Isolation and Characterization of Bacteria for Amylase Production under Solid State Fermentation Using Damaged Wheat as Substrate

Razdan, Nadia; Kocher, GS

doi:10.20546/ijcmas.2018.707.114

Cited by 2 publications

(2 citation statements)

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“…Amylase activity of the amylolytic fungi on different organic substrates viz; 100% corn (C); rice (R); wheat (W); millet (M); 1:1 corn and rice (CR); corn and wheat (CW); corn and millet (CM); rice and wheat (RW); rice and millet (RM); wheat and millet (WM); 1:2:1 corn, rice and wheat (CRW); rice, corn and millet (RCM); millet, wheat and rice (MWR); wheat, millet and corn (WMC); 1:1:1:1 corn, rice, wheat and millet (CRWM). In SSF, the selection of a suitable culture medium and a suitable microbial strain are key factors for the production of α-amylases (Razdan and Kocher, 2018). This study revealed appreciable amylase production by A. flavus in the composition of rice and millet.…”

Section: Resultsmentioning

confidence: 78%

“…Also, Aspergillus niger highly opposes contamination and is acid-tolerant (Gurung et al, 2013). Solid substrate fermentation (SSF) has been considered a promising technique for the production of enzymes due to its economic and engineering advantages (Razdan and Kocher, 2018). In recent years, the SSF process has been developed and extensively used for the synthesis of bio-products, especially when it involves fungi (Kwatia and Dzogbefia, 2018).…”

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confidence: 99%

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Catalytic properties of purified alpha amylase from Aspergillus flavus cultivated on low-cost agricultural substrate

Owolabi,

Olaniyi,

Akinyosoye

2023

Rev. Fac. Nac. Agron. Medellín

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Aspergillus flavus isolated from fermented millet flour produced a crude enzyme, which was purified via ammonium sulphate precipitation and subsequent chromatographic techniques. The biochemical characteristics of the purified amylase were thereafter investigated showing activity in a wide range of pH and temperature, with optimal conditions of pH 6.0 and 50 °C. The enzyme retained even 89% of its activity after 1 h at 50 °C and 2 h at pH 6.0. The purified enzyme was stimulated by Ca2+, Zn2+ and Co2+, while Hg2+ and EDTA caused mild inhibition of α-amylase activity. The kinetic indices (Km and Vmax) and molecular weight of the enzyme were estimated in 1.71 mg mL-1, 2.133 μmol min-1 mL-1 and 45 kDa respectively. The catalytic properties of α-amylase from A. flavus makes it a promising candidate for use in various starch processing industries.

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Section: Resultsmentioning

confidence: 78%

mentioning

confidence: 99%

Catalytic properties of purified alpha amylase from Aspergillus flavus cultivated on low-cost agricultural substrate

Owolabi,

Olaniyi,

Akinyosoye

2023

Rev. Fac. Nac. Agron. Medellín

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Isolation, Screening and Antimicrobial Activity of Aeromonas hydrophila from Spoiled Vegetables and Biochemical Analysis of Estuarine Microbial Sediments

Kumar

Duraisamy

Murali

et al. 2020

CBIOT

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Background: The Aeromonas hydrophila is a heterotrophic, Gram-negative, rod-shaped, facultative anaerobic, nonspore forming bacteria that are autochthonous and widely dispersed in marine environments. In this work design to investigate the screening of Aeromonas hydrophila from spoiled vegetable and three different estuarine sediments sample were collected from different estuaries of Bay of Bengal (Vedharanyam, Parangipettai and Pichavaram, Tamilnadu, India) for enzymes and antimicrobial activities. Objective: Isolation, enzyme screening, antimicrobial activity of Aeromonas hydrophila from spoiled vegetables and three different estuarine microbial sediments sample were used to analysis the biochemical and enzymatic analysis. Results: The purified isolate of Aeromonas hydrophila strain produces good antimicrobial activity against Aspergillus niger, Candida albicans, Staphylococus, Klebsiella and pseudomonas species. Methods: The bioactive compound produced by this strain was purified by thin layer chromatography. Conclusion: These isolates producing amylase, protease, lipase and gelatinase enzymes, which are commercially very important and used in many industries and other biochemical sectors.

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Isolation and Characterization of Bacteria for Amylase Production under Solid State Fermentation Using Damaged Wheat as Substrate

Cited by 2 publications

References 10 publications

Catalytic properties of purified alpha amylase from Aspergillus flavus cultivated on low-cost agricultural substrate

Catalytic properties of purified alpha amylase from Aspergillus flavus cultivated on low-cost agricultural substrate

Isolation, Screening and Antimicrobial Activity of Aeromonas hydrophila from Spoiled Vegetables and Biochemical Analysis of Estuarine Microbial Sediments

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