron ergosterol, cerevisterol y acetato de ácido ganodérico Y.…”

Study of the Lipolysis Effect of Nanoliposome-Encapsulated Ganoderma lucidum Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach

“…Ganoderma species are generally found all over the world. Lingzhi exhibits the prevailing features of being an excellent nutrient source of proteins, lipids, and carbohydrates [ 5 ]. Lingzhi has been consumed widely in East-Asia as a traditional remedy for centuries [ 6 ].…”