Isolation and Characterization of Bacteria That Grow on Methane and Organic Compounds as Sole Sources of Carbon and Energy

Patt, T. E.; Cole, G. C.; Bland, Judith A.; Hanson, Richard S.

doi:10.1128/jb.120.2.955-964.1974

Cited by 141 publications

(68 citation statements)

References 29 publications

(29 reference statements)

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“…Rows of the matrix are clustered using complete hierarchical clustering. (Patt et al, 1974;1976;Van Aken et al, 2004), but could never be independently verified (Dedysh et al, 2004a). Furthermore, its sole use of a sMMO and its marine origin as Alphaproteobacterial methanotroph makes strain R-67174 a unique discovery.…”

Section: Discussionmentioning

confidence: 99%

New Methyloceanibacter diversity from North Sea sediments includes methanotroph containing solely the soluble methane monooxygenase

Vekeman

Kerckhof

Cremers

et al. 2016

Environmental Microbiology

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SummaryMarine methylotrophs play a key role in the global carbon cycle by metabolizing reduced one-carbon compounds that are found in high concentrations in marine environments. Genome, physiology and diversity studies have been greatly facilitated by the numerous model organisms brought into culture. However, the availability of marine representatives remains poor. Here, we report the isolation of four novel species from North Sea sediment enrichments closely related to the Alphaproteobacterium Methyloceanibacter caenitepidi. Each of the newly isolated Methyloceanibacter species exhibited a clear genome sequence divergence which was reflected in physiological differences. Notably one strain R-67174 was capable of oxidizing methane as sole source of carbon and energy using solely a soluble methane monooxygenase and represents the first marine Alphaproteobacterial methanotroph brought into culture. Differences in maximum cell density of >1.5 orders of magnitude were observed. Furthermore, three strains were capable of producing nitrous oxide from nitrate. Together, these findings highlight the metabolic and physiologic variability within closely related Methyloceanibacter species and provide a new understanding of the physiological basis of marine methylotrophy.

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Section: Discussionmentioning

confidence: 99%

New Methyloceanibacter diversity from North Sea sediments includes methanotroph containing solely the soluble methane monooxygenase

Vekeman

Kerckhof

Cremers

et al. 2016

Environmental Microbiology

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“…Shortly thereafter, the first facultative methanotrophic isolates from freshwater lake sediments and water were reported. These could utilize a wide range of multicarbon compounds as growth substrates, including many organic acids (malate, succinate, fumarate, and acetate) and sugars (glucose, galactose, sucrose, lactose, and ribose) (Patt et al, 1974). One strain, later described as Methylobacterium organophilum (belonging to the Alphaproteobacteria), was further characterized, and had the complete tricarboxylic acid (TCA) cycle (Patt et al, 1976).…”

Section: Initial Findings Of Facultative Methanotrophymentioning

confidence: 99%

Facultative methanotrophy: false leads, true results, and suggestions for future research

Semrau

DiSpirito

Vuilleumier

2011

FEMS Microbiol Lett

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Methanotrophs are a group of phylogenetically diverse microorganisms characterized by their ability to utilize methane as their sole source of carbon and energy. Early studies suggested that growth on methane could be stimulated with the addition of some small organic acids, but initial efforts to find facultative methanotrophs, i.e., methanotrophs able to utilize compounds with carbon-carbon bonds as sole growth substrates were inconclusive. Recently, however, facultative methanotrophs in the genera Methylocella, Methylocapsa, and Methylocystis have been reported that can grow on acetate, as well as on larger organic acids or ethanol for some species. All identified facultative methanotrophs group within the Alphaproteobacteria and utilize the serine cycle for carbon assimilation from formaldehyde. It is possible that facultative methanotrophs are able to convert acetate into intermediates of the serine cycle (e.g. malate and glyoxylate), because a variety of acetate assimilation pathways convert acetate into these compounds (e.g. the glyoxylate shunt of the tricarboxylic acid cycle, the ethylmalonyl-CoA pathway, the citramalate cycle, and the methylaspartate cycle). In this review, we summarize the history of facultative methanotrophy, describe scenarios for the basis of facultative methanotrophy, and pose several topics for future research in this area.

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“…Mb. organophilum strains XX and RG, are facultative type II methanotrophs, and were grown in AMS basal salts medium [21] with an atmosphere of methane and air (1:4, v:v) or with methanol (0.1%, w:v) in the absence of methane.…”

Section: Bacteria and Culture Conditionsmentioning

confidence: 99%

Phospholipid and lipopolysaccharide normal and hydroxy fatty acids as potential signatures for methane-oxidizing bacteria

Nichols¹,

A²,

Antworth³

et al. 1985

FEMS Microbiology Letters

131

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The extractable ester‐linked and the lipopolysaccharide (LPS) normal and hydroxy fatty acids of the methylotrophic bacteria Methylosinus trichosporium 0B3B, Methylobacterium organophilum XX, grown on methane and methanol, Mb. organophilum RG and Methylomonas sp. were analysed by capillary gas chromotography‐mass spectrometry (GC‐MS). Precise monounsaturated double bond position and geometry was determined by GC‐MS analysis of the derivatized fatty acids. The three species were readily distinguished based on the extractable fatty acid and LPS hydroxy acid profiles. Type I and Type II methylotrophs can be separated based on the presence of 16‐carbon and 18‐carbon monoenoic fatty acids in the two groups of organisms, respectively. Relatively novel components, 18: 1ω8c, 18: 1ω8t, 18: 1ω7t and 18: 1ω6c were present in Ms. trichosporium, and 16: 1ω8c, 16: 1ω8t, 16: 1ω7t, 16: 1ω5c and 16: 1ω5t were detected in Methylomonas sp. These specific lipids may be used, together with other components, as signatures for these methylotrophic bacteria in manipulated laboratory and environmental samples.

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Isolation and Characterization of Bacteria That Grow on Methane and Organic Compounds as Sole Sources of Carbon and Energy

Cited by 141 publications

References 29 publications

New Methyloceanibacter diversity from North Sea sediments includes methanotroph containing solely the soluble methane monooxygenase

New Methyloceanibacter diversity from North Sea sediments includes methanotroph containing solely the soluble methane monooxygenase

Facultative methanotrophy: false leads, true results, and suggestions for future research

Phospholipid and lipopolysaccharide normal and hydroxy fatty acids as potential signatures for methane-oxidizing bacteria

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