Isolation and Characterization of Bacteria and Fungi Associated With Biodegradation of Municipal Solid Wastes in Abakaliki Metropolis, Nigeria

Emmanuel, Ugwu Chidiebere; Ifeanyichukwu, Iroha; Chika, Ejikeugwu; Chike, Onochie; Chinyere, Nwachi

doi:10.22161/ijeab/2.3.35

Cited by 4 publications

(5 citation statements)

References 8 publications

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“…Moisture Content (%) and pH Of Waste (Soil) Samples were carried out by applying the formula given by AWPA (American Wood Protection Association) (Emmanuel et al, 2017), whereas the pH was optimized via pH strips.…”

Section: Physicochemical Properties Of Solid Wastementioning

confidence: 99%

Molecular and biochemical characterization of solid waste biodegrading microbes isolated from municipal waste dump site

Afshan,

Ghouri,

Hayat

et al. 2023

Acta Bras.

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Solid waste dump site-based pollution is one of the colossal health concerns globally due to presence of cellulose, starch, protein, and lipids, which is a perfect nutrient waste for the development of biodegrading potential microbes. The current study aimed to determine the productive microorganisms for waste biodegradation. Ten samples were collected from different areas of Karachi waste dump sites. The identification and characterization of isolates were done by morphological and biochemical tests. The isolated microbes were qualitatively screened for the action of making industrially key bio-enzymes. The potential enzymes producing microbes were subjected to waste degradation test for 90 days. Changes of odor, color and weight loss of decomposing garbage were noted. The highest degradation ability in term of weight loss was shown by Aspergillus (SSI14) and Bacillus (SSI6). Four metals (zinc sulphate, cadmium chloride, copper sulphate and iron sulphate) were chosen for the purpose of metals tolerance test and minimum inhibitory concentration (MIC) of the isolated microbial strains. Current study reported that Zn was found to be more lethal in in comparison to Cu and Fe. Molecular identification of the isolated microbes was done by polymerase chain reaction. The microbial strains were characterized by the polymerase chain reaction (PCR) followed by confirmation of product by agarose gel electrophoresis. The findings can be served as baseline data to develop microbial apparatus for biodegradation and management of solid waste.

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Section: Physicochemical Properties Of Solid Wastementioning

confidence: 99%

Molecular and biochemical characterization of solid waste biodegrading microbes isolated from municipal waste dump site

Afshan,

Ghouri,

Hayat

et al. 2023

Acta Bras.

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“…A sterile spatula was used to scoop the subsurface at the depth of 10 cm. About 10 grams of solid waste sample was transferred to a sterile plastic container [11].…”

Section: Sample Collection and Transportationmentioning

confidence: 99%

“…Serial dilution was performed up to 10 -6 dilutions. Then, 0.1 ml sample, from 10 -2 , 10 -4 , and 10 -6 dilutions, was inoculated into different respective culture media plates (PCA, XLD, MA, CA, MSA, and SDA) [11].…”

Section: Sample Processingmentioning

confidence: 99%

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Microbial Assessment of Solid Waste and Bioaerosol Associated with Open Dumping Sites of the Kathmandu City, Nepal

Kapali,

Shakya,

Pradhan

et al. 2023

IJEAB

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The study aims to isolate and identify bacteria and fungi (mold) present in solid waste and its associated bioaerosol in the Kathmandu city. A total of 10 samples; 5 different solid waste samples and 5 different bioaerosol samples, collected from 5 open dumping sites in the Kathmandu city, were transported to the microbiology laboratory of St. Xavier’s College for processing. Standard microbiological procedures were followed for the identification of isolates. The Kirby-Bauer disk diffusion method was used to determine the antibiotic susceptibility of bacterial isolates following CLSI 2020 standards. In the collected solid waste samples, the bacterial colony count ranged from 1.27 × 108 to 2.8 × 108 CFU/ml, whereas the fungi colony count ranged from 1 × 105 to 4 × 105 CFU/ml. Bacterial colony counts from bioaerosol samples ranged from 116 to >300 CFU/90mm/15 minutes, whereas fungi colony counts were between 2 and 6 CFU/90mm/15 minutes. Out of 48 bacteria and 34 molds identified, Bacillus spp. (27%) and Aspergillus niger (29%) were found to be predominant than other isolates. Citrobacter spp., Salmonella spp., and Escherichia coli isolated from solid waste samples of dump site S3 showed maximum resistance to the different antibiotics used. The common microbial isolates from solid waste samples and bioaerosol samples included 7 different bacteria and 4 different molds. The presence of antibiotic-resistant bacteria and pathogenic fungi in waste dump sites pose public health-related risks.

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“…suggest that they possess higher abilities to breakdown and utilize the solid wastes. Previous studies have reported that the bacterial isolates obtained from the dumpsites were efficient degraders and utilizers of organic waste components, and they were used as sole sources of carbon and energy (Emmanuel et al, 2017).…”

Section: Isolation and Identification Of The Bacteriamentioning

confidence: 99%

Biodegradation of plastic materials obtained from solid waste dumpsites in Nigeria, using native bacterial strains

Ojiego

Ilo

Dantanko

et al. 2022

Novel Research in Microbiology Journal

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Plastic packaging materials constitute a major potential environmental pollutant due to their slow degradation rates. This study aimed to isolate the plastic-degrading bacteria from the solid waste dumpsites of Abuja, Nigeria. Soil samples (n= 72) and plastic materials (bottles and bags) were collected from the dumpsites using soil augers and manual picking, respectively. Bacteriological analysis of the soil samples revealed the recovery of a total of 54 bacterial isolates, which were distributed among the genera of; Proteus sp. ( 33.3 %), Providencia sp. (29.63 %), Pseudomonas sp. (16.67 %), Bacillus sp. (9.26 %), Micrococcus sp. (5.56 %), Escherichia coli (1.85 %), Enterobacter sp. (1.85 %), and Serratia sp. (1.85 %).The bacterial isolates were inoculated into a series of shake flasks containing nutrient broth and pre-sterilized strips (1×1 cm) of plastic bags (0.05-0.0514 g) and plastic bottles (0.05-0.0529 g), and then incubated at 30 o C for 60 d to monitor their biodegradation using the weight loss method. The strips of bottles (0.58-49.00 %) were more susceptible to biodegradation than the plastic bags (0.78-15.40 %) after 60 d of incubation. The results demonstrated that about 6 of the bacterial isolates belong to the two genera of Proteus sp. and Providencia spp., and were considered the best bio-degraders. Molecular characterization of these potent isolates has identified them as Proteus mirabilis strain PPB3 (49.00 %), Proteus mirabilis strain UPMSD3 (32.07 %), Proteus mirabilis strain HH133 (20.41 %), Proteus mirabilis strain SSBIKEN (15.40 %), Providencia vermicola strain M4 (14.96 %), and Providencia vermicola strain 11 (12.20 %). These strains could be considered as potential biodegradation agents for the plastic materials that are prevalent in dumpsites.

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Isolation and Characterization of Bacteria and Fungi Associated With Biodegradation of Municipal Solid Wastes in Abakaliki Metropolis, Nigeria

Abstract: Abstract-Microorganisms involved in the biodegradation of municipal solid waste (MSW) in Abakaliki dumps

Cited by 4 publications

References 8 publications

Molecular and biochemical characterization of solid waste biodegrading microbes isolated from municipal waste dump site

Molecular and biochemical characterization of solid waste biodegrading microbes isolated from municipal waste dump site

Microbial Assessment of Solid Waste and Bioaerosol Associated with Open Dumping Sites of the Kathmandu City, Nepal

Biodegradation of plastic materials obtained from solid waste dumpsites in Nigeria, using native bacterial strains

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