Isolation and characterization of avian metapneumovirus from chickens in Korea

Kwon, Jun-Hun; Lee, Hyun-Jeong; Jeong, Seung-Hwan; Park, Jeong-Yong; Hong, Young-Ho; Lee, Youn‐Jeong; Youn, Ho-Sik; Lee, Dong Hoon; Do, Sun-Hee; Park, Seung‐Yong; Choi, In‐Soo; Lee, Joong‐Bok; Song, Chang‐Seon

doi:10.4142/jvs.2010.11.1.59

Cited by 27 publications

(28 citation statements)

References 27 publications

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“…Kwon et al reported that two aMPV-As out of the 25 detected aMPV were successfully isolated in Vero cells [29]. Our study had similar results.…”

Section: Discussionsupporting

confidence: 88%

“…The pathogen was serially cultured through 13 passages in Vero cells and then identified by electron microscopy. We observed that the pathogen particle was an enveloped virus characterized by a diameter of 20-500 nm and multiple morphologies ( Figure 3E, F), similar to that of aMPV [14,29]. Vero cells were inoculated with S-01 at an MOI of 0.…”

Section: Novel Virus Is Isolated and Characterized As Ampv-like Virusmentioning

confidence: 88%

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Isolation and characterization of a subtype C avian metapneumovirus circulating in Muscovy ducks in China

Sun

Chen

Cao

et al. 2014

Vet Res

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Subtype C avian metapneumovirus (aMPV-C), is an important pathogen that can cause egg-drop and acute respiratory diseases in poultry. To date, aMPV-C infection has not been documented in Muscovy ducks in China. Here, we isolated and characterized an aMPV-C, designated S-01, which has caused severe respiratory disease and noticeable egg drop in Muscovy duck flocks in south China since 2010. Electron microscopy showed that the isolate was an enveloped virus exhibiting multiple morphologies with a diameter of 20-500 nm. The S-01 strain was able to produce a typical cytopathic effect (CPE) on Vero cells and cause death in 10-to 11-day-old Muscovy duck embryos. In vivo infection of layer Muscovy ducks with the isolate resulted in typical clinical signs and pathological lesions similar to those seen in the original infected cases. We report the first complete genomic sequence of aMPV-C from Muscovy ducks. A phylogenetic analysis strongly suggested that the S-01 virus belongs to the aMPV-C family, sharing 92.3%-94.3% of nucleotide identity with that of aMPV-C, and was most closely related to the aMPV-C strains isolated from Muscovy ducks in France. The deduced eight main proteins (N, P, M, F, M2, SH, G and L) of the novel isolate shared higher identity with hMPV than with other aMPV (subtypes A, B and D). S-01 could bind a monoclonal antibody against the F protein of hMPV. Together, our results indicate that subtype-C aMPV has been circulating in Muscovy duck flocks in South China, and it is urgent for companies to develop new vaccines to control the spread of the virus in China.

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“…Kwon et al reported that two aMPV-As out of the 25 detected aMPV were successfully isolated in Vero cells [29]. Our study had similar results.…”

Section: Discussionsupporting

confidence: 88%

Section: Novel Virus Is Isolated and Characterized As Ampv-like Virusmentioning

confidence: 88%

Isolation and characterization of a subtype C avian metapneumovirus circulating in Muscovy ducks in China

Sun

Chen

Cao

et al. 2014

Vet Res

View full text Add to dashboard Cite

show abstract

“…The pathogen was serially cultured through 13 passages in Vero cells and then identified by electron microscopy. We observed that the pathogen particle was an enveloped virus characterized by a diameter of 20–500 nm and multiple morphologies (Figure 3E, F), similar to that of aMPV [14],[29]. Vero cells were inoculated with S-01 at an MOI of 0.1.…”

Section: Resultsmentioning

confidence: 99%

“…The virion particles in the pellet were visualized by electron microscopy (EM) as described by Kwon et al [29]. …”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of a subtype C avian metapneumovirus circulating in Muscovy ducks in China

Sun

Chen

Cao

et al. 2014

Vet Res

View full text Add to dashboard Cite

Subtype C avian metapneumovirus (aMPV-C), is an important pathogen that can cause egg-drop and acute respiratory diseases in poultry. To date, aMPV-C infection has not been documented in Muscovy ducks in China. Here, we isolated and characterized an aMPV-C, designated S-01, which has caused severe respiratory disease and noticeable egg drop in Muscovy duck flocks in south China since 2010. Electron microscopy showed that the isolate was an enveloped virus exhibiting multiple morphologies with a diameter of 20–500 nm. The S-01 strain was able to produce a typical cytopathic effect (CPE) on Vero cells and cause death in 10- to 11-day-old Muscovy duck embryos. In vivo infection of layer Muscovy ducks with the isolate resulted in typical clinical signs and pathological lesions similar to those seen in the original infected cases. We report the first complete genomic sequence of aMPV-C from Muscovy ducks. A phylogenetic analysis strongly suggested that the S-01 virus belongs to the aMPV-C family, sharing 92.3%-94.3% of nucleotide identity with that of aMPV-C, and was most closely related to the aMPV-C strains isolated from Muscovy ducks in France. The deduced eight main proteins (N, P, M, F, M2, SH, G and L) of the novel isolate shared higher identity with hMPV than with other aMPV (subtypes A, B and D). S-01 could bind a monoclonal antibody against the F protein of hMPV. Together, our results indicate that subtype-C aMPV has been circulating in Muscovy duck flocks in South China, and it is urgent for companies to develop new vaccines to control the spread of the virus in China.

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“…It causes acute rhinotracheitis (TRT) in turkeys and swollen head syndrome (SHS) in chickens, leading to serious financial losses [18][19][20]. Since aMPV was first detected in South Africa in 1978 [21], many countries and regions have reported aMPV-infected poultry [22][23][24][25][26][27][28][29]. Based on the classification of hMPV, aMPV strains have been divided into four subtypes, A, B, C, and D (also designated aMPV/A, aMPV/B, aMPV/C, and aMPV/D) [22,[30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Effect of amino acid sequence variations at position 149 on the fusogenic activity of the subtype B avian metapneumovirus fusion protein

et al. 2015

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The entry of enveloped viruses into host cells requires the fusion of viral and cell membranes. These membrane fusion reactions are mediated by virus-encoded glycoproteins. In the case of avian metapneumovirus (aMPV), the fusion (F) protein alone can mediate virus entry and induce syncytium formation in vitro. To investigate the fusogenic activity of the aMPV F protein, we compared the fusogenic activities of three subtypes of aMPV F proteins using a TCSD50 assay developed in this study. Interestingly, we found that the F protein of aMPV subtype B (aMPV/B) strain VCO3/60616 (aMPV/vB) was hyperfusogenic when compared with F proteins of aMPV/B strain aMPV/f (aMPV/fB), aMPV subtype A (aMPV/A), and aMPV subtype C (aMPV/C). We then further demonstrated that the amino acid (aa) residue 149F contributed to the hyperfusogenic activity of the aMPV/vB F protein. Moreover, we revealed that residue 149F had no effect on the fusogenic activities of aMPV/A, aMPV/C, and human metapneumovirus (hMPV) F proteins. Collectively, we provide the first evidence that the amino acid at position 149 affects the fusogenic activity of the aMPV/B F protein, and our findings will provide new insights into the fusogenic mechanism of this protein.

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Isolation and characterization of avian metapneumovirus from chickens in Korea

Cited by 27 publications

References 27 publications

Isolation and characterization of a subtype C avian metapneumovirus circulating in Muscovy ducks in China

Isolation and characterization of a subtype C avian metapneumovirus circulating in Muscovy ducks in China

Isolation and characterization of a subtype C avian metapneumovirus circulating in Muscovy ducks in China

Effect of amino acid sequence variations at position 149 on the fusogenic activity of the subtype B avian metapneumovirus fusion protein

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