Isolation and characterization of anAchromobacter xylosoxidansstrain B3 and other bacteria capable to degrade the synthetic chelating agent iminodisuccinate

Abstract

Three bacterial strains were isolated, which used the synthetic chelating agent iminodisuccinate (IDS) as sole carbon source for growth in mineral salts media (MSM). Taxonomic analysis and 16S rDNA sequence analysis identified one of these isolates (B3), which was isolated from sewage sludge, as a strain of Achromobacter xylosoxidans subsp. xylosoxidans. It exhibited a doubling time of approximately 3 h in liquid MSM supplemented with IDS and grew even in the presence of 1.0% (w/v) IDS. Since photometric and high performance liquid chromatography analysis showed that IDS, which came onto the market only recently as an alternative for ethylenediaminetetraacetate, was completely degraded by axenic cultures of bacteria; it will probably be readily degraded in the environment.

Ion Exchange Technologies

“…Iminodisuccinic acid (IDS) also known as Baypure CX 100 is a medium-strong chelator consisting of: iminodisuccinic acid sodium salt > 32%, aspartic acid sodium salt < 7%, fumaric acid sodium salt < 3.5 %, hydroxysuccinic acid < 0.9 %, maleic acid sodium salt < 0.9 % (IDS Na-salz, 1998; Vasilev et al 1996;Vasilev et al 1998;Reinecke et al 2000, Kołodyńska et al 2009Kołodyńska, 2009a). Iminodisuccinic acid sodium salt can form quintuplebonded complexes with metal ions.…”

Tetrasodium iminodisuccinate as a biodegradable complexing agent for remediating metal‐contaminated soil

“…As a new type of amino carboxyl chelating agent, tetrasodium iminodisuccinate (IDS) has strong chelating properties and high biodegradability. [30][31][32] In recent years, IDS has been widely used and has gradually replaced EDTA in some industries. [33] The dissociation constants of IDS (pK) are pK 1 = 2.8, pK 2 = 3.6, pK 3 = 4.7, and pK 4 = 10.2.…”