Isolation and characterization of antimicrobial peptides derived from Bacillus subtilis E20-fermented soybean meal and its use for preventing Vibrio infection in shrimp aquaculture

Cheng, An; Lin, Hsueh‐Li; Shiu, Ya‐Li; Tyan, Yu‐Chang; Liu, Chun-Hung

doi:10.1016/j.fsi.2017.06.006

Cited by 94 publications

(51 citation statements)

References 41 publications

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“…Antimicrobial peptide has a large spectrum, kills more pathogens and also has low resistance potential as compared with antibiotics (Kim et al, ). Several studies have been done on the use of AMP in shrimp and fish, and it improves innate immune response and antioxidant function (Cheng, Lin, Shiu, Tyan, & Liu, ; Ting, Chen, & Chen, ).…”

Section: Introductionmentioning

confidence: 99%

Effects of antimicrobial peptides on growth, feed utilization, serum biochemical indices and disease resistance of juvenile shrimp,Litopenaeus vannamei

et al. 2020

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This experiment was conducted to assess the effects of antimicrobial peptides at different levels (Diet 1 (0%), Diet 2 (0.1%), Diet 3 (0.2%), Diet 4 (0.4%), Diet 5 (0.6%) and Diet 6 (1%)), on growth, serum biochemical indices and antioxidant effect, feed utilization and disease resistance in Litopeneaus vannamei. There were four replicates in each group in the experiment (mean weight = 0.21 ± 0.00 g) and also fed with their respective diets for 8 weeks. Growth performance compared with the control group (0%) significantly increased at first and then decreased among treatment groups (p < .05) whereas the survival rate ranged from 78% to 96%. No significant differences were observed in terms of moisture, crude protein and ash content, but there was a significant increase in crude lipid (p < .05). In serum, acid phosphatase, alkaline phosphatase, polyphenol oxidase, triglyceride, glucose and total cholesterol changed as compared with 0%. Total antioxidant capacity, glutathione peroxidase, catalase, superoxide dismutase and malondialdehyde were also different from 0%. Disease resistance was increased in shrimp among treatment groups with 0.4% recording the lowest mortality percentage of 37% after the challenge test. The results from the present study suggested that supplementation of AMP at 0.4% in shrimp diet can improve growth performance, antioxidants activities and innate immune response of Pacific whiteleg shrimp.

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Section: Introductionmentioning

confidence: 99%

Effects of antimicrobial peptides on growth, feed utilization, serum biochemical indices and disease resistance of juvenile shrimp,Litopenaeus vannamei

et al. 2020

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“…Several stratagems have been applied to ameliorate problems of shrimp aquaculture, such as the use of genetically resistant stock, and dietary supplements with immunostimulants, such as medicinal plant products, antimicrobial compounds and probiotics (Cheng, Lin, Shiu, Tyan, & Liu, ; Kumar, Roy, Meena, & Sarkar, ; Shiu, Lin, Chi, Yeh, & Liu, ; Yeh et al, ; Zorriehzahra et al, ). Among these, biocontrol with probiotics is considered to be more environmentally friendly, effective and practical for aquaculture farmers to improve the water quality, health status and growth of fish and shrimp (Kumar et al, ; Lin, Shiu, Chiu, Huang, & Liu, ; Wang, Hu, Chiu, & Liu, ).…”

Section: Introductionmentioning

confidence: 99%

The growth and apparent digestibility of white shrimp,Litopenaeus vannamei, are increased with the probiotic,Bacillus subtilis

2019

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The aims of this study were to evaluate the probiotic efficiency of Bacillus subtilis E20 (109 colony‐forming units kg−1) on the growth and apparent digestibility coefficients (ADCs) of dry matter (DM), crude protein (CP), amino acids (AAs), and crude lipids (CLs) of the white shrimp, Litopenaeus vannamei. A control diet without probiotic supplementation was used. Shrimp exhibited significant increases in growth performance and feed utilization after being fed the test diet for 56 days. Shrimp were then used to evaluate the apparent nutrient digestibility using 0.1% Cr2O3 in the diet as an indicator. During the trial, faeces was collected from triplicate groups of shrimp twice daily. ADCs of CP and DM in the test diet significantly increased compared to those of the control diet. Although the ADCs of CLs did not significantly differ between the control and probiotic group, the ADC of CLs was higher in the probiotic group than in the control group. In addition, ADCs of most AAs in the test diet were significantly higher compared to those of the control diet. These results suggest that shrimp in the test group had significantly better growth performance, which was due to increases in nutrient digestibility and absorption.

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“…In addition to the above‐mentioned benefits, probiotics are used to improve the nutritional value and utilization of plant ingredients through fermentation (Chiu et al, ; Van Nguyen, Hoang, Van Khanh, Duy Hai, & Hung, ; Shiu, Hsieh, Guei, Chiu, & Liu, ; Shiu, Wong, Guei, Shin, & Liu, ). Furthermore, some studies showed that fermented soybean meal (FSBM) contains biologically active peptides that exert physiological effects on microbes in the gut (Erdmann, Cheung, & Schröder, ), including antimicrobial activity (Kim, Kim, Ko, Lee, Ha, Song, Park, Kwon, Bae, ; Sanjukta & Rai, ; Cheng, Lin, Shiu, Tyan, & Liu, ). In our previous study, a biofunctional ingredient, Bacillus subtilis E20‐FSBM, was shown to have antimicrobial peptides (AMPs) that inhibited two important shrimp pathogens: Vibrio alginolyticus and V. parahemolyticus .…”

Section: Introductionmentioning

confidence: 99%

“…subtilis E20‐FSBM (FSBMD) and a diet containing an AMP isolated from B . subtilis E20‐FSBM (AMPD) had better disease resistance after an oral challenge with V. parahemolyticus (Cheng et al, ), suggesting that FSBMDs or AMPDs might alter the composition of microorganisms in the intestine of shrimp and reduce the risk of disease.…”

Section: Introductionmentioning

confidence: 99%

Intestinal microbiota of white shrimp, Litopenaeus vannamei, fed diets containing Bacillus subtilis E20‐fermented soybean meal (FSBM) or an antimicrobial peptide derived from B. subtilis E20‐FSBM

Cheng

Yeh

et al. 2019

Aquac Res

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The purpose of the present study is to profile the microfloral structure in the intestines of the white shrimp, Litopenaeus vannamei, after being fed a control diet, in comparison with the diets containing Bacillus subtilis E20‐fermented soybean meal (FSBM) or an antimicrobial peptide (AMP) isolated from B. subtilis E20‐FSBM (diets designated FSBMD and AMPD, respectively) for 60 days. Metagenomic data showed detection of eight phyla, 20 classes, 40 orders, 68 families and 96 genera. Despite no statistically significant difference, an evaluation of microbial diversity recorded higher species richness, Shannon–Weaver diversity index and evenness in the AMPD and FSBMD groups, compared to the control diet group. Venn diagrams showed that 58 of the operational taxonomic units (OTUs) were shared among the control, FSBMD and AMPD groups, but additional beneficial microbes were only found in the FSBMD and AMPD groups. In addition to the augmentation of beneficial bacteria in the FSBMD and AMPD groups, the abundance of potential pathogens, Vibrio and Flavobacterium, was lower in the gut of shrimp fed the FSBMD and AMPD. The results clearly suggest that the FSBMD and AMPD have the capability to change the microfloral structure of shrimp intestines and could be used for disease prevention in shrimp aquaculture.

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Isolation and characterization of antimicrobial peptides derived from Bacillus subtilis E20-fermented soybean meal and its use for preventing Vibrio infection in shrimp aquaculture

Cited by 94 publications

References 41 publications

Effects of antimicrobial peptides on growth, feed utilization, serum biochemical indices and disease resistance of juvenile shrimp,Litopenaeus vannamei

Effects of antimicrobial peptides on growth, feed utilization, serum biochemical indices and disease resistance of juvenile shrimp,Litopenaeus vannamei

The growth and apparent digestibility of white shrimp,Litopenaeus vannamei, are increased with the probiotic,Bacillus subtilis

Intestinal microbiota of white shrimp, Litopenaeus vannamei, fed diets containing Bacillus subtilis E20‐fermented soybean meal (FSBM) or an antimicrobial peptide derived from B. subtilis E20‐FSBM

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