Isolation and characterization of bioactive lactoferrin from camel milk by novel pH-dependent method for large scale production

Mahala, Neelam; Mittal, Aastha; Lal, Madan; Dubey, Uma S.

doi:10.1016/j.btre.2022.e00765

Cited by 8 publications

(2 citation statements)

References 38 publications

(39 reference statements)

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“…The methodology is followed from our previously reported work. [26,27] Briefly, HEK 293 cells were harvested, counted, and seeded at 7000 cells per well in the 96-well plate for 24 h. The following day, cells were treated with various concentrations of the sorafenib-loaded casein nanoparticles and free sorafenib for 24 h. Untreated cells served as a negative control. Subsequently, 20 µL of 5 mg mL −1 MTT reagent was added to each well and incubated for 4 h. Lastly, the media was removed, and 150 µL of DMSO was added to the wells to solubilize the formazan crystals.…”

Section: Cell Viability Assaymentioning

confidence: 99%

Evaluation of the cytotoxic activity of sorafenib‐loaded camel milk casein nanoparticles against hepatocarcinoma cells

Mittal,

Mahala,

Dhanawade

et al. 2024

Biotechnology Journal

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Sorafenib, a multikinase inhibitor is used to treat hepatocellular and renal carcinoma. However, a low solubility impedes its bioavailability and thus, effectiveness. This study aims to enhance its effectiveness by using novel camel milk casein nanoparticles as a delivery system. This study evaluates the cytotoxicity of sorafenib encapsulated in camel milk casein nanoparticles against human hepatocarcinoma cells (HepG2 cells) in vitro. Optimal drug loaded nanoparticles were stable for 1 month, had encapsulation efficiency of 96%, exhibited a particle size of 230 nm, zeta potential of −14.4 and poly disparity index of 0.261. Treatment with it led to cell morphology and DNA fragmentation as a characteristic of apoptosis. Flow cytometry showed G1 phase arrest of cell cycle and 26% increased apoptotic cells population upon treatment as compared to control. Sorafenib‐loaded casein nanoparticles showed 6‐fold increased ROS production in HepG2 cells as compared to 4‐fold increase shown by the free drug. Gene and protein expression studies done by qPCR and western blotting depicted upregulation of tumor suppressor gene p53, pro‐apoptotic Bax, and caspase‐3 along with downregulated anti‐apoptotic Bcl‐2 gene and protein expression which further emphasized death by apoptosis. It is concluded regarding the feasibility of these casein nanoparticles as a delivery system with enhanced therapeutic outcomes against hepatocellular carcinoma cells.

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Section: Cell Viability Assaymentioning

confidence: 99%

Evaluation of the cytotoxic activity of sorafenib‐loaded camel milk casein nanoparticles against hepatocarcinoma cells

Mittal,

Mahala,

Dhanawade

et al. 2024

Biotechnology Journal

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“…Moreover, the study carried out by Professor Cornish’s group has provided the first evidence that bLf exerts combined anabolic effects on osteoblasts and inhibitory effects on osteoporosis [ 13 ]. The current large-scale production techniques based on cation-exchange resins, followed by gel filtration, have been developed to isolate bLf from whey [ 14 ] and enable its use in products for the promotion of human health. However, the bioavailability of orally administered bLf is extremely low due to biochemical and physical barriers, including gastrointestinal proteases, the epithelial barrier of the small intestine, and efflux pumps [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Cellular Uptake and Transport of Bovine Lactoferrin Using Pectin- and Chitosan-Modified Solid Lipid Nanoparticles

Yao,

Bunt,

Liu

et al. 2023

Pharmaceutics

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Aim: The aim of this project is to use pectin- and chitosan-modified solid lipid nanoparticles for bovine lactoferrin to enhance its cellular uptake and transport. Methods: Solid lipid particles containing bovine lactoferrin (bLf) were formulated through the solvent evaporation technique, incorporating stearic acid along with either chitosan or pectin modification. bLf cellular uptake and transport were evaluated in vitro using the human adenocarcinoma cell line Caco-2 cell model. Results and Discussion: The bLf-loaded SLPs showed no significant effect on cytotoxicity and did not induce apoptosis within the eight-hour investigation. The use of confocal laser scanning microscopy confirmed that bLf follows the receptor-mediated endocytosis, whereas the primary mechanism for the cellular uptake of SLPs was endocytosis. The bLf-loaded SLPs had significantly more cellular uptake compared to bLf alone, and it was observed that this impact varied based on the time, temperature, and concentration. Verapamil and EDTA were determined to raise the apparent permeability coefficients (App) of bLf and bLf-loaded SLPs. Conclusion: This occurred because they hindered efflux by interacting with P-glycoproteins and had a penetration-enhancing influence. These findings propose the possibility of an additional absorption mechanism for SLPs, potentially involving active transportation facilitated by the P-glycoprotein transporter in Caco-2 cells. These results suggest that SLPs have the potential to be applied as effective carriers to improve the oral bioavailability of proteins and peptides.

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Assessment of Broad-Spectrum Antimicrobial, Antibiofilm, and Anticancer Potential of Lactoferrin Extracted from Camel Milk

Alkhulaifi,

Alosaimi,

Khan

et al. 2023

Appl Biochem Biotechnol

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Isolation and characterization of bioactive lactoferrin from camel milk by novel pH-dependent method for large scale production

Cited by 8 publications

References 38 publications

Evaluation of the cytotoxic activity of sorafenib‐loaded camel milk casein nanoparticles against hepatocarcinoma cells

Evaluation of the cytotoxic activity of sorafenib‐loaded camel milk casein nanoparticles against hepatocarcinoma cells

Enhanced Cellular Uptake and Transport of Bovine Lactoferrin Using Pectin- and Chitosan-Modified Solid Lipid Nanoparticles

Assessment of Broad-Spectrum Antimicrobial, Antibiofilm, and Anticancer Potential of Lactoferrin Extracted from Camel Milk

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