Isolation and Characterization of an Avian Adenovirus-Associated Virus

Abstract

An 18to 20-nm virus particle was isolated from the Olson strain of quail bronchitis, an avian adenovirus. On density gradient separation the small virions were primarily found at densities of 1.39 and 1.42 g/cm3. The majority of the infectious particles were at the heavier density. The virus had a hexagonal outline and contained single-stranded deoxyribonucleic acid. It was resistant to heating at 56 C for more than an hour and was not inactivated by treatment with chloroform or low pH. Purified virus did not agglutinate erythrocytes of various avian and mammalian species. Replication of the small particles occurred either in chicken embryos or in cultures of embryo kidney cells coinfected with an adenovirus helper. Antigenically the virus was distinct from the adeno-associated viruses types 1, 2, 3, and 4. The virus is the avian equivalent of the adeno-associated viruses of primates and lower animals. The adeno-associated viruses are defective and have been found in association with the

Clinical Aspects of Immunosuppression in Poultry

“…Chicks with aplastic anemia are immunosuppressed and susceptible to adenovirus infections and bacterial disease (184,224). The adeno-associated virus reported in the early 1970's by Yates et al (275) was likely CAV.…”

Detection and serological identification of adeno-associated virus in avian adenovirus stocks

“…Apparently the small virions were in too low a concentration to detect as a distinct light-scattering band at densities of 1.39 and 1.42 g/cm3, where they appear after density gradient cen- a Using 4 to 8 U of serum to AAAV isolated from quail bronchitis virus (13). AC, Anticomplementary.…”

“…Viruses were concentrated in CsCl gradients as described by Laver et al (7). Co-infection of embryos with CELO was used for replication of AAAV (13). AAAV in adenovirus stocks was titrated by injecting serial dilutions of the stocks into 10-day-old chicken embryos co-infected with 10' plaque-forming units of CELO.…”

“…An avian adeno-associated virus (AAAV) was isolated from the Olson strain of quail bronchitis virus (13). The virus was 20 nm in diameter, hexagonal in outline, and contained singlestranded deoxyribonucleic acid.…”

Bedeutung des aviären Adeno-assoziierten Virus (AAAV) bei serologischen Untersuchungen über Adenovirus-Infektionen des Huhnes

“…Fur die meisten Fragestellungen in der Routinediagnostik durfte es jedoch vorzuziehen sein, das Vorhandensein von AAAV-Antikorpern als Indiz fur das Vorkommen einer Adenovirus-Infektion zu werten (14) und damit die Empfindlihkeit des ID-Tests indirekt zu steigern. Selbst wenn es moglich ware, mit Hilfe des ID-Tests zuverlassig zwischen verschiedenen Serotypen des Adenovirus zu unterscheiden, wurde das fur die Beurteilung der Bedenklihkeit einer Adenovirus-Infektion n i h t nutzen.…”