Isolation and Characterization of Antifungal Substances from Burkholderia sp. Culture Broth

Mao, Sopheareth; Lee, Seung-Je; Hwangbo, Hoon; Kim, Yong-Woong; Park, Keun-Hyung; Cha, Gyu-Suk; Park, Ro-Dong; Kim, Kil-Yong

doi:10.1007/s00284-005-0333-2

Cited by 52 publications

(27 citation statements)

References 28 publications

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“…3C), suggesting that most of the activity against F. moniliforme is related to other, as yet unknown, metabolites. Burkholderia species are well known for their production of a range of antifungal metabolites, including phenylacetic acid, hydrocinnamic acid, 4-hydroxyphenylacetic acid, and 4-hydroxyphenylacetate methyl ester (39,57). The role of these compounds, if present in the isolated strains, in the control of fungal diseases and endophytic colonization of sugarcane remains to be investigated.…”

Section: Resultsmentioning

confidence: 99%

Diversity of Cultivated Endophytic Bacteria from Sugarcane: Genetic and Biochemical Characterization of Burkholderia cepacia Complex Isolates

Mendes

Pizzirani-Kleiner

Araújo

et al. 2007

Appl Environ Microbiol

211

114

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Bacteria were isolated from the rhizosphere and from inside the roots and stems of sugarcane plants grown in the field in Brazil. Endophytic bacteria were found in both the roots and the stems of sugarcane plants, with a significantly higher density in the roots. Many of the cultivated endophytic bacteria were shown to produce the plant growth hormone indoleacetic acid, and this trait was more frequently found among bacteria from the stem. 16S rRNA gene sequence analysis revealed that the selected isolates of the endophytic bacterial community of sugarcane belong to the genera of Burkholderia, Pantoea, Pseudomonas, and Microbacterium. Bacterial isolates belonging to the genus Burkholderia were the most predominant among the endophytic bacteria. Many of the Burkholderia isolates produced the antifungal metabolite pyrrolnitrin, and all were able to grow at 37°C. Phylogenetic analyses of the 16S rRNA gene and recA gene sequences indicated that the endophytic Burkholderia isolates from sugarcane are closely related to clinical isolates of the Burkholderia cepacia complex and clustered with B. cenocepacia (gv. III) isolates from cystic fibrosis patients. These results suggest that isolates of the B. cepacia complex are an integral part of the endophytic bacterial community of sugarcane in Brazil and reinforce the hypothesis that plant-associated environments may act as a niche for putative opportunistic human pathogenic bacteria.Brazil is one of the world's largest sugarcane producers and has considerable influence over the international sugar market. Brazilian sugarcane is primarily used to produce sugar and alcohol. Production has increased over time to approximately 26 million tons of sugar and 16 million m 3 of alcohol in 2006. Although ethanol has been used as an alternative source of fuel in Brazil since 1980, it is currently receiving worldwide interest as a biofuel to replace, at least in part, gasoline, thereby contributing to a reduction in carbon emissions (33). Consequently, sustaining and enhancing the growth and yield of sugarcane have become a major focus of research. The growth and performance of sugarcane in the field are adversely affected by a number of abiotic and biotic factors, including a wide range of fungal and bacterial diseases. Pokkah boeng, caused by the fungus Fusarium moniliforme, is one of the most widespread diseases and may cause serious yield losses in commercial sugarcane plantings (68). F. moniliforme can be disseminated horizontally by airborne spores or crop debris and vertically through seed pieces. Current control strategies involve the use of resistant varieties and fungicide applications. The efficacy of both control measures, however, is limited, and there is an increasing need for novel and environmentally sound strategies to control this and other diseases of sugarcane.The overall goal of this study was to isolate and characterize beneficial bacteria that are intimately associated with sugarcane and have the potential to control pathogens and to promote the growth and yie...

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Section: Resultsmentioning

confidence: 99%

Diversity of Cultivated Endophytic Bacteria from Sugarcane: Genetic and Biochemical Characterization of Burkholderia cepacia Complex Isolates

Mendes

Pizzirani-Kleiner

Araújo

et al. 2007

Appl Environ Microbiol

211

114

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“…1). A primary mechanism of pathogen inhibition used by plant growth promoting rhizobacteria (PGPR) includes the production of antibiotics, lytic enzymes, volatile compounds, and siderophores (Mao et al, 2006). Antibiotics and lytic enzymes in particular are well known antifungal compounds that can directly or indirectly protect plants from pathogenic attack.…”

Section: Discussionmentioning

confidence: 99%

Biocontrol Potential of Streptomyces griseus H7602 Against Root Rot Disease (Phytophthora capsici) in Pepper

Nguyen¹,

Naing²,

Lee³

et al. 2012

The Plant Pathology Journal

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The root rot of pepper (Capsicum annuum L.) caused by Phytophthora capsici is one of the most important diseases affecting this crop worldwide. This work presents the evaluation of the capacity of Streptomyces griseus H7602 to protect pepper plants against Phytophthora capsici and establishes its role as a biocontrol agent. In this study, we isolated an actinomycete strain H7602 from rhizosphere soil, identified it as Streptomyces griseus by 16S rRNA analysis and demonstrated its antifungal activity against various plant pathogens including P. capsici. H7602 produced lytic emzymes such as chitinase, β-1,3-glucanase, lipase and protease. In addition, crude extract from H7602 also exhibited destructive activity toward P. capsici hyphae. In the pot trial, results showed the protective effect of H7602 against pepper from P. capsici. Application of H7602 culture suspension reduced 47.35% of root mortality and enhanced growth of pepper plants for 56.37% in fresh root and 17.56% g in fresh shoot as compared to control, resulting in greater protection to pepper plants against P. capsici infestation. Additionally, the enzymatic activities, chitinase and β-1,3-glucanase, were higher in rhizosphere soil and roots of pepper plants treated with H7602 than other treated plants. Therefore, our results indicated a clear potential of S. griseus H7602 to be used for biocontrol of root rot disease caused by P. capsici in pepper.

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“…Increasing concentration has invers effect on fugal growth. A mechanism of pathogen inhibition used by antagonistic microorganism is through production of antibiotics, lytic enzymes, volatile compounds and siderophores (Mao et al, 2006). Bacterial cultures filtrate of 10% concentration showing significant antifungal activity as shown in figure 2.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Biocontrol Potential of Bacillus amyloliquefaciens Y1 against Fungal Plant Pathogens

Jamal¹,

Lee²,

Jeon³

et al. 2015

Korean Journal of Soil Science and Fertilizer

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This study was performed to investigate thermophilic bacteria from soil having broad antifungal spectrum against Rhizoctonia solani, Colletotrichum gloeosporioides, Phytophthora capsici, Fusarium oxysporum f.sp. lycopersici, and Botrytis cinerea. One isolate selected could resist heat shock of 60°C for one hour, and had broad antifungal activity in dual culture assay against all tested fungal pathogens and was identified as Bacillus amyloliquefaciens Y1 using 16S rRNA gene sequence. Further investigation for antifungal activity of bacterial culture filtrate (BCF) and butanol crude extract (BCE) of various concentrations showed broad spectrum antifungal activity and fungal growth inhibition significantly increased with increasing concentration with highest growth inhibition of 100% against R. solani with 50% BCF and 11 mm of zone of inhibition against R. solani with 4 mg BCE concentration. Treatment of butanol crude extract resulted in deformation, lysis or degradation of C. gloeosporioides and P. capsici hyphae. Furthermore, B. amyloliquefaciens Y1 produced volatile compounds inhibiting growth of R. solani (70%), C. gloeosporioides (65%) and P. capsici (65-70%) when tested in volatile assay. The results from the study suggest that B. amyloliquefaciens Y1 could be a biocontrol candidate to control fungal diseases in crops.

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Isolation and Characterization of Antifungal Substances from Burkholderia sp. Culture Broth

Cited by 52 publications

References 28 publications

Diversity of Cultivated Endophytic Bacteria from Sugarcane: Genetic and Biochemical Characterization of Burkholderia cepacia Complex Isolates

Diversity of Cultivated Endophytic Bacteria from Sugarcane: Genetic and Biochemical Characterization of Burkholderia cepacia Complex Isolates

Biocontrol Potential of Streptomyces griseus H7602 Against Root Rot Disease (Phytophthora capsici) in Pepper

Isolation and Biocontrol Potential of Bacillus amyloliquefaciens Y1 against Fungal Plant Pathogens

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