Isolation and characterization of apolipophorin-III from the giant water bug (Lethocerus medius)

Cloning and expression of apolipophorin-III from the common cutworm,Spodoptera litura

“…This prediction was supported by our measurements of circular dichroism. The CD spectrum indicated a considerable content of α-helix (61.5%) and this result is in agreement with the previously reported data from apoLp-III of other insects (Kawooya et al, 1986;Kanost et al, 1995). A helical wheel projection of S. litura apoLp-III demonstrated spatial segregation of its hydrophobic and charged residues, indicating the amphipathic nature of α-helix of this particle.…”

Apolipophorin III: a lipid-triggered molecular switch

“…Besides this, apoLp-III has been isolated and studied from various Lepidopteran species, including Bombyx mori, Bombyx mandarina, Spodoptera litura, G. mellonella, Acherontia atropos, Diatraea grandiosella, and Heliothis virescens (Burks et al, 1992;Surholt et al, 1992;Smith et al, 1994;Kim et al, 1998;Weise et al, 1998;Yamauchi et al, 2000;Chung and Ourth, 2002). Orthoptera species include Gastrimargus africanus, Acheta domesticus, Barytettix psolus, and Melanoplus differentialis (Haunerland et al, 1986;Ryan et al, 1990), Coleoptera: Derobrachus geminatus (Smith et al, 1994) and Hemiptera: Lethocerus medius (Kanost et al, 1995). Lipid-free apoLp-III is highly soluble in buffer (Ͼ30 mg/ml) and is monomeric even at these high protein concentrations.…”

Purification and characterization of apolipophorin‐III in the hemolymph of the wax moth,galleria MellonellaL.

“…Molecular mass of apoLp-III was reported to be 20 kDa in Barytettix psolus (Ziegler et al, 1988), Locusta migratoria (Chino and Yazawa, 1986), Gastimargus africanus (Haunerland et al, 1986), 19 kDa in Lethocerus medius (Kanost et al, 1995), 18 kDa in Hyphantria cunea , and 17 kDa in Manduca sexta (Shapiro and Law, 1983). However, the two molecular species of apoLp-lll were not described so far.…”

“…ApoLp-lll has been studied in Locusta migratoria, Gastimargus africanus, Thasus acutangulus, Lethocerus medius, and Hyphantria cunea (Kawooya et al, 1984;Wells et al, 1985;Chino and Yazawa, 1986;Haunerland et al, 1986;Kanost et al, 1995; since purification and characteristics of apoLp-lll in the adult hemolymph of Manduca sexta has been reported by Shapiro and Law (1983). Molecular mass of apoLp-III was reported to be 20 kDa in Barytettix psolus (Ziegler et al, 1988), Locusta migratoria (Chino and Yazawa, 1986), Gastimargus africanus (Haunerland et al, 1986), 19 kDa in Lethocerus medius (Kanost et al, 1995), 18 kDa in Hyphantria cunea , and 17 kDa in Manduca sexta (Shapiro and Law, 1983).…”