Isolation and characterization of Bacillus endophytic strains producers for non ribosomal lipopeptides NRLPs from tomato

Abstract

Tomato (Solanum lycopersicum) are consid­ered one of the most important vegetable crops and infected by numbers of different diseases. Studying the use of biological alternatives, instead of chemical substances against plant diseases became necessary for the treatment by beneficial microorganisms endophytes, which can excrete natural products benefits to plant in reducing disease severity, promoting growth and inducing plant defence mechanisms. In this work, three endophytes strains were isolated from tomato stems and their 16srDNA have been found to belong to Bacillus species. The first strain was named BMG100, the second BMG101 and the third BMG102. Two Bacillus strains BMG100 and BMG101 have been found to harbour synthetases genes from three lipopeptides families; surfactin, plipastatin, and iturin (mycosubtilin) which can be detected by degenerated primers designed to detect the presence of synthetases genes encoding lipopeptides. The lipopeptides production was proved by their quantification using High Performance Liquid Chromatography (HPLC), whereas BMG100 produced 105, 178 and 293 mg/L of plipastatin, mycosubtilin and surfactin, respectively, BMG101 produced 385 mg/L of surfactin and 236 mg/L of mycosubtilin, while BMG102 showed no lipopeptides production.
 Keywords: Tomato; Endophytic bacteria; Lipopeptides; Bacillus species

Regulation, Biosynthesis, and Extraction of Bacillus-Derived Lipopeptides and Its Implications in Biological Control of Phytopathogens

“…For instance, PCR analysis is directed to identify the presence or absence of genes (gene-level) involved in the biosynthesis and assembly of lipopeptides [117], whereas analytical tools such as liquid chromatography or mass spectrometry are focused on identifying the lipopeptide itself (metabolite-level) [118]. Each method varies based on the purpose and maturity stage of the study.…”

“…Current knowledge about the biosynthesis, structure, and bioactivity of Bacillus lipopeptides has allowed the development of a great variety of methods to identify lipopeptide-producer strains across different information levels (i.e., phenotype, DNA, RNA, protein, and metabolites) (Figure 7). For instance, PCR analysis is directed to identify the presence or absence of genes (gene-level) involved in the biosynthesis and assembly of lipopeptides [117], whereas analytical tools such as liquid chromatography or mass spectrometry are focused on identifying the lipopeptide itself (metabolite-level) [118]. Each method varies based on the purpose and maturity stage of the study.…”