Isolation and characterization of aniline-degradingRhodococcussp. strain AN5

Abstract

A pure culture using aniline as a sole source of carbon and energy was isolated by selective enrichment culturing on a minimum salt medium (MSM) from the Nanjing Chemical Plant, Nanjing, Jiangsu Province, China. An analysis of the 16S rRNA gene sequence and morphological and physiological characteristics showed that this strain was a member of the genus Rhodococcus, and it was designated as strain AN5. Its optimal conditions for aniline biodegradation were 30 degrees C (pH 7.0). Due to the water insolubility of aniline, growth of strain AN5 at various aniline concentrations was studied via spectrophotometry and microcalorimetry in different culture mediums. Strain AN5 not only utilized aniline as its sole carbon and energy resources, but also degraded phenol, benzoic acid and naphthalin. Glucose, peptone and ammonium sulfate, when utilized as its secondary carbon and nitrogen sources, had no active effect on the aniline biodegradation. Such findings would be valuable in the application of strain AN5 to depurate industrial waste water.

Biodegradation of aniline by alkaliphilic strain Bacillus badius D1

“…The ability of Bacillus badius D1 strain to degrade aniline even at a higher % of salinity suggest that the strain is halotolerent [37] and can be used to treat the effluent containing high amount of salinity. All additional carbon sources were effective in acceleration of percentage degradation [38,35]. Similar results also observed in case of inorganic nitrogen sources.…”

“…At pH 9.0 the degradation rate was maximum while the rate was marginally decreased with increase in pH indicating that the alkaliphile, Bacillus badius D1 strain degrade aniline in a wide range of pH, while most of the neutrophilic bacteria degrade organic compound at neutral pH. Similarly, the effect of temperature on the degradation of aniline revealed that the bacterial strain could degrade aniline significantly in a wide range of temperature 0 C) indicating no thermal inactivation of bacterial degradation activity at 40 0 C. Further increase in temperature resulted in a decreased degradation rate [35]. The optimum degradation temperature [36] observed at 30 0 C. The degradation potential of this bacteria has been also studied in the presence of various salinity with additional carbon and nitrogen sources.…”

Biodegradation of Endocrine-Disrupting Chemical Aniline by Microorganisms

“…[8][9][10][11][12][13][14] Studies researching into the degradation of aniline by bacteria as the sole source of carbon have been done by many researchers. 4,8) However, few studies have focused on the processes and degradation ability of bacteria to utilize aniline as the sole source of nitrogen. In the previous paper, we reported the complete degradation of phthalic acid and dimethyl phthalate ester by Flavobacterium.…”

“…2,3) Aniline is also one of the most important chemical products in industry as synthetic intermediate. 4) Aniline is widely used in polyurethane, rubber, agricultural chemicals, and, to a limited extent, in food and medical products. Because of the global utilization of aniline and its derivatives in large quantities, they have been detected in every environment in which they have been soughed.…”

Cloning and functional analysis of the genes coding for 4-aminobenzenesulfonate 3,4-dioxygenase from Hydrogenophaga sp. PBC

“…SadB formed a monophyletic group with proteins involved in the oxidative deamination of aniline such as TdnQ, TadQ and AtdA1 (Fukumori & Saint, 1997;Liang et al, 2005;Murakami et al, 2003;Urata et al, 2004;Zhuang et al, 2007) and some uncharacterized putative glutamine-synthetaselike proteins from betaproteobacteria (Fig. 4a).…”